中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (1): 37-40.doi: 10.3969/j.issn.1673-8225.2011. 01.008

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

血管内皮细胞生长因子/骨形态发生蛋白2联合修饰骨髓间充质干细胞修复股骨头坏死

崔大平1，赵德伟1，2   

  1. 1大连理工大学电子与信息工程学院，辽宁省大连市 116023
    2大连大学附属中山医院骨科，辽宁省大连市  116001
  • 收稿日期:2010-09-08 修回日期:2010-10-30 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 赵德伟，教授，大连大学附属中山医院骨科，辽宁省大连市 116001 deweizhao.dush@gmail.com
  • 作者简介:崔大平☆，男，1978年生，辽宁省大连市人，汉族，大连理工大学在读博士，主治医师，主要从事关节外科研究。 cuidaping@126.com
  • 基金资助:

    国家自然科学基金项目(30471752)，骨髓基质干细胞体外转导修饰关节镜监视下自体回植治疗股骨头缺血性坏死的研究。

Vascular endothelial cell growth factor combined with bone morphogenetic protein-2-modified bone marrow mesenchymal stem cells in repair of femoral head necrosis

Cui Da-ping1, Zhao De-wei1,2   

  1. 1Dalian University of Technology, Dalian 116023, Liaoning Province, China;
    2Department of Orthopaedics, Zhongshan Hospital of Dalian University, Dalian  116001, Liaoning Province, China
  • Received:2010-09-08 Revised:2010-10-30 Online:2011-01-01 Published:2011-01-01
  • Contact: Zhao De-wei, Professor, Dalian University of Technology, Dalian 116023, Liaoning Province, China; Department of Orthopaedics, Zhongshan Hospital of Dalian University, Dalian 116001, Liaoning Province, China deweizhao.dush@gmail.com
  • About author:Cui Da-ping☆, Studying for doctorate, Attending physician, Dalian University of Technology, Dalian 116023, Liaoning Province, China cuidaping@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30471752

PDF

616

被引次数

42

摘要：

背景：将血管内皮细胞生长因子及骨形态发生蛋白二者联合修复坏死骨，有可能在保持种子细胞成骨表型的同时，又能持续高效地分泌血管内皮细胞生长因子及骨形态发生蛋白2，从而有效促进血管再生，促进组织工程化骨组织的形成和再血管化。
目的：观察血管内皮细胞生长因子165/骨形态发生蛋白2体外修饰骨髓间充质干细胞移植治疗兔股骨头缺血性坏死效果。
方法：建立兔右侧股骨头缺血性坏死模型，并以抽签法随机分为3 组：单纯髓芯减压组、髓芯减压+骨髓间充质干细胞组、髓芯减压+血管内皮细胞生长因子165/骨形态发生蛋白2转染骨髓间充质干细胞组。关节镜监视下将坏死骨清除，组织学检查成骨及血管生成情况。
结果与结论：通过关节镜观察显示各组坏死骨清除干净，有新鲜血流出。组织形态学检测发现，髓芯减压+血管内皮细胞生长因子165/骨形态发生蛋白2转染骨髓间充质干细胞组移植后不同时间点血管数量及修复区新骨面积比显著高于单纯髓芯减压组、髓芯减压+骨髓间充质干细胞组(P < 0.05)。提示血管内皮细胞生长因子165/骨形态发生蛋白2基因转染加强了骨髓间充质干细胞成骨作用，提高了新生骨的数量与质量，加快了股骨头缺血性坏死的修复。

关键词: 股骨头坏死, 干细胞, 转染, 血管内皮细胞生长因子, 骨形态发生蛋白2

Abstract:

BACKGROUND: Vascular endothelial cell growth factor (VEGF) combined with bone morphogenetic protein (BMP) was used to repair necrotic bone, which can maintain the osteogenic phenotype of seed cells, and effectively secrete VEGF and BMP-2, and effectively promote blood vessel regeneration and contribute to formation and revascularization of tissue engineered bone tissues.
OBJECTIVE: To observe the therapeutic effect on the treatment of avascular necrosis of the femoral head by using bone marrow mesenchymal stem cells (BMSCs) modified by VEGF-165 and BMP-2 in vitro.
METHODS: The model of avascular necrosis of rabbit femoral head on right leg. There were single core decompression group, core decompression and BMSCs group, decompression with VEGF-165/BMP-2/BMSCs group. Necrotic bone was cleared out on arthroscope. Osteogenesis and angiogenesis were inspected by histology.
RESULTS AND CONCLUSION: Arthroscope observation demonstrated that necrotic bone was cleared out in each group, and fresh blood was flowed out. Histomorphology determination showed that blood vessel number and new bone area in the repair region were significantly greater at various time points following transplantation in the decompression with VEGF-165/BMP-2/BMSCs group compared with single core decompression group and core decompression and BMSCs group (P < 0.05). These suggested that VEGF-165/BMP-2 gene transfection strengthened osteogenic effects of BMSCs, elevated number and quality of new bones and accelerated the repair of avascular necrosis of the femoral head.

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