中国组织工程研究

中国组织工程研究 ›› 2026, Vol. 30 ›› Issue (30): 7902-7909.doi: 10.12307/2026.962

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

羧甲基纤维素基水凝胶的制备及性能分析

王  鑫1，2，张美婷1，2，孟子鹤1，王欣乐1，2，李  敏1，王思妍1，扈瑞平1，2，盛守新1，2，薛慧婷1，2   

  1. 1内蒙古医科大学基础医学院，内蒙古自治区呼和浩特市   010030；2内蒙古医科大学基础医学院分子生物学研究中心，内蒙古自治区呼和浩特市   010030
  • 收稿日期:2026-06-02 修回日期:2026-06-18 出版日期:2026-10-28 发布日期:2026-09-12
  • 通讯作者: 薛慧婷，博士，教授，内蒙古医科大学基础医学院，内蒙古自治区呼和浩特市 010030；内蒙古医科大学基础医学院分子生物学研究中心，内蒙古自治区呼和浩特市 010030 通讯作者：盛守新，博士，内蒙古医科大学基础医学院，内蒙古自治区呼和浩特市 010030；内蒙古医科大学基础医学院分子生物学研究中心，内蒙古自治区呼和浩特市 010030
  • 作者简介:王鑫，男，2001年生，内蒙古自治区赤峰市人，汉族，内蒙古医科大学基础医学院在读硕士，主要从事糖复合物的结构与功能研究。
  • 基金资助:
    国家自然科学基金项目(32460231)，项目负责人：薛慧婷；内蒙古自治区青年拔尖人才项目，项目负责人：薛慧婷；内蒙古医科大学致远人才项目(ZY20242103)，项目负责人：薛慧婷；内蒙古医科大学面上项目(2025MS08075)，项目负责人：
    薛慧婷；内蒙古自治区高校青年教师科研创新能力支持项目，项目负责人：薛慧婷

Preparation and performance analysis of composite hydrogels based on carboxymethyl cellulose

Wang Xin1, 2, Zhang Meiting1, 2, Meng Zihe1, Wang Xinle1, 2, Li Min1, Wang Siyan1, Hu Ruiping1, 2, Sheng Shouxin1, 2, Xue Huiting1, 2   

  1. 1School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; 2Molecular Biology Research Center, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • Received:2026-06-02 Revised:2026-06-18 Online:2026-10-28 Published:2026-09-12
  • Contact: Xue Huiting, PhD, Professor, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; Molecular Biology Research Center, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Co-corresponding author: Sheng Shouxin, PhD, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; Molecular Biology Research Center, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • About author:Wang Xin, MS candidate, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China; Molecular Biology Research Center, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
  • Supported by:
    National Natural Science Foundation of China, No. 32460231 (to XHT); Inner Mongolia Autonomous Region Young Elite Talent Program (to XHT); Inner Mongolia Medical University Zhiyuan Talent Program, No. ZY20242103 (to XHT); Inner Mongolia Medical University General Project, No. 2025MS08075 (to XHT); Inner Mongolia Autonomous Region Higher Education Institution Young Faculty Research and Innovation Capacity (to XHT)

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摘要：



文题释义：
羧甲基纤维素：天然纤维素经羧甲基化改性制备的阴离子多糖，分子链上富含大量羟基、羧基等亲水基团，因具有优异的亲水性、生物相容性被广泛应用于天然多糖基生物材料。
物理交联：指不使用化学交联剂的情况下，仅通过氢键、离子配位等非共价键使高分子链之间形成稳定三维结构的交联方式，具有无毒、生物相容性高等特点。

