中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9579-9582.doi: 10.3969/j.issn.1673-8225.2011.51.019

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

生物相容性Fe3O4@SiO2纳米粒子的合成及性能

孙鹏飞1，颉克蓉1，张锋伟2，焦宗宪3，曹向荣1   

  1. 1兰州大学第二医院，甘肃省兰州市 730030；兰州大学，2化学化工学院，3基础医学院，甘肃省兰州市  730000
  • 收稿日期:2011-05-17 修回日期:2011-07-27 出版日期:2011-12-17 发布日期:2011-12-17
  • 作者简介:孙鹏飞☆，男，1973年生，甘肃省武山县人，汉族，2007年解放军第二军医大学毕业，博士，副主任医师，副教授，硕士生导师，主要从事肿瘤影像诊断与放射治疗研究。 sunpengfeiyy@msn.com
  • 基金资助:

    兰州大学中央高校基本科研业务费专项资金资助(lzujbky-2009-154)。

Preparation and characterization of biocompatible Fe3O4@SiO2 nanoparticals

Sun Peng-fei1, Xie Ke-rong1, Zhang Feng-wei2, Jiao Zong-xian3, Cao Xiang-rong1   

  1. 1Lanzhou University Second Hospital, Lanzhou  730030, Gansu Province, China; 2College of Chemistry & Chemical Engineering, Lanzhou University, Lanzhou  730000, Gansu Province, China; 3College of Basic Medical Science, Lanzhou University, Lanzhou  730000, Gansu Province, China
  • Received:2011-05-17 Revised:2011-07-27 Online:2011-12-17 Published:2011-12-17
  • About author:Sun Peng-fei☆, Doctor, Associate chief physician, Associate professor, Master’s supervisor, Lanzhou University Second Hospital, Lanzhou 730030, Gansu Province, China sunpengfeiyy@ msn.com
  • Supported by:

    the Fundamental Research Funds for the Central Universities in Lanzhou University, No. lzujbky-2009-154*

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摘要：

背景：Fe3O4纳米粒子具有良好的磁学特性，SiO2具有良好的生物相容性，Fe3O4@SiO2复合纳米粒子有望成为靶向治疗的载体。
目的：采用反相微乳液法合成生物相容性的Fe3O4@SiO2纳米粒子。
方法：首先，以FeCl3•6H2O、FeCl2•4H2O、油酸、氨水等为原料，采用一壶化学共沉淀法合成油酸修饰的疏水性Fe3O4纳米粒子。随后，将油酸包裹的Fe3O4纳米粒子分散于环己烷中，然后将Triton-X100、正己醇及水在搅拌条件下加入到上述溶液，形成稳定的反相微乳液；在反相微乳液中，以氨水为催化剂，使正硅酸四乙酯水解、缩合，从而获得Fe3O4@SiO2复合纳米粒子。
结果与结论：①透射电镜、X射线衍射显示：采用一壶化学沉淀法合成的Fe3O4具有尖晶石结构，平均粒径约为3.5 nm；微乳液法能将SiO2成功包覆于Fe3O4表面，形成平均粒径为40 nm的均一Fe3O4@SiO2复合纳米粒子。②磁性能分析显示：Fe3O4纳米粒子包裹后饱和磁化强度下降，但包裹前后矫顽力趋于零，均显示超顺磁性。③MTT结果显示纳米粒子与人脐静脉细胞融合细胞(EA.hy926)共培养24 h时Fe3O4@SiO2组吸光度高于对照组(P < 0.05)；细胞培养48，72 h，两组比较差异无显著性意义(P > 0.05)。结果表明经反相微乳液法合成的Fe3O4@SiO2纳米粒子是一种优良的生物材料，其具有稳定、易分散及超顺磁性等特性。

关键词: 化学共沉淀法, 二氧化硅, 四氧化三铁, 纳米粒子, 微乳液法

Abstract:

BACKGROUND: Fe3O4 nanoparticles have good magnetic properties, SiO2 has good biocompatibility, and Fe3O4@SiO2 composite nanoparticles are expected to become the carrier of targeted therapy.
OBJECTIVE: To prepare the biocompatible Fe3O4@SiO2 nanoparticles via a reverse microemulsion.
METHODS: Firstly, hydrophobicity Fe3O4 nanoparticle modified oleic acid were prepared via a one-pot chemical coprecipitation with FeCl3•6H2O, FeCl2•4H2O, oleic acid and NH3•H2O. Subsequently, Fe3O4 nanoparticles stabilized with oleic acid were dispersed in cyclohexane, and then Triton-X100, hexylalcohol and H2O were added into the above solution and stirred to form stable reverse microemulsion; In the reverse microemulsion, using ammonia solution as a catalyst, tetraethyl orthosilicate (TEOS) was hydrolyzed and condensed to form Fe3O4@SiO2 compound nanoparticles.
RESULTS AND CONCLUSION: ①The structure of Fe3O4 nanopartides prepared by a one-pot chemical coprecipitation was spinel, having a mean diameter of 3.5 nm; Through microemulsion methods, SiO2 were coated successfully on the surface of Fe3O4 nanopartides, and the uniform Fe3O4@SiO2 nanoparticles with a mean diameter of 40 nm were obtained. ②Although the saturation magnetization decreased after Fe3O4 nanopartides being coated, the coercivity of magnetic nanoparticles was near zero before and after encapsulation, revealing Fe3O4 and Fe3O4@SiO2 nanoparticles are of superparamagnetic properties.③After EA. hy926 cells were cultured accompanied with Fe3O4@SiO2 nanoparticals, the absorbance value of Fe3O4@SiO2 nanoparticles was markedly higher than that of control group (P < 0.05); At 48 and 72 hours of cell culture, no significant difference was found in the absorbance value between the two group (P > 0.05). The results showed Fe3O4@SiO2 compound nanoparticle using a reverse microemulsion is a kind of excellent biomaterial because of its stable, dispersive and superparamagnetic properties.

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