中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (53): 9897-9901.doi: 10.3969/j.issn.1673-8225.2010.53.004

• 心肺移植 heart-lung transplantation • 上一篇    下一篇

肺移植前后可溶性细胞间黏附分子1的变化

张  笛1，虞竞峰2，肖华龙2   

  1. 南京医科大学附属无锡市人民医院，1内科，2医学检验科，江苏省无锡市  214023
  • 出版日期:2010-12-31 发布日期:2010-12-31
  • 通讯作者: 肖华龙，主任技师，副教授，硕士生导师，南京医科大学附属无锡市人民医院医学检验科，江苏省无锡市 214023 xhl0510@163.com
  • 作者简介:张笛，男，1959年生，江苏省无锡市人，汉族，1982年南通医学院临床医疗系毕业，主任医师，主要从事移植排斥反应及抗排斥反应治疗方面的研究， gxh913@163.com
  • 基金资助:

    该课题为2005年卫生部核医学重点实验室开放课题(项目编号：WK005-021)主要研究可溶性细胞间粘附分子免疫分析方法的建立及其在临床上的应用。

Fluctuation of soluble intercellular adhesion molecule-1 prior to and after lung transplantation

Zhang Di1, Yu Jing-feng2, Xiao Hua-long2   

  1. 1 Department of Internal Medicine, 2 Department of Laboratory Medicine, Wuxi People’s Hospital, Nanjing Medical University, Wuxi  214023, Jiangsu Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Xiao Hua-long, Chief technician, Associate professor, Master’s supervisor, Department of Laboratory Medicine, Wuxi People’s Hospital, Nanjing Medical University, Wuxi 214023, Jiangsu Province, China
  • About author:Zhang Di, Chief physician, Department of Internal Medicine, Wuxi People’s Hospital, Nanjing Medical University, Wuxi 214023, Jiangsu Province, China gxh913@163.com
  • Supported by:

    the Open Subjects for Key Laboratory of Nuclear Medicine, Ministry of Public Health, No. WK005-021*

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摘要：

背景：应用生物素-链亲和素系统建立的可溶性细胞间黏附分子1测定法，是一种敏感度高、可测范围广的检测方法。
目的：建立可溶性细胞间黏附分子1生物素-链亲和素系统时间分辨荧光免疫分析方法并应用于临床，探讨肺移植前后血清可溶性细胞间黏附分子1的变化及临床意义。
方法：使用2株匹配的单克隆抗体分别作为捕获抗体和检测抗体，利用制备的铕标记链亲和素(SA-Eu 3+ )作为示踪物并与生物素化的检测抗体特异结合，建立双位点多层夹心法，通过对30例健康人血清可溶性细胞间黏附分子1检测和26例肺移植受者手术前后血清可溶性细胞间黏附分子1的变化测定，对可溶性细胞间黏附分子1生物素-链亲和素系统方法学和在肺移植术前后的临床应用价值进行评价。
结果与结论：30例健康对照测定结果为(348.63±69.12) µg/L。移植前可溶性细胞间黏附分子1与对照组无显著性差别；移植后，各组与对照组之间比较差异有显著性意义(P < 0.05)，急性排斥反应时血清可溶性细胞间黏附分子1升高，并发感染时降低，但各组之间比较无明显差别。表明可溶性细胞间黏附分子1生物素-链亲和素系统是一种新型的高灵敏度、宽范围的非放射性标记免疫分析法，对肺移植受者移植前后监测血清可溶性细胞间黏附分子1可作为辅助诊断急性排斥反应的免疫学指标。

关键词: 可溶性细胞间黏附分子1, 时间分辨免疫荧光分析法, 生物素, 链亲和素, 双位点多层夹心法, 肺移植

Abstract:

BACKGROUND: The soluble intercellular adhesion molecule-1 (sICAM-1) assay is based on biotin-streptavidin (BSA) system and has been widely used due to high sensitivity. 
OBJECTIVE: To establish sICAM-1 BAS time resolution fluoroimmunoassay method (sICAM-1 BAS-TRFIA) and to explore the sICAM-1 fluctuation and clinical significance prior to and after lung transplantation.
METHODS: Two matching monoclonal antibodies served as capture immune body and the examination immune body, respectively. The europium-labeled streptavidin (SA-Eu 3+ ) served as tracer and combined with biotinylated immune body to establish the sICAM-1 BSA-TRFIA method. This method was used to detect sICAM-1 levels in 30 healthy adults and 26 recipients prior to and after lung transplantation. The clinical significance of sICAM-1 BSA-TRFIA method was evaluated.
RESULTS AND CONCLUSION: The determine results of 30 healthy adults were (348.63±69.12) µg/L. There was no significant difference in sICAM-1 between control and prior to transplantation group. After transplantation, there was obviously difference  (P < 0.05). The sICAM-1 increased when acute rejection occurs but decreased after concurrent infection, but the differences had no significant. The findings demonstrated that, sICAM-1 BSA-TRFIA method is a novel non-radioactive labeled immunoassay with high sensitivity and wide scope. Monitoring of serum sICAM-1 is helpful to auxiliary diagnosis acute rejection following lung transplantation.

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