中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (48): 9030-9033.doi: 10.3969/j.issn.2095-4344.2012.48.019

• 脊柱损伤基础实验 basic experiments of spinal injury • 上一篇    下一篇

正常与退变髓核细胞的变形能力

任龙韬1，卫陈刚2，牛建鹏1，林树忠1   

  1. 1太原市中心医院骨科，山西省太原市 030009
    2山西医科大学研究生学院，山西省太原市 030001
  • 收稿日期:2012-02-02 修回日期:2012-03-27 出版日期:2012-11-25 发布日期:2013-03-14
  • 作者简介:任龙韬，男，1958年生，山西省太原市人，汉族，1982年山西医科大学毕业，主任医师，教授，硕士生导师，主要从事骨关节疾病和生物力学研究。 renlongtaoguke@163.com

Distortion capability of normal and degenerative nucleus pulposus cells

Ren Long-tao1, Wei Chen-gang2, Niu Jian-peng1, Lin Shu-zhong1   

  1. 1Department of Orthopedics, Taiyuan City Centre Hospital, Taiyuan 030009, China
    2Graduate Department of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Received:2012-02-02 Revised:2012-03-27 Online:2012-11-25 Published:2013-03-14
  • About author:Ren Long-tao, Chief physician, Professor, Master’s supervisor, Department of Orthopedics, Taiyuan City Centre Hospital, Taiyuan 030009, China renlongtaoguke@ 163.com

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529

被引次数

7

摘要：

背景：细胞的变形能力在较大程度上能反映出细胞结构与功能的改变，目前对髓核细胞在细胞力学方面的研究较少。
目的：分析正常和退变髓核细胞的变形能力。
方法：正常髓核组织和退变髓核组织分别取材于3例脊柱侧弯患者和3例椎间盘突出患者术中取出的废弃髓核组织，用胰蛋白酶和Ⅱ型胶原酶分离提取细胞，行原代培养，通过微管加载装置适宜负压下测量细胞在第4秒时吸入微管的长度，并行统计学处理。
结果与结论：正常髓核细胞和退变髓核细胞所用的负压分别为(411.31±27.93) Pa，(434.24±46.26) Pa，差异有显著性意义(P < 0.05)。在4 s时吸入长度分别为(2.20±0.92) μm，(2.48±0.71) μm，差异无显著性意义(P > 0.05)。结果可见在规定时间的情况下，吸入微管的长度基本相同时，退变髓核细胞所需要的负压较正常髓核细胞大，说明退变髓核细胞的变形能力比正常髓核细胞差。

关键词: 髓核细胞, 退变, 椎间盘, 细胞变形能力, 细胞力学

Abstract:

BACKGROUND: The distortion capability of cells can reflect the changes of cell structure and function. To date, the studies on the cell mechanics of nucleus pulposus are rare.
OBJECTIVE: To analyze the distortion capability of normal and degenerative nucleus pulposus in vitro.
METHODS: The normal and degenerative nucleus pulposus cells were respectively obtained from discarded nucleus pulposus tissue of 3 scoliosis patients and 3 intervertebral disc hernitaion patients. Pancreatin and collagenase type Ⅱwere used to isolate the nucleus pulposus tissue and performed with primary culture. The micropipette aspiration test was used to measure the length of nucleus pulposus cells aspirated into micropipette at 4 seconds under suitable negative pressure, and the statistical analysis was performed.
RESULTS AND CONCLUSION: The negative pressure of the normal and degenerative nucleus pulposus cells were (411.31±27.93) Pa and (434.24±46.26) Pa respectively, and the difference was significant (P < 0.05). Within 4 seconds, the lengths of the normal and degenerative nucleus pulposus cells aspirated into micropipette were (2.20±0.92) μm and (2.48±0.71) μm, their differences were not significant (P > 0.05). With the certain time and the same length of the normal and degenerative nucleus pulposus cells aspirated into micropipette, the degenerative nucleus pulposus cells needed greater negative pressure than normal nucleus pulposus cells. It indicates that the distortion capability of degenerative nucleus pulposus cells is worse than that of normal nucleus pulposus cells.

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