中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5315-5320.doi: 10.3969/j.issn.2095-4344.2012.28.037

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    

肺表面活性物质相关蛋白A基因多态性与新疆蒙古族慢性阻塞性 肺疾病患者的发病

关 键1, 2，徐永健1，许西琳2，骆树新2，季宏志2   

  1. 1华中科技大学同济医学院附属同济医院呼吸与危重症医学科，湖北省武汉市 430030；
    2 石河子大学医学院第一附属医院呼吸科，新疆维吾尔自治区石河子市 832008
  • 收稿日期:2012-03-02 修回日期:2012-05-11 出版日期:2012-07-08 发布日期:2012-07-08
  • 作者简介:关键☆，男，1968年生，汉族，河南省柘城县人，华中科技大学同济医学院在读博士，副教授，主要从事慢性阻塞性肺疾病的研究。 guanjian6@163.com

Relationship between surfactant protein A polymorphism and chronic obstructive pulmonary disease in Xinjiang Mongolian population

Guan Jian1, 2, Xu Yong-jian1, Xu Xi-lin2, Luo Shu-xin2, Ji Hong-zhi2   

  1. 1Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China;
    2Department of Respiratory Medicine, the First Affiliated Hospital of Medical College, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China
  • Received:2012-03-02 Revised:2012-05-11 Online:2012-07-08 Published:2012-07-08
  • About author:Guang Jian☆, Studying for doctorate, Associate professor, Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China; Department of Respiratory Medicine, the First Affiliated Hospital of Medical College, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China guanjian6@163.com

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摘要：

背景：目前慢性阻塞性肺疾病的遗传易感性机制未完全阐明。肺表面活性物质相关蛋白A可能在其易感性机制中发挥重要作用，且可能有种族差异。
目的：探讨肺表面活性物质相关蛋白A基因位点aa62、aa219多态性与新疆蒙古族慢性阻塞性肺疾病易感性的关系。
方法：采用病例-对照研究设计，纳入新疆蒙古族吸烟慢性阻塞性肺疾病患者119例(病例组)和吸烟健康体检者116例(对照组)为研究对象。采用Taqman探针标记荧光定量PCR法检测肺表面活性物质相关蛋白A基因的aa62(CCA/CCG，rs1136451)和aa219(CGG/TGG，rs4253527)位点的单核苷酸多态性，获得各基因型和等位基因的分布情况。
结果与结论：在aa62位点，两组的基因型频率(AA，AG、GG)及等位基因频率(A、G)比较差异均有显著性意义 (χ2=7.164，P=0.028；χ2=2.239，P=0.019)；且基因型频率在肺功能Ⅱ级与Ⅲ级慢性阻塞性肺疾病患者间差异有显著性意义(χ2=8.721，P=0.013)。在aa219位点，两组基因型频率(CC，CT)及等位基因频率(C、T)比较差异均无显著性意义(χ2=0.367，P=0.545；χ2=0.332，P=0.565)。提示肺表面活性物质相关蛋白A基因aa62位点多态性可能与新疆蒙古族吸烟慢性阻塞性肺疾病的易感性相关。

关键词: 表面活性物质相关蛋白A, 慢性阻塞性肺疾病, 基因多态性, 易感性, 吸烟

Abstract:

BACKGROUND: At present, the genetic pathogenesis of chronic obstructive pulmonary disease has not been fully understood yet. Surfactant protein A may play an important role in the pathogenesis of genetic susceptibility in chronic obstructive pulmonary disease, and there may be a racial difference.
OBJECTIVE: To investigate the relationship between surfactant protein A polymorphism and chronic obstructive pulmonary disease in Xinjiang Mongolian population.
METHODS: The case-control study was used in this study. DNAs were extracted from the peripheral blood of 119 smokers with chronic obstructive pulmonary disease and 116 healthy smokers from Mongolian population. Polymorphisms of rs1136451 and rs4253527 were determined by the Taqman PCR method.
RESULTS AND CONCLUSION: Genotypes frequencies were different between the chronic obstructive pulmonary disease and normal smokers for aa62 (χ2=7.164, P=0.028). There were also significant differences in allele genotype frequencies to the chronic obstructive pulmonary disease smokers (χ2=2.239, P=0.019). There were differences in genotype frequency stage II and stage Ⅲ of chronic obstructive pulmonary disease (χ2=8.721, P=0.013). Genotypes frequencies were not different between the chronic obstructive pulmonary disease and normal smokers for aa219 (χ2=0.367, P=0.545); there were also no differences in allele genotype (χ2=0.332, P=0.565). The polymorphism of aa62 (CCA/CCG, rs1136451) in surfactant protein A may be associated with the susceptibility to chronic obstructive pulmonary disease in Xinjiang Mongolian population.

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