中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (14): 2186-2191.doi: 10.3969/j.issn.2095-4344.2017.14.009

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米羟基磷灰石/大管径TiO2纳米管复合涂层促成骨

雷则鸣1,张杭州2,田  昂3,尤俊华4,史晓国3,刘兴旺1,魏  博1,白希壮1   

  1. 1中国医科大学人民医院运动医学与关节外科,辽宁省沈阳市  110016;2中国医科大学附属第一医院运动医学与关节外科,辽宁省沈阳市  110001;3东北大学材料与冶金学院,辽宁省沈阳市  110819;4沈阳工业大学材料学院,辽宁省沈阳市  110870
  • 收稿日期:2016-12-20 出版日期:2017-05-18 发布日期:2017-06-10
  • 通讯作者: 白希壮,教授,中国医科大学人民医院运动医学与关节外科,辽宁省沈阳市 110016
  • 作者简介:雷则鸣,男,1990年生,辽宁省沈阳市人,汉族,中国医科大学人民医院在读博士,主要从事运动医学与关节疾病、骨科领域生物材料研究。
  • 基金资助:

    国家自然科学基金(81671811);国家青年科学基金(81501857);辽宁省省直医院改革重点临床科室诊疗能力建设项目(LNCCC-A03-2014);沈阳市人口与健康科技攻关专项(F15-139-9-23)

Large-diameter TiO2 nanotubes with nano-hydroxyapatite offer an improvement in bone-forming ability

Lei Ze-ming1, Zhang Hang-zhou2, Tian Ang3, You Jun-hua4, Shi Xiao-guo3, Liu Xing-wang1, Wei Bo1, Bai Xi-zhuang1   

  1. 1 Department of Sports Medicine/Joint Surgery, People’s Hospital of China Medical University, Shenyang 110016, Liaoning Province, China; 2 Department of Sports Medicine/Joint Surgery, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China; 3 School of Materials and Metallurgy, Northeastern University, Shenyang 110819, Liaoning Province, China; 4 School of Material Sciences, Shenyang University of Technology, Shenyang 110870, Liaoning Province, China
  • Received:2016-12-20 Online:2017-05-18 Published:2017-06-10
  • Contact: Bai Xi-zhuang, Professor, Department of Sports Medicine/Joint Surgery, People’s Hospital of China Medical University, Shenyang 110016, Liaoning Province, China
  • About author:Lei Ze-ming, Studying for doctorate, Department of Sports Medicine/Joint Surgery, People’s Hospital of China Medical University, Shenyang 110016, Liaoning Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81671811; the National Science Fund for the Youth, No. 81501857; Diagnosis and Treatment Capacity Construction for Key Clinical Departments in the Liaoning Provincial Hospital Reform, No. LNCCC-A03-2014; Special Science and Technology Research Project for Population and Health in Shenyang, No. F15-139-9-23

摘要:

文章快速阅读

 

文题释义:
TiO2纳米管:前期大量研究皆表明,通过阳极氧化法制备的TiO2纳米管具有良好生物相容性和促进细胞成骨的能力,而且有研究证实大管径纳米管更有利于细胞的成骨分化,并且可加快羟基磷灰石的沉积速率。本组实验通过电化学方法于大管径纳米管沉积羟基磷灰石,成功构建纳米羟基磷灰石/大管径TiO2纳米管复合涂层并研究前成骨细胞MC3T3-E1对纳米羟基磷灰石/大管径TiO2纳米管复合涂层的生物学反应。

