中国组织工程研究

中国组织工程研究 ›› 2025, Vol. 29 ›› Issue (31): 6717-6726.doi: 10.12307/2025.615

• 干细胞相关大数据分析 Stem cell-related big data analysis • 上一篇    下一篇

膜片钳技术10年发展：基于CiteSpace和VOSviewer的可视化分析

郭海珍1，2，丛紫东2，赵玉珂1，2，李晓凤2，于  露1，2，钱舒乐1，2，王润英1，2，杜武勋2   

  1. 1天津中医药大学，天津市  301617；2天津中医药大学第二附属医院，天津市  300150
  • 收稿日期:2024-06-11 接受日期:2024-07-11 出版日期:2025-11-08 发布日期:2025-02-25
  • 通讯作者: 杜武勋，医学博士，教授，主任医师，博士生导师，博士后合作导师。天津中医药大学，天津市 301617；天津中医药大学第二附属医院，天津市 300150
  • 作者简介:郭海珍，女，1995年生，河北省邢台市人，汉族，天津中医药大学在读博士，主要从事心血管疾病的临床与基础、五运六气理论与临床研究。
  • 基金资助:
    国家自然科学基金项目(82004329)，项目负责人：李晓凤；天津市教委科研计划项目(2019SK025)，项目负责人：李晓凤；全国名老中医药专家传承工作室建设项目“杜武勋全国名老中医药专家传承工作室”，项目负责人：杜武勋；天津市名中医传承工作建设项目“杜武勋天津市名中医传承工作室”，项目负责人：杜武勋

Development of patch clamp technology in the past 10 years: visual analysis based on CiteSpace and VOSviewer

Guo Haizhen1, 2, Cong Zidong2, Zhao Yuke1, 2, Li Xiaofeng2, Yu Lu1, 2, Qian Shule1, 2, Wang Runying1, 2, Du Wuxun2   

  1. 1Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; 2Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China
  • Received:2024-06-11 Accepted:2024-07-11 Online:2025-11-08 Published:2025-02-25
  • Contact: Du Wuxun, MD, Professor, Chief physician, Doctoral supervisor, Post-doctoral cooperative supervisor, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China
  • About author:Guo Haizhen, Doctoral candidate, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300150, China
  • Supported by:
    National Natural Science Foundation of China, No. 82004329 (to LXF); Tianjin Municipal Education Commission Scientific Research Plan Project, No. 2019SK025 (to LXF); National Famous Senior TCM Experts Inheritance Studio Construction Project “Du Wuxun National Famous Senior TCM Experts Inheritance Studio” (to DWX); Tianjin Famous TCM Inheritance Work Construction Project “Du Wuxun Tianjin Famous TCM Inheritance Studio” (to DWX)

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摘要：

文题释义：

膜片钳技术：是当前研究细胞膜离子通道的最重要的技术，能记录到pA级电流变化，分辨率高，具有很好的灵活性和灵敏性。
文献计量学：作为大数据统计方法，可用于分析特定研究领域内出版物的产出和状态，挖掘已发表文献的隐藏价值和发展规律，以一种可视化的形式展现出来。

摘要
背景：膜片钳技术作为研究离子通道的“金标准”，已有40多年的发展历史。然而，科研机构的研究内容相对独立，没有对现有研究成果进行系统总结，导致现有研究存在重复性高、创新性弱的现象。因此，急需对膜片钳技术做一个全面的回顾，以明晰现今的研究现状、热点和未来发展方向。
目的：总结近10年膜片钳技术领域的研究现状和发展趋势。
方法：使用Web of Science核心合集数据库收集了2013-2023年关于膜片钳技术的出版物。采用CiteSpace和VOSviewer软件对出版物数量进行量化分析，并分析文献条目网络，包括国家、机构、期刊、作者、关键词、高被引文献和共被引参考文献。
结果与结论：①近10年间，膜片钳技术领域研究已逐步进入稳定发展阶段。②中国和美国是这方面的领先国家，中国科学院是具有核心影响力的机构，《Journal of Neuroscience》是主要出版刊物，Park，Won Sun团队(韩国全北国立大学)和Chu，Li团队(中国河北省心脑血管病中医药防治研究重点实验室)在该领域作出了杰出的贡献，但团队之间的协作与交流较少，尚未形成网络合作模式。③膜片钳技术主要应用在神经系统的电生理特性及其疾病的病理机制方面，是研究人员持续关注的焦点。④在心血管系统电生理特性及其疾病病理机制的研究方面，对原代心肌细胞、诱导多能干细胞衍生的心肌细胞的电生理特性和心房颤动、心脏毒性、心源性猝死和高血压等心血管疾病的病理机制方面的研究，是近几年来研究的热点。⑤在膜片钳技术与其他生物技术的结合应用方面，关注的是与光遗传学、双光子钙成像等技术的交叉融合，将是一个重要的研究方向。⑥在药物筛选及治疗靶点的识别研究方面，尤其对于膜片钳技术和中药复方的研究，将成为未来组分中药研究中的一大助力。

关键词: 膜片钳技术, 全细胞记录, 文献计量学, CiteSpace, VOSviewer, 可视化分析, 神经科学, 电生理, 病理机制, 离子通道

Abstract: BACKGROUND: Patch clamp technique has been developed for more than 40 years as the “gold standard” for the study of ion channels. However, the research content of scientific research institutions is relatively independent, and the existing research results are not systematically summarized, which leads to the phenomenon of high repeatability and weak innovation in the existing research. Therefore, it is urgent to make a comprehensive review of patch clamp technology to clarify the current research status, hot spots, and future development direction.
OBJECTIVE: To summarize the research status and development trend of patch clamp technique in recent 10 years.
METHODS: Publications on patch clamp technology from 2013 to 2023 were collected using the Web of Science core collection database. CiteSpace and VOSviewer software were used to quantify the number of publications and analyze the network of literature entries, including countries, institutions, journals, authors, keywords, highly cited literature, and co-cited references.
RESULTS AND CONCLUSION: (1) In recent 10 years, the research in the field of patch clamp technology has gradually entered a stage of stable development. (2) China and the United States are the leading countries in this regard. The Chinese Academy of Sciences is an institution with core influence. Journal of Neuroscience is the main publication. Park, Won Sun team (Jeonbuk National University) and Chu, Li team (Hebei Key Laboratory of Chinese Medicine Research on Cardio-Cerebrovascular Disease) have made outstanding contributions in this field, but there is less collaboration and communication between the teams and no network cooperation model has been formed. (3) Patch clamp technology is mainly used in the electrophysiological characteristics of the nervous system and the pathological mechanism of the disease, which is the focus of researchers’ continuous attention. (4) In the study of electrophysiological characteristics of cardiovascular system and its pathological mechanism, the electrophysiological characteristics of primary cardiomyocytes and induced pluripotent stem cell-derived cardiomyocytes and the pathological mechanism of atrial fibrillation, cardiotoxicity, sudden cardiac death, hypertension and other cardiovascular diseases have been the focus of research in recent years. (5) In the application of patch clamp technology combined with other biotechnology, it will be an important research direction to focus on the cross fusion with optogenetics, two-photon calcium imaging and other technologies. (6) In the research of drug screening and identification of therapeutic targets, especially the research of patch clamp technology and traditional Chinese medicine compound, it will become a great help in the future research of component traditional Chinese medicine. 

Key words: ">patch clamp technique, whole cell recording, bibliometrics, CiteSpace, VOSviewer, visual analysis, neuroscience, electrophysiology, pathological mechanism, ion channel

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