中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (31): 5729-5732.doi: 10.3969/j.issn.1673-8225.2010.31.008

• 肝移植 liver transplantation • 上一篇    下一篇

肝移植后受者淋巴细胞亚群表达与免疫平衡

张永明，胡维昱，李  勇，郝建帅，吴力群   

  1. 青岛大学医学院附属医院肝胆外科，山东省青岛市   266003
  • 出版日期:2010-07-30 发布日期:2010-07-30
  • 通讯作者: 吴力群，博士，博士生导师，主任医师，青岛大学医学院附属医院肝胆外科，山东省青岛市 266003 wulq5810@126.com
  • 作者简介:张永明★，男，1982年生，山东省烟台市人，汉族，2010年青岛大学医学院毕业，硕士，主要从事肝胆外科方面的研究。 zym19820809@126.com
  • 基金资助:

    2006年青岛市科技发展重大项目攻关项目(06-2-2-6-nsh-1)。

Expression of lymphocyte subsets and immune balance in recipients following liver transplantation

Zhang Yong-ming, Hu Wei-yu, Li Yong, Hao Jian-shuai, Wu Li-qun   

  1. Department of Hepatobiliary Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao  266003, Shandong Province, China
  • Online:2010-07-30 Published:2010-07-30
  • Contact: Wu Li-qun, Doctor, Doctoral supervisor, Chief physician, Department of Hepatobiliary Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China wulq5810@126.com
  • About author:Zhang Yong-ming★, Master, Department of Hepatobiliary Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China zym19820809@126.com
  • Supported by:

    the Key Tackle Item for Science and Technology Development of Qingdao, No. 06-2-2-6-nsh-1*

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摘要：

背景：有文献报道，肝移植后受者外周血淋巴细胞的变化较肝脏酶学的改变更为敏感，其特异性有待进一步研究。
目的：分析淋巴细胞亚群中CD3- /HLA-DR+ ，CD3+ /CD25+和CD3+ /HLA-DR-与肝移植受者机体免疫状况和并发症的关系。
方法：应用全自动生化分析仪和流式细胞分析仪检测56例肝移植受者移植后肝功能和淋巴细胞亚群，依照肝功能情况划分为肝功能正常组52例和肝功能异常组27例，肝功能异常组中分为急性排斥组7例、药物反应组11例和原因不明组9例。分析各组肝移植受者CD3- /HLA-DR+ ，CD3+ /CD25+和CD3+ /HLA-DR表达水平与其并发症的关系。
结果与结论：肝功能正常组CD3- /HLA-DR+和CD3+ /CD25+的表达水平低于肝功能异常组(P=0.011，0.002)，CD3+ /HLA-DR-的表达高于肝功能异常组(P=0.012)。CD3- /HLA-DR+和CD3+ /CD25+在急性排斥组的表达水平明显高于药物反应组(P=0.039，0.048)，急性排斥组CD3+ /HLA-DR-的表达水平低于药物反应组(P=0.007)。提示CD3- /HLA-DR+ ，CD3+ /CD25+和CD3+ /HLA-DR-的表达水平与肝移植后受者机体免疫状况及并发症密切相关，可作为判断肝移植后受者并发症的辅助指标以及进行免疫干预的参考依据。

关键词: 肝移植, CD3- /HLA-DR+ , CD3+ /CD25+ , CD3+ /HLA-DR- , , 排斥, 药物反应

Abstract:

BACKGROUND: Documents reported that the peripheral blood lymphocyte changes are more sensible than hepatic enzymes changes following liver transplantation, but its specificity needs further explored.
OBJECTIVE: To study the relationship among the levels of CD3-/HLA-DR+, CD3+/CD25+ and CD3+/HLA-DR-, the immune status of the recipients, and complications in recipients after liver transplantation.
METHODS: The liver functions and lymphocyte subsets in 56 recipients were detected by the automatic biochemical analyzer and flowcytometry. The recipients were divided into 2 groups (normal and abnormal groups) according to liver function. And the recipients in the abnormal group were divided into the acute rejection group (n=7), drug reaction group (n=11) and unknown reasons group (n=9). The levels of CD3-/HLA-DR+, CD3+/CD25+ and CD3+/HLA-DR- in patients after liver transplantation of each group were measured, and  the relationship of their levels and complications were analyzed.
RESULTS AND CONCLUSION: The expression of CD3-/HLA-DR+ and CD3+/CD25+ in the normal group was lower than that in the abnormal group (P =0.011, 0.002), while the expression of CD3+/HLA-DR- was higher in the normal group (P=0.012). And the expression of CD3-/HLA-DR+ and CD3+/CD25+ in the acute rejection group was greater than those in the drug reaction group (P=0.039, 0.048), but the expression of CD3+/HLA-DR- was higher in acute rejection group (P=0.007). There were a significantly correlations between the expression of CD3-/HLA-DR+, CD3+/CD25+ and CD3+/HLA-DR- and the immune status and complications of recipients, they may be a factor to assess the complications and adjust the immune status of recipients after liver transplantation.

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