背景：水凝胶凭借高吸水性、高保湿性和良好的生物相容性，被认为是理想的伤口敷料。
目的：构建羧甲基纤维素基水凝胶，表征其生物安全性与生物学性能。
方法：①以羧甲基纤维素为凝胶骨架，聚乙二醇和聚乙烯吡咯烷酮为交联剂，采用加热法制备羧甲基纤维素基水凝胶。采用傅里叶红外光谱、扫描电镜、流变学和热重分析系统表征羧甲基纤维素基水凝胶的结构与性能，并进一步评估其溶胀行为和保湿能力。②将小鼠胚胎成纤维细胞系NIH/3T3分别与不同质量浓度(2.5，5，10 mg/mL)的羧甲基纤维素基水凝胶浸提液共培养，CCK-8法检测细胞活力，划痕实验检测细胞迁移率。将不同质量浓度(2.5，5，10 mg/mL)的羧甲基纤维素基水凝胶浸提液分别与小鼠悬浮红细胞共培养，检测溶血率。将金黄色葡萄球菌(或大肠杆菌)菌液与羧甲基纤维素基水凝胶共培养，通过涂布平板抗菌实验检测抑菌率。
结果与结论：①羧甲基纤维素基水凝胶主要通过氢键作用实现网络交联，扫描电镜下呈现致密多孔微观形貌，流变学与热重分析显示该材料具备良好的机械强度与热稳定性，溶胀性能与保湿性能分析显示该材料可在短时间内快速吸液并维持长效湿润状态，为伤口处提供湿润环境，有利于伤口愈合。②CCK-8检测显示不同质量浓度羧甲基纤维素基水凝胶无明显的细胞毒性，溶血实验显示不同质量浓度羧甲基纤维素基水凝胶无溶血现象，说明该材料具有良好的生物安全性。划痕实验显示不同质量浓度羧甲基纤维素基水凝胶浸提液可促进NIH/3T3细胞迁移。涂布平板抗菌实验显示羧甲基纤维素基水凝胶可抑制金黄色葡萄球菌与大肠杆菌的生长。③结果表明，羧甲基纤维素基水凝胶具有良好的理化性能、生物安全性及抗菌特性。

https://orcid.org/0009-0006-6801-9893 (王鑫) 


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 羧甲基纤维素, 水凝胶, 抗菌, 敷料, 性能分析, 生物相容性

Abstract: BACKGROUND: Hydrogels are considered ideal wound dressings due to their high water absorption, high moisture retention, and good biocompatibility.
OBJECTIVE: To construct a carboxymethyl cellulose-based composite hydrogel and characterize its biosafety and biological properties.
METHODS: (1) Using carboxymethyl cellulose as the gel backbone, and polyethylene glycol and polyvinylpyrrolidone as crosslinking agents, a carboxymethyl cellulose-based composite hydrogel was prepared via a heating method. The structure and properties of the carboxymethyl cellulose-based composite hydrogel were characterized using Fourier transform infrared spectroscopy, scanning electron microscopy, rheology, and thermogravimetric analysis. Its swelling behavior and moisture retention capacity were further evaluated. (2) Mouse embryonic fibroblast cell line NIH/3T3 was co-cultured with different mass concentrations (2.5, 5, 10 mg/mL) of carboxymethyl cellulose-based hydrogel extracts. Cell viability was assessed using the cell counting kit-8 assay, and cell migration rate was evaluated using the scratch assay. Mouse suspended red blood cells were co-cultured with different mass concentrations (2.5, 5, 10 mg/mL) of carboxymethyl cellulose-based hydrogel extracts to measure the hemolysis rate. Staphylococcus aureus (or Escherichia coli) bacterial suspension was co-cultured with the carboxymethyl cellulose-based hydrogel, and the antibacterial rate was determined using the spread plate method.
RESULTS AND CONCLUSION: (1) The carboxymethyl cellulose-based hydrogel achieved network crosslinking mainly through hydrogen bonding. Scanning electron microscopy revealed a dense porous microstructure. Rheology and thermogravimetric analyses showed that the material possessed good mechanical strength and thermal stability. Swelling and moisture retention analyses indicated that the material could rapidly absorb fluid within a short time and maintain a long-term moist state, providing a humid environment conducive to wound healing. (2) The cell counting kit-8 assay showed that different mass concentrations of the carboxymethyl cellulose-based composite hydrogel had no obvious cytotoxicity. The hemolysis test showed no hemolytic phenomenon at different mass concentrations, indicating good biosafety of the material. The scratch assay showed that extracts of the carboxymethyl cellulose-based hydrogel at different mass concentrations promoted NIH/3T3 cell migration. The spread plate antibacterial experiment showed that the carboxymethyl cellulose-based hydrogel inhibited the growth of Staphylococcus aureus and Escherichia coli. These findings indicate that the carboxymethyl cellulose-based hydrogel has favorable physicochemical properties, biosafety, and antibacterial characteristics.


Key words: carboxymethyl cellulose, hydrogel, antibacterial, dressing, performance analysis, biocompatibility

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