中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8615-8619.doi: 10.3969/j.issn.2095-4344.2012.46.013

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

玻璃化冷冻法保存关节软骨

宋洪强1，玄燕华2，吴雅迪1，亓建洪1   

  1. 1泰山医学院运动医学研究所，山东省泰安市 271000
    2泰山医学院附属医院，山东省泰安市 271000
  • 收稿日期:2012-03-27 修回日期:2012-04-24 出版日期:2012-11-11 发布日期:2012-11-11
  • 作者简介:宋洪强★，男，1977年生，山东省泰安市人， 2006年泰山医学院毕业，硕士，讲师，主要从事关节软骨损伤与修复研究。 shq2369@163.com

Vitrification method for cryopreservation of the articular cartilage

Song Hong-qiang1, Xuan Yan-hua2, Wu Ya-di1, Qi Jian-hong1   

  1. Institute of Sports Medicine, Taishan Medical College, Taian 271000, Shandong Province, China
    2Affiliated Hospital of Taishan Medical College, Taian 271000, Shandong Province, China
  • Received:2012-03-27 Revised:2012-04-24 Online:2012-11-11 Published:2012-11-11
  • About author:Song Hong-qiang★, Master, Lecturer, Institute of Sports Medicine, Taishan Medical College, Taian 271000, Shandong Province, China shq2369@163.com

PDF

525

被引次数

7

摘要：

背景：同种异体骨软骨移植技术是治疗关节软骨缺损有效的方法之一，但由于移植物保存方法不理想，明显制约着该技术的临床应用。
目的：探讨玻璃化冷冻法保存关节软骨组织的可行性和优越性。
方法：切取成年猪骨软骨，制成约5 mm×6 mm(直径×长度)大小的圆柱形骨软骨块。以新鲜软骨组为对照，分别采用0.5 mol/L甘油 、1 mol/L二甲基亚砜、1 mol/L玻璃化溶液3种方法预处理软骨块，再行冷冻法保存软骨块8周，采用组织化学染色、免疫荧光染色观察并比较软骨细胞活性的变化。
结果与结论：玻璃化溶液预处理组的关节软骨细胞存活率达到74.5%，明显高于甘油和二甲基亚砜预处理组，软骨基质成分仅少量丢失。3种方法相比较，玻璃化溶液预处理后慢速梯度降温冷冻保存法可以明显提高冻存关节软骨组织的活性。

关键词: 玻璃化, 梯度降温, 关节软骨, 冷冻保存, 细胞活性

Abstract:

BACKGROUND: Articular cartilage allograft transplantation is one of effective methods for treatment of articular cartilage defects. However, the short preservation time of osteochondral allograft in vitro limits its clinical application
OBJECTIVE: To discuss the feasibility and superiority of vitrification method on the viability of chondrocytes.
METHODS: Osteochondral tissues were isolated from adult pigs, and cut into approximately 5 mm×6 mm (diameter×length) cylindrical osteochondral blocks. Fresh cartilage was taken as control. The osteochondral blocks was pretreated with 0.5 mol/L glycerol, 1 mol/L dimethyl sulfoxide, and 1 mol/L vitrification solution, respectively, and then cryopreserved for 8 weeks. Histochemistry staining and immunofluorescence staining were performed to observe and compare the changes in chondrocyte viability.
RESULTS AND CONCLUSION: The viability of chondrocytes was 74.5% in the vitrification pretreatment group, significantly higher than the glycerol and dimethyl sulfoxide pretreatment groups. In addition, there was a small loss of cartilage matrix in the vitrification pretreatment group. In summary, the vitrification-cryopreservation method can greatly improve the viability of frozen chondrocytes.

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