中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (4): 757-760.doi: 10.3969/j.issn.1673-8225.2011.04.046

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    

酸性成纤维细胞因子复合部分脱蛋白骨修复早期股骨头缺血坏死的组织学改变

朱肖奇1,2，郭  浩3，葛宝丰4   

  1. 1解放军兰州军区总院骨科博士后工作站，甘肃省兰州市  730050
    2南方医科大学附属深圳宝安医院骨外科，广东省深圳市  518101
    3南华大学，湖南省衡阳市  421001
    4解放军兰州军区总院，甘肃省兰州市  730050
  • 收稿日期:2010-08-19 修回日期:2010-11-20 出版日期:2011-01-22 发布日期:2011-01-22
  • 作者简介:朱肖奇☆，男，1964年生，博士，2004年四川大学毕业，湖南省湘潭市人，汉族，副主任医师，主要从事骨科临床和组织工程研究。
  • 基金资助:

    课题受2007年深圳市科技计划资助项目(医疗卫生类) 资助，编号：200702043。

Acidic fibroblast growth factor combined with partially deproteinized bone in repair of early-stage avascular necrosis of the femoral head in rabbits

Zhu Xiao-qi1, 2, Guo Hao3, Ge Bao-feng4   

  1. 1Post-doctoral Workstation, General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou  730050, Gansu Province, China
    2Department of Orthopedics, Shenzhen Baoan Hospital, Southern Medical University, Shenzhen  518101, Guangdong Province, China
    3Nanhua University, Hengyang  421001, Hunan Province, China
    4General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou  730050, Gansu Province, China
  • Received:2010-08-19 Revised:2010-11-20 Online:2011-01-22 Published:2011-01-22
  • About author:Zhu Xiao-qi☆, Doctor, Associate chief physician, Post-doctoral Workstation, General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu Province, China; Department of Orthopedics, Shenzhen Baoan Hospital, Southern Medical University, Shenzhen 518101, Guangdong Province, China hobartzhu@hotmail. com
  • Supported by:

    the Science and Technology Planning Project of Shenzhen, No. 200702043*

PDF

412

被引次数

3

摘要：

背景：以往研究显示，酸性成纤维细胞因子(acidic fibroblast growth factor，aFGF)复合部分脱蛋白骨(partially deproteinised bone，PDPB)对实验动物早期股骨头缺血坏死血管再生具有良好的促进作用，但其组织学变化尚不明确。
目的：从组织学变化角度观察aFGF复合PDPB修复兔早期股骨头缺血坏死的效果。
方法：用新西兰大白兔制备早期股骨头缺血坏死动物模型，建模后随机分为空白组、PDPB组和aFGF/PDPB组。PDPB组植入PDPB，aFGF/PDPB组植入aFGF/PDPB。所有动物分别于第2，4，8周取材进行苏木精伊红染色观察成骨情况。
结果与结论：空白组第8周缺损区被纤维结缔组织填充，在交界处有少量骨样组织形成。PDPB组第4周有少量新骨生成，髓腔形成，第8周较多植入物吸收，髓腔形成，有很多成骨细胞及髓细胞分布其中。aFGF/PDPB组各时间点成骨都普遍优于PDPB组，4周组移植物腔填充以骨样组织，可见较多的成骨前体细胞和骨母细胞，可见较多的微血管，骨样组织开始重建。第8周植入物已被新骨取代，髓腔已形成具有很多骨髓细胞分布其中，骨小梁交界处有大量成骨细胞，同时有少量破骨细胞存在可能参与骨塑形，骨陷窝可见成熟骨细胞。组织学检测结果提示，在修复股骨头缺血坏死的效果上，aFGF复合PDPB优于单独应用PDPB。

关键词: 酸性成纤维细胞因子, 部分脱蛋白骨, 股骨头缺血性坏死, 兔, 组织学检查

Abstract:

BACKGROUND: Previous studies have demonstrated that acidic fibroblast growth factor (aFGF) combined with partially deproteinized bone (PDPB) (aFGF/PDPB) well promotes vascularization in animals with early-stage avascular necrosis of the femoral head (ANFH), but the histological results remain unknown.
OBJECTIVE: To observe the histological repairing effects of aFGF/PDPB on early-stage ANFH in rabbits.
METHODS: New Zealand rabbits were established models of bilateral ANFH and were randomly divided into a blank group, a simple PDPB group, and an aFGF/PDPB group. PDPB and aFGF/PDPB bone were implanted into the PDPB and aFGF/PDPB group accordingly. The blank group did not receive any implantation. At 2, 4, and 8 weeks after surgery, all animals were sacrificed for histological examination to observe the osteogenesis by hematoxylin-eosin staining.
RESULTS AND CONCLUSION: Defects were filled with granulation tissues and fibrous connective tissues, only a little osteoid tissue formed at the borderline in the blank group at the end of the 8th week. In the PDPB group, a little new bone and cavitas medullaris formed. At 8 weeks, lots of graft was absorbed and cavitas medullaris formed with more osteoplasts and myeloid cells in it. The osteogenesis in the aFGF/PDPB group was better than that of PDPB group in each time point. At 4 weeks, the transplanted cavity was filled with osteoid tissues, a lot of osteogenic precursor cells and osteoblasts could be seen. Plenty of micrangium was observed, and osteoid tissues began to rebuild. At 8 weeks, the graft was replaced by bone tissues, and cavitas medullaris were formed with lots of bone marrow cells in it. At the borderline of the bone trabecula, there were lots of osteoplast and little osteoclasts, which may play a role in bone remodeling. There were mature bone cells in bone lacuna. Results indicate that aFGF/PDPB has better repair effect on rabbit model of ANFH than that of simple PDPB.

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