中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (14): 2524-2529.doi: 10.3969/j.issn.1673-8225.2012.14.011

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

联合培养体系中对骨髓间充质干细胞Bmi-1表达的影响**☆

杨瑞年，刘  流，王福科，赵德萍   

  1. 昆明医学院第一附属医院头颈外科，云南省昆明市   650032
  • 收稿日期:2011-12-27 修回日期:2012-01-22 出版日期:2012-04-01 发布日期:2012-04-01
  • 通讯作者: 刘流，教授，博士生导师，昆明医学院第一附属医院头颈外科，云南省昆明市 650032 liuliu3939@126.com
  • 作者简介:杨瑞年☆，男，1981年生，云南省昆明市人，纳西族，昆明医学院第一附属医院头颈外科在读博士，医师，主要从事整形外科及组织工程修复的研究。13888425351@163.com
  • 基金资助:

    国家自然科学基金资助项目(30960388；81160239)。

Effects of co-culture system on Bmi-1 expression in bone marrow mesenchymal stem cells

Yang Rui-nian, Liu Liu, Wang Fu-ke, Zhao De-ping   

  1. Department of Head and Neck Surgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • Received:2011-12-27 Revised:2012-01-22 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Liu Liu, Professor, Doctoral supervisor, Department of Head and Neck Surgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China liuliu3939@126.com
  • About author:Yang Rui-nian☆, Studying for doctorate, Physician, Department of Head and Neck Surgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032,Yunnan Province, China 13888425351@ 163.com
  • Supported by:

    the National Natural Science Foundation of China, No.30960388*; 81160239*

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摘要：

背景：血管内皮细胞能够促进骨髓间充质干细胞向成骨方向转变，并为干细胞的生长增殖提供营养支持。
目的：观察人脐静脉血管内皮细胞在联合培养体系中对人骨髓间充质干细胞的形态、生长、细胞分化及其Bmi-1基因表达的影响。
方法：在建立细胞联合培养体系基础上，设立单纯骨髓间充质干细胞培养组、骨髓间充质干细胞与脐静脉血管内皮细胞联合培养组，分别于第4，6，8，10天在相差显微镜下观察形态变化、细胞计数绘制生长曲线并采用实时荧光定量PCR检测单独培养的人骨髓间充质干细胞组及联合培养组中人骨髓间充质干细胞的Bmi-1基因表达情况。
结果与结论：在各时间点与单纯骨髓间充质干细胞培养组相比较，联合培养组中干细胞的形态呈多样化，后期部分细胞之间出现了连接，成骨分化明显。联合培养组的细胞数量以及Bmi-1表达量各时间点均显著高于单纯骨髓间充质干细胞培养组。联合培养相容性良好。提示脐静脉内皮细胞对体外联合培养体系中骨髓间充质干细胞具有促进增殖的作用；能促进骨髓间充质干细胞Bmi-1基因的表达，对细胞衰老和增殖能力有显著影响。

关键词: 血管内皮细胞, 骨髓间充质干细胞, 脐静脉血管内皮细胞, Bmi-1, 联合培养

Abstract:

BACKGROUND: Vascular endothelial cells can promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and provide nutritional support for growth and proliferation of BMSCs.
OBJECTIVE: To investigate the effects of human umbilical vein vascular endothelial cells (hUVECs) in a co-culture system on morphology, growth and differentiation of human BMSCs, and Bmi-1 gene expression in cells.
METHODS: Based on the co-culture system of cells, a simple culture group (hBMSCs group) and a combined culture group (BMSCs + hUVECs group) were established. On days 4, 6, 8, 10, cell morphology was observed and cells were counted for drawing cell growth curves; in addition, Bmi-1 gene expression in cells was detected in each group using real-time quantitative fluorescent PCR.
RESULTS AND CONCLUSION: At each time point, compared with hBMSCs group, cells exhibited multiple morphological changes, some cells connected together in the latter stage, and osteogenic differentiation was more obvious in the BMSCs + hUVECs group. At each time point, cell number and Bmi-1 expression in the BMSCs + hUVECs group were significantly greater than in the BMSCs group. The BMSCs + hUVECs group showed a better compatibility compared with the hBMSCs group. These findings suggest that hUVECs promote the proliferation of hBMSCs in the co-culture system, and increase Bmi-1 expression in hBMSCs, thereby affecting aging and proliferative capacity of hBMSCs.

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