背景:羟基磷灰石和大管径TiO2纳米管均被证实具有良好的生物活性,但目前缺乏在大管径TiO2纳米管表面沉积纳米羟基磷灰石促成骨方面的研究。
目的:检测纳米羟基磷灰石/大管径TiO2纳米管复合涂层的促成骨能力。
方法:采用阳极氧化法制备大管径TiO2纳米管,电化学法于纳米管表面沉积纳米羟基磷灰石。将MC3T3-E1前成骨细胞分别与纳米羟基磷灰石/大管径TiO2纳米管复合涂层、纯钛及大管径TiO2纳米管涂层共培养,培养0.5,1,2 h,观察细胞初始黏附;培养1,3,5 d后,检测细胞增殖;培养2 d后,观察细胞形态;成骨诱导培养3,7 d后,检测细胞碱性磷酸酶活力;成骨诱导培养14 d后,检测细胞外基质矿化能力。
结果与结论:①培养2 h时,TiO2纳米管组细胞数高于纳米羟基磷灰石/大管径TiO2纳米管组(P < 0.05),纳米羟基磷灰石/大管径TiO2纳米管组与纯钛组比较差异无显著性意义;②培养1,3,5 d时,TiO2纳米管组细胞增殖数量高于纳米羟基磷灰石/大管径TiO2纳米管组、纯钛组(P < 0.05),纳米羟基磷灰石/大管径TiO2纳米管组与纯钛组比较差异无显著性意义;③纯钛上的细胞呈梭形;TiO2纳米管涂层上的细胞有丝状伪足伸出;纳米羟基磷灰石/大管径TiO2纳米管复合涂层上的细胞呈多边形,伸出的伪足数量更多;④纳米羟基磷灰石/大管径TiO2纳米管组细胞内碱性磷酸酶活性、细胞外基质矿化程度明显高于TiO2纳米管组、纯钛组;⑤结果表明,纳米羟基磷灰石/大管径TiO2纳米管复合涂层不仅具有良好的生物相容性,而且具有理想的促成骨能力。

ORCID: 0000-0002-9338-8643(白希壮)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

关键词: 生物材料, 纳米材料, 羟基磷灰石, 钛钠米管, 前成骨细胞, 生物相容性, 成骨, 国家自然科学基金

Abstract:

BACKGROUND: Both hydroxyapatite (HA) and large diameter TiO2 nanotubes have excellent biocompatibility, but bone-forming ability of nano-HA (nHA) deposited large diameter TiO2 nanotubes is rarely reported.
OBJECTIVE: To evaluate the bone-forming ability of nHA/large-diameter TiO2  nanotube composite coating.
METHODS: Large-diameter TiO2  nanotubes were prepared by anodic oxidation method, and then nHA was electrochemically deposited on the surface of TiO2  nanotubes. Preosteoblasts MC3T3-E1 were co-cultured with the nHA/large diameter TiO2  nanotube composite, pure titanium and TiO2  nanotube coatings, respectively. At 0.5, 1, 2 hours after culture, the initial cell adhesion was observed. At 1, 3, 5 day after culture, cell proliferation was assessed. At 2 days after culture, cell morphology was observed. At 3 and 7 days after osteogenic induction, intracellular alkaline phosphatase activity was detected. At 14 days after osteogenic induction, mineralization of extracellular matrix was detected.
RESULTS AND CONCLUSION: (1) After 2 hours of culture, the number of adherent cells on the composite coating was significantly lower than that on the TiO2 nanotube coating (P < 0.05), but slightly higher than that on the pure titanium coating with no statistical difference. (2) After 1, 3, 5 days of culture, the cell proliferation on the composite coating was significantly lower than that on the TiO2 nanotube coating (P < 0.05), but slightly higher than that on the pure titanium with no statistical difference. (3) The cells on the pure titanium showed a spindle-shape, while those on the TiO2 nanotube coating processed filopodia. The cells on the composite coating showed polygonal shape with a larger number of filopodia. (4) The intracellular alkaline phosphatase activity of the composite coating group was significantly higher than that of the pure titanium group and TiO2  nanotube group. The trend of mineralization of extracellular matrix was ranked from high to low: the composite coating group > TiO2  nanotube group > pure titanium group. To conclude, the nHA/large diameter TiO2  nanotube composite coating not only has good biocompatibility, but also has the ideal ability to promote bone formation.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Durapatite, Nanotubes, Osteogenesis, Tissue Engineering

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