中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (14): 2280-2284.doi: 10.3969/j.issn.2095-4344.2017.14.024

• 生物材料综述 biomaterial review • 上一篇    下一篇

蚕丝丝素蛋白/壳聚糖支架在骨科再生医学中的作用与特点

袁振中,陈跃平   

  1. 广西中医药大学附属瑞康临床医学院,广西壮族自治区南宁市  530011
  • 收稿日期:2016-12-23 出版日期:2017-05-18 发布日期:2017-06-10
  • 通讯作者: 陈跃平,硕士生导师,主任医师,广西中医药大学附属瑞康医院,广西壮族自治区南宁市 530011
  • 作者简介:袁振中,男,瑶族,广西中医药大学硕士,主要从事骨与关节及运动系统损伤研究。
  • 基金资助:

    广西卫生适宜技术研究与开发项目(S201419-5);广西自然科学基金项目(2015GXNSFAA139136);广西高校青年教师基础能力提升项目(KY2016YB204);广西中医药大学校级科研项目(2015LX027);2014年广西中医药大学校级培育学科-骨外科学建设项目[J13167(7)]

Application and characteristics of silk fibroin/chitosan scaffold in orthopaedic regenerative medicine

Yuan Zhen-zhong, Chen Yue-ping   

  1. Ruikang Clinical College, Guangxi University of Chinese University, Nanning 530011, Guangxi Zhuang Autonomous Region, China
  • Received:2016-12-23 Online:2017-05-18 Published:2017-06-10
  • Contact: Chen Yue-ping, Master’s supervisor, Chief physician, Ruikang Clinical College, Guangxi University of Chinese University, Nanning 530011, Guangxi Zhuang Autonomous Region, China
  • About author:Yuan Zhen-zhong, Master, Ruikang Clinical College, Guangxi University of Chinese University, Nanning 530011, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    the Health Technology Research and Development Project of Guangxi Zhuang Autonomous Region, No. S201419-5; the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 2015GXNSFAA139136; the Basic Ability Improvement Project of Young Teachers in Guangxi Colleges and Universities, No. KY2016YB204; the Scientific Research Project of Guangxi University of Chinese Medicine, No. 2015LX027; the Training Discipline-Bone Surgery Construction Project of Guangxi University of Chinese Medicine in 2014, No. J13167(7)

摘要:

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文题释义:
蚕丝丝素蛋白/壳聚糖支架的性能
:壳聚糖作为组织工程材料其有着广泛的生物学特性,通过改变壳聚糖的分子量及脱乙酰作用程度等可以获得不同的特性,如有良好的机械性、骨诱导性、低免疫原性等特性,此外还有抗菌性,抗氧化性,抗肿瘤等生物活性。蚕丝丝素蛋白以其独特的结构,加工时的多功能性,良好的生物相容性,以及易杀菌性、热稳定性、化学可修饰性和可控的降解性,使得丝素蛋白在临床上得到广泛应用。单独使用蚕丝蛋白支架或壳聚糖支架均存在或多或少的不足,其中单独使用丝素蛋白支架存在骨诱导性不强,吸水性能弱,单独使用壳聚糖支架又存在降解性慢、体内不易吸收、细胞黏附性差等问题,所以按照一定比例共混并通过技术加工后得到以复合支架的形式应用于组织工程,彼此弥补了自身的不足,同时具有了有很大的可控性。


背景:蚕丝蛋白/壳聚糖支架具有良好的生物相容性、良好的骨诱导性及良好的降解性等多种特性。
目的:综述蚕丝蛋白/壳聚糖支架的结构和性能及其在骨、软骨及软组织工程再生中的应用。
方法:以“壳聚糖,丝素蛋白,骨组织工程;chitosan,silk protein,bone tissue engineering”为关键词,应用计算机检索PubMed数据库、万方数据库、CNKI数据库1998至2016年的文献,选择与壳聚糖/蚕丝蛋白支架结构、性能及在骨科领域中应用的文献。
结果与结论:蚕丝丝素蛋白/壳聚糖支架兼顾丝素蛋白与壳聚糖的优点,具有良好的生物相容性,能够保证细胞在支架上良好生长,在骨组织工程中应用广泛,不仅对骨缺损修复有很大的帮助,而且在软骨损伤治疗方面也有突出的表现。同时蚕丝丝素蛋白/壳聚糖支架也常被用于软组织工程,对创口愈合、脊髓神经损伤及其他软组织损伤治疗起到重要作用。但由于各方面的原因,目前蚕丝丝素蛋白/壳聚糖支架在临床上使用仍然较少,这将是未来努力的主要方向;基于蚕丝蛋白能用于制备十字韧带和构建工程化髓核,未来丝丝素蛋白/壳聚糖支架可在肌腱韧带损伤、椎间盘组织工程方面进一步研究。

ORCID: 0000-0002-7024-2799(陈跃平)

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

关键词: 生物材料, 骨生物材料, 壳聚糖, 丝素蛋白, 骨组织工程, 支架材料, 广西壮族自治区自然科学基金

Abstract:

BACKGROUND: A silk fibroin/chitosan scaffold has good biocompatibility, osteoinductivity and degradability.
OBJECTIVE: To review the structure, performance and application of the silk fibroin/chitosan scaffold in bone, cartilage and soft tissue engineering regeneration.
METHODS: PubMed, Wanfang, and CNKI databases were retrieved by computer for articles related to the structure, performance and application of the silk fibroin/chitosan scaffold in orthopedics published from 1998 to 2016. The keywords were “chitosan, silk protein, bone tissue engineering” in English and Chinese, respectively.
RESULTS AND CONCLUSION: The silk fibroin/chitosan scaffold is characterized by good biocompatibility, bone inductivity and biodegradability that make cells grow well on the scaffold. The silk fibroin/chitosan scaffold has been widely used in bone tissue engineering, and has a prominent performance in bone defect repair and cartilage injury treatment. Meanwhile, the silk fibroin/chitosan scaffold exerts a crucial role in wound healing as well as in the treatment of spinal nerve injury and other soft tissue injuries. However, the silk fibroin/chitosan scaffold currently is less reported in the clinical use due to various reasons, and it will be the main research direction of future efforts. As is known to us, silk protein can be used to prepare the cruciate ligament and construct tissue-engineered nuclei; therefore, the silk fibroin/chitosan scaffold can be developed in the treatment of tendon ligament injury and intervertebral disc tissue engineering.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Silk, Chitosan, Tissue Engineering

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