中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (25): 4002-4006.doi: 10.3969/j.issn.2095-4344.2015.25.013

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

聚丙交酯/乙交酯胆道支架生物降解及与宿主的相容性

施纯朝1,赵晓飞2,吴晓峰2   

  1. 1东方肝胆外科医院肝外二科,上海市  200438;
    2首都医科大学,北京市  100069
  • 出版日期:2015-06-18 发布日期:2015-06-18
  • 通讯作者: 施纯朝,东方肝胆外科医院肝外二科, 上海市 200438
  • 作者简介:施纯朝,1982年生,男,汉族,福建省人,硕士,医师。

Biodegradability and biocompatibility of polylactide/glycolide biliary stent

Shi Chun-chao1, Zhao Xiao-fei2, Wu Xiao-feng2   

  1. 1Second Department of Liver Surgery, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;
     2Capital Medical University, Beijing 100069, China
  • Online:2015-06-18 Published:2015-06-18
  • Contact: Shi Chun-chao, Second Department of Liver Surgery, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
  • About author:Shi Chun-chao, Master, Physician, Second Department of Liver Surgery, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

摘要:

背景:胆道支架广泛应用于不同的胆道疾病的外科治疗中,但目前使用的胆道支架存在一定的缺陷。
目的:探讨聚丙交酯/乙交酯胆道支架的生物降解性和相容性。
方法:制备聚丙交酯/乙交酯胆道支架,浸入胆汁中,浸泡后1,2,3,4,5周,分别取8个实验支架干燥处理后置于扫描电镜下进行观察。于无菌条件下将实验支架植入大鼠皮下,分别于植入后1,2,3,4,5周,处死2只,将实验支架取出,观察支架外观及实验动物周围肌肉组织情况。观察不同时间的支架大体外观和电镜扫描情况,计算降解率,并了解实验动物肌肉埋植情况和支架情况。
结果与结论:植入后1周支架外形基本保持完整,但质地变软,之后逐渐出现表面粗糙并存在裂痕,支架管壁出现塌陷,植入后5周支架被完全降解。降解前,实验支架经扫描电镜观察呈现出清晰的三维立体网状结构,随着实验时间的不断延长,支架表面和截面腐蚀现象不断加重。经凝胶渗透色谱仪检测发现,植入后1周,支架相对分子质量出现迅速的下降现象,之后渐保持平缓下降状态。植入后2周检测,相对分子质量下降为15 000;植入后4周,支架质量损失约40%;所有实验动物均成活,未出现中毒和过敏以及热源反应等,手术伤口均良好愈合,未发生感染。经组织学观察,植入后5周,支架被完全降解,降解率为100%,周围肌肉组织恢复至正常状态。结果表明,聚丙交酯/乙交酯胆道支架具有良好的体外降解性以及生物相容性。

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

关键词: 生物材料, 材料相容性, 胆道疾病, 支架, 支架材料, 生物相容性材料, 可降解性, 聚丙交酯/乙交酯共聚物, 生物降解, 生物相容性

Abstract:

BACKGROUND: Biliary stent is widely used in different biliary tract surgeries, and there are some defects in the use of biliary stent.
OBJECTIVE: To explore the biodegradability and compatibility of polylactide/glycolide biliary stent.
METHODS: The polylactide/glycolide biliary stent was prepared, and immersed in the bile. At 1, 2, 3, 4 and 5 weeks after soaking, eight experimental stents were dried and placed under the scanning electron microscope. In the sterile condition, the stent was implanted under the rat skin. At 1, 2, 3, 4, and 5 weeks after implantation, two rats were sacrificed. The stent was taken out. The appearance of the stent and muscle structure of experimental animals was observed. The gross appearance of stent was observed at different time by scanning electron microscopy. The rate of degradation was calculated. The muscle structure of experimental animal and the stent were understood.
RESULTS AND CONCLUSION: At 1 week, stent shape remained almost intact, but the texture became soft; surface roughness and cracks gradually appeared; stent wall collapse was visible. The stent was completely degraded at 5 weeks. Before degradation, the stent presented clear three-dimensional reticular structure observed by scanning electron microscopy. With the time extended, the stent surface and section corrosion became worsened. By gel permeation chromatography detection, at 1 week of implantation, the molecular mass of the stent declined rapidly, and then gradually declined. At 2 weeks, the relative molecular mass decreased to 
15 000. At 4 weeks, stent mass loss was approximately 40%. All the experimental animals were survived, no poisoning, allergy or heat source response. The operative wound was healed well, and no infection occurred. By histological observation, at 5 weeks, the stent was completely degraded, with a degradation rate of 100%. Surrounding muscles recovered to a normal state. These results indicate that the polylactide/glycolide biliary stent has good degradation in vitro and biocompatibility.

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Tissue Engineering, Biocompatible Materials, Stents

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