中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (3): 391-394.doi: 10.3969/j.issn.1673-8225.2011.03.003

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

骨髓间充质干细胞在纳米晶胶原基骨内生长以及成骨潜能

穆晓红1，赵子义2，徐  林2，王硕仁3，朱陵群3，陈  江2，李小平2   

  1. 1北京中医药大学，北京市  100029；北京中医药大学东直门医院，2骨科， 3中心实验室，北京市   100700
  • 收稿日期:2010-07-19 修回日期:2010-09-14 出版日期:2011-01-15 发布日期:2011-01-15
  • 通讯作者: 赵子义，博士，副主任医师，北京中医药大学东直门医院骨科，北京市 100700 zhaoziyi@hotmail.com
  • 作者简介:穆晓红☆，女，1972年生，河南省郑州市人，2009年北京中医药大学毕业，博士，副主任医师，主要从事干细胞生物学及神经系统损伤方面的研究。 mxh__2004@163.com
  • 基金资助:

    国家自然科学基金资助（30600847）。

Growth and osteogenesis potential of bone marrow-derived mesenchymal stem cells in nano-hydroxyapatite/collagen

Mu Xiao-hong1, Zhao Zi-yi2, Xu Lin2, Wang Shuo-ren3, Zhu Ling-qun3, Chen Jiang2, Li Xiao-ping2   

  1. 1Beijing University of Chinese Medicine, Beijing   100029, China; 2Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing   100700, China; 3Central Laboratory, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing   100700, China
  • Received:2010-07-19 Revised:2010-09-14 Online:2011-01-15 Published:2011-01-15
  • Contact: Zhao Zi-yi, Doctor, Associate chief physician, Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China zhaoziyi@hotmail.com
  • About author:Mu Xiao-hong☆, Doctor, Associate chief physician, Beijing University of Chinese Medicine, Beijing 100029, China mxh__2004@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30600847

PDF

364

被引次数

8

摘要：

背景：纳米晶胶原基骨修复材料是根据仿生原理制备的纳米骨框架材料，其微结构和成分两方面都与天然骨有相似性 ，具有良好的生物相容性。
目的：研究兔骨髓间充质干细胞与纳米晶胶原基骨修复材料体外复合培养的结合程度，以及构建组织工程骨的可行性。
方法：分离兔骨髓间充质干细胞，体外培养、纯化，取第3代骨髓间充质干细胞与纳米晶胶原基骨修复材料体外复合培养，第3，7，20天后激光共聚焦和扫描电镜观察二者复合程度。
结果与结论：骨髓间充质干细胞和纳米晶胶原基骨修复材料复合良好，共聚焦显微镜和环境扫描电镜观察均可见细胞生长；纳米晶胶原基骨修复材料能够作为良好的支架，它能使骨髓间充质干细胞在其内稳定生长。提示骨髓间充质干细胞在纳米晶胶原基骨修复材料内能很好的生长，并且具有成骨潜能。

关键词: 纳米晶胶原基骨修复材料, 骨髓间充质干细胞, 支架, 细胞培养, 组织工程骨

Abstract:

BACKGROUND: Nano-hydroxyapatite/collagen (nHAC) is a kind of nano-bone material prepared according to bionic principle, its micro-structure and compositions are closely similar to natural bone, has good biocompatibility.
OBJECTIVE: To investigate the bonding of rabbit marrow-derived mesenchymal stem cells (MSCs) and nHAC cultured in vitro, and to explore the feasibility of constructing tissue-engineering bone.
METHODS: Rabbit MSCs were isolated, cultured and purified in vitro, then rabbit MSCs of third generation were seeded onto the nHAC. Their adhesion situation was analyzed by laser confocal scanning microscope and scanning electron microscope at 3, 7, 20 days.
RESULTS AND CONCLUSION: Rabbit MSCs were well combined with nHAC, cell growth could be seen under confocal scanning microscope and scanning electron microscope; nHAC can be served as a good scaffold, it can make MSCs stably grow. Rabbit MSCs grow adherently in nHAC, and show osteogenesis potential.

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