中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5287-5290.doi: 10.3969/j.issn.2095-4344.2012.28.032

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

钙网蛋白在心肌组织细胞氧化应激与缺血损伤构建中的表达与应用

李雷勇1，田岳凤2，袁 叶2，王 军2，张斌仁2，王 旭2   

  1. 1山西医科大学第二医院检验科，山西省太原市 030001；
    2山西中医学院针灸推拿学院，山西省太原市 030024
  • 收稿日期:2011-09-21 修回日期:2011-10-24 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 田岳凤，教授，山西中医学院针灸推拿学院，山西省太原市 030024 tyfsx@163.com
  • 作者简介:李雷勇，男，1963年生，江苏省徐州市人，汉族，1987年山西大学毕业，副主任技师，主要从事生化及分子生物学检测技术的研究。

Expression and application of calreticulin in the construction of cardiac histiocyte oxidative stress and ischemic injury

Li Lei-yong1, Tian Yue-feng2, Yuan Ye2, Wang Jun2, Zhang Bin-ren2, Wang Xu2   

  1. 1Laboratory Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China;
    2School of Acupuncture and Moxibustion, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, Shanxi Province, China
  • Received:2011-09-21 Revised:2011-10-24 Online:2012-07-08 Published:2012-07-08
  • Contact: Tian Yue-feng, Professor, School of Acupuncture and Moxibustion, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, Shanxi Province, China tyfsx@163.com
  • About author:Li Lei-yong, Associate chief technician, Laboratory Department, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China

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432

被引次数

1

摘要：

背景：生物体内钙网蛋白具有调节细胞凋亡、应激、心血管炎症反应等多种生理和病理生理过程的功能。
目的：分析钙网蛋白在缺血再灌注损伤中的表达与应用。
方法：由作者检索1996/2010 PubMed数据库与中国知网数据库有关钙网蛋白结构、功能及在自身免疫、心脏发育中的作用、在缺血缺氧过程中表达的变化等方面的文献后，对钙网蛋白在氧化应激与缺血损伤中的表达变化进行了实验观察。
结果与结论：钙网蛋白通过协助蛋白质正确折叠和维持细胞内Ca2+稳态而参与调节细胞凋亡、黏附、类固醇敏感基因表达等，在心脏发生、发育和病理变化中发挥着重要作用。作者应用大鼠心肌缺血再灌注损伤模型，通过酶联免疫吸附试验、免疫组织化学及RT-PCR 3种不同的检测方法，观察了钙网蛋白在心肌缺血再灌注损伤中的表达，结果显示缺血再灌注损伤诱导钙网蛋白高表达，进而激活内质网凋亡信号途径，诱导细胞损伤，加重心肌损伤。

关键词: 钙网蛋白, 心肌缺血-再灌注损伤, 检测方法, 蛋白表达, 心脏

Abstract:

BACKGROUND: Calreticulin regulates many physiological and pathophysiological processes in vivo, such as cell apoptosis, stress and cardiovascular inflammatory response.
OBJECTIVE: To analyze the expression and application of calreticulin in ischemia-reperfusion injury.
METHODS: An online search of PubMed and CNKI databases was performed for relevant articles published from 1996 to 2010. Articles on the structure and function of calreticulin, the role of calreticulin in autoimmunization and cardiac development, the expression change of calreticulin in the ischemia-reperfusion process were included. On that basis, the expression changes of calreticulin in oxidative stress and ischemia-reperfusion injury were observed by experiment.
RESULTS AND CONCLUSION: Calreticulin plays an important role in the genesis, development and pathological changes of hearts by assisting protein folding correctly, maintaining the intracellular Ca2+ homeostasis as well as involving in the regulation of apoptosis, adhesion and the expression of steroid sensitive genes. Calreticulin expression in myocardial ischemia-reperfusion injury was detected by enzyme-linked immunosorbent assay, immunohistochemistry and RT-PCR using myocardial ischemia-reperfusion injury model in rats. The results indicate that calreticulin expression is increased after myocardial ischemia-reperfusion injury, and then the apoptosis signal pathway in endoplasmic reticulum is activated, leading to cell injury and aggravate the myocardial injury.

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