中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (10): 1586-1592.doi: 10.3969/j.issn.2095-4344.0720

• 生物材料综述 biomaterial review • 上一篇    下一篇

β折叠型自组装短肽水凝胶特性及在神经组织工程中的应用前景

卫  巍1,刘燕飞1,何  洋2,张  玲1
  

  1. 1遵义医学院附属医院贵州省细胞工程重点实验室,贵州省遵义市  563000;2遵义医学院,贵州省遵义市  563003
  • 收稿日期:2018-02-01 出版日期:2018-04-08 发布日期:2018-04-08
  • 通讯作者: 刘燕飞,博士,副教授,硕士生导师,遵义医学院附属医院贵州省细胞工程重点实验室,贵州省遵义市 563003
  • 作者简介:卫巍,男,1992年生,湖北省十堰市人,汉族,遵义医学院在读硕士,主要从事自组装短肽设计、特性及组织工程方向的研究。
  • 基金资助:
    贵州省联合资金资助项目(黔科合J字LKZ[2013]01号);贵州省教育厅自然科学研究项目(黔教合KY字[2015]418)
     

The application of beta-sheet self-assembling peptide hydrogels in neural tissue engineering

Wei Wei1, Liu Yan-fei1, He Yang2, Zhang Ling1
  

  1. 1Key Laboratory of Cell Engineering in Guizhou Province, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China; 2Zunyi Medical University, Zunyi 563003, Guizhou Province, China
  • Received:2018-02-01 Online:2018-04-08 Published:2018-04-08
  • Contact: Liu Yan-fei, M.D., Associate professor, Master’s supervisor, Key Laboratory of Cell Engineering in Guizhou Province, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • About author:Wei Wei, Master candidate, Key Laboratory of Cell Engineering in Guizhou Province, the Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • Supported by:
    the Key Project of Guizhou Provincial Science and Technology Department, No. LKZ[2013]01; the Natural Science Research Project of Education Department of Guizhou Province, No. [2015]418

摘要:

文章快速阅读:

 

文题释义:
分子自组装:是自然界中普遍存在的现象,它无需外界干预而自发形成稳定结构。自1993年Zhang等首次报道了在Zuotin蛋白中发现自组装短肽EAK16-Ⅱ后,一系列自组装短肽材料相继被发现和设计合成,其中最具代表性自组装短肽为RADA16-Ⅰ。这类短肽材料由相互交替的亲水性和疏水性氨基酸序列组成,在一定条件刺激下,能够自发且有规律的通过非共价键作用形成稳定的β折叠结构,再进一步堆叠为纳米纤维,最终形成含水量超过99%的水凝胶支架。
水凝胶:凡是水溶性或亲水性的高分子,通过一定的化学交联或物理交联都可形成水凝胶。自组装短肽材料能够依靠物理性的非共价键作用介导,自组装形成纳米纤维水凝胶,自组装短肽水凝胶材料具有以下优势:①具有良好的生物相容性,无细胞毒性,低免疫原性,不影响组织正常愈合;②能形成稳定的三维支架,该支架结构具有适度的机械张力和通透性;③自组装短肽水凝胶降解产物为氨基酸,不引起组织炎症反应,可被吸收再利用。因此,自组装短肽作为一种理想生物支架材料在细胞三维培养、组织工程、再生医学及药物缓释等领域的应用有着巨大的潜力。
 
摘要
背景:自组装短肽具有能够优化其三维支架的组分和结构特性,可作为新型组织工程支架材料应用于生物医药领域。
目的:概述β折叠型自组装短肽的生物学特性及其在神经组织工程中的研究进展。
方法:以“β折叠,自组装短肽,水凝胶,神经干细胞,神经组织工程;β sheet,self-assembling peptide,hydrogel,neural stem cell,neural tissue engineering”为检索词,检索PubMed、CNKI、万方数据库2000至2017年发表的相关文献。
结果与结论:β折叠型自组装短肽可模拟细胞外基质结构,促进神经干细胞的黏附、迁移、分化和突触发生,引导神经细胞生长和轴突连接。在神经组织工程中,自组装短肽RADA16、LDLK12、QL6及其衍生出的功能化序列已被证实具有良好的实用性。细胞培养与动物实验显示,β折叠型自组装短肽可促进干细胞的神经元分化,在神经退行性疾病、脊髓损伤、脑损伤及外周神经损伤中有很好的应用前景。

关键词: β折叠, 自组装短肽, 水凝胶, 神经干细胞, 神经组织工程, 生物材料

Abstract:

BACKGROUND: Self-assembling peptides, which allow for an optimal control over the scaffold structure and composition, are described as the innovative tissue-engineered scaffolds in the field of biological medicine.
OBJECTIVE: To summarize the biological features of β-sheet self-assembling peptides and current research progress in the neural tissue engineering.
METHODS: We took “β sheet, self-assembling peptide, hydrogel, neural stem cell, neural tissue engineering” as the key words in Chinese and English, respectively, to retrieve the related literatures (2000-2017) from PubMed, CNKI, WanFang databases based on internet search.
RESULTS AND CONCLUSION: β-sheet self-assembling peptide, designed to function as an engineered mimic of the extra-cellular matrix (ECM), may be used with as a medium to direct growth and axonal connection, promoting neural stem cells adhesion, migration, division, outgrowth, neurogenesis and synaptogenesis. The self-assembling peptides RADA16, LDLK12, QL6 and their functionalized sequences have been proven to be of good utility. As the excellent performance of inducing peripheral nerve regeneration, mediating spinal cord injury and attenuating neuroinflammation, β-sheet self-assembling peptides have promising applications in the neural tissue engineering.

Key words: Protein Folding, Hydrogel, Neural Stem Cells, Tissue Engineering

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