中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (31): 5753-5757.doi: 10.3969/j.issn.1673-8225.2010.31.014

• 细胞与组织移植 cell and tissue transplantation • 上一篇    下一篇

高氧液对大鼠胰腺移植的保护作用　

梁忠平1，劳学军2，丁泓文2，苏泽轩2,3　   

  1. 1清远市人民医院，暨南大学第五附属医院胃肠外科，广东省清远市 511500；2暨南大学第一附属医院移植中心，广东省广州市 510632；3南方医科大学研究生学院，广东省广州市  510525
  • 出版日期:2010-07-30 发布日期:2010-07-30
  • 通讯作者: 苏泽轩，教授，博士生导师，暨南大学第一附属医院器官移植中心，广东省广州市 510630 suz2008@126.com
  • 作者简介:梁忠平☆，男，1973年生，江西省会昌县人，汉族，2009年毕业于南方医科大学，博士，主治医师，主要从事器官移植方向的研究 liangzp2006@126.com
  • 基金资助:

    广东省自然科学基金资助项目(04010486)；广东省卫生厅基金资助项目(A2004337)。

Protective effect of hyperoxic fluid on pancreas transplantation in rats

Liang Zhong-ping1, Lao Xue-jun2, Ding Hong-wen2, Su Ze-xuan 2,3   

  1. 1 Department of Gastrointestinal Surgery, Qingyuan People’s Hospital, Fifth Affiliated Hospital of Jinan University, Qingyuan  511500, Guangdong Province, China; 2 Transplantation Center, First Affiliated Hospital of Jinan University, Guangzhou  510632, Guangdong Province, China; 3 Graduate College of Southern Medical University, Guangzhou  510525, Guangdong Province, China
  • Online:2010-07-30 Published:2010-07-30
  • Contact: Su Ze-xuan, Professor, Doctoral supervisor, Transplantation Center, First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China; Graduate College of Southern Medical University, Guangzhou 510525, Guangdong Province, China suz2008@126.com
  • About author:Liang Zhong-ping☆, Doctor, Attending physician, Department of Gastrointestinal Surgery, Qingyuan People’s Hospital, Fifth Affiliated Hospital of Jinan University, Qingyuan 511500, Guangdong Province, China liangzp2006@126.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 04010486*; Foundation of Health Department of Guangdong Province, No. A2004337*

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摘要：

背景：目前可供移植的器官日益短缺，但因灌注冷保存所至的原发性移植物无功能仍然有一定的发生率，减少灌注保存所至的器官损伤有较大的临床意义。
目的：探讨高氧液对大鼠胰腺移植的保护作用。
方法：40只SPF级Wistar 大鼠，随机分成2组(n=20)，高氧液组灌注高氧液，对照组灌注普通林格液，比较两组灌注后胰尾组织电镜结果、移植后第1天、3天血血糖浓度，以及两组移植后胰腺组织病理急性排斥评分。
结果与结论：移植前两组差异无显著性意义，灌注后高氧液组胰腺组织超微结构基本正常，细胞核形态完整，大量线粒体存在；对照组胰腺组织细胞大部分排列仍有序整齐，胞浆内线粒体水肿明显，部分线粒体空泡变性。移植后第1，3天血血糖高氧液组低于对照组(P < 0.05)；高氧液组评分低于对照组(P < 0.05)。可见高氧液可减轻缺血再灌注损伤，对大鼠胰腺移植有一定的保护作用。

关键词: 高氧液, 器官移植, 胰腺移植, 急性排斥反应, 保护

Abstract:

BACKGROUND: Organ for transplantation is insufficient, and primary transplantation of nonfunction caused by perfusion cryopreservation occasionally occurs. It has clinically significant to reduce organ damage caused by perfusion preservation.
OBJECTIVE: To study the protective effect of hyperoxic perfusion fluid on pancrease transplantation in rats.
METHODS: Totally 40 Wistar rats were randomly divided two groups and poured with natrium lacticum ringer’s solution or hyperoxic natrium lacticum ringer’s solution. After perfusion, the tail pancreases of rats were checked by electron microscopy. The glucose concentrations and acute rejection score of pancreas tissues were compared between two groups at 1 and 3 days after pancreas transplantation.
RESULTS AND CONCLUSION: There was no statistics difference between two groups prior to operation. The ultrastructure of pancreatic tissue was basically normal, with completely nuclei and plenty of mitochondria in the hyperoxia liquid group after perfusion. The pancreatic tissues still orderly arranged in the control group, with obviously swelling in mitochondria, and parts of mitochondria were vacuolar degenerated. The glucose concentrations of the hyperoxia liquid group were lower than that of the control group at 1 and 3 days after transplantation (P < 0.05). The acute rejection scores of the hyperoxia liquid group were lower than that of the control group (P < 0.05). Thus, hyperoxia liquid can mitigate the ischemia reperfusion injury and protect pancreas transplantation in rats.

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