中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9544-9548.doi: 10.3969/j.issn.1673-8225.2011.51.011

• 复合支架材料 composite scaffold materials • 上一篇    下一篇

骨髓间充质干细胞-聚乙醇酸支架复合体种植动物皮下构建组织工程化小口径血管

高国亮，宋剑非，王海永，郑  民，王  玮，姜亦瑶，杜振宗   

  1. 桂林医学院附属医院胸心外科，广西壮族自治区桂林市  541001
  • 收稿日期:2011-04-20 修回日期:2011-06-29 出版日期:2011-12-17 发布日期:2011-12-17
  • 通讯作者: 杜振宗，博士，副教授，副主任医师，硕士生导师，桂林医学院附属医院胸心外科，广西壮族自治区桂林市 541001
  • 作者简介:高国亮★，男，1984年生，山西省太原市人，汉族，桂林医学院在读硕士，主要从事干细胞和组织工程的研究。
  • 基金资助:

    广西壮族自治区卫生厅立项课题(Z2007199)，课题名称：骨髓间充质干细胞诱导血管平滑肌细胞和组织相容性分析；广西壮族自治区卫生厅立项课题(Z2008290)，课题名称：诱导骨髓间充质干细胞向气管软骨细胞分化的实验研究。

Subcutaneous implantation of bone marrow mesenchymal stem cells-polyglycolic acid scaffold complex to construct small diameter tissue-engineered blood vessels

Gao Guo-liang, Song Jian-fei, Wang Hai-yong, Zheng Min, Wang Wei, Jiang Yi-yao, Du Zhen-zong   

  1. Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin  541001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-20 Revised:2011-06-29 Online:2011-12-17 Published:2011-12-17
  • Contact: Du Zhen-zong, Doctor, Associate professor, Associate chief physician, Master’s supervisor, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China duzhenzong@ sina.com
  • About author:Gao Guo-liang★, Studying for master’s degree, Department of Cardiothoracic Surgery, Affiliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Autonomous Region, China 26313597@qq.com
  • Supported by:

    the Healthy Department of Guangxi Zhuang Autonomous Region, No. Z2007199*; Z2008290*

PDF

412

被引次数

2

摘要：

背景：课题组的前期工作已证实骨髓间充质干细胞可以诱导分化为血管内皮细胞和血管平滑肌细胞，并证实所诱导的细胞和胶原包埋的聚乙醇酸支架具有良好的组织相容性。
目的：探讨利用动物皮下作为生物反应器构建小口径组织工程化血管的可行性。
方法：骨髓间充质干细胞诱导分化为血管平滑肌样细胞和血管内皮样细胞，分层种植于胶原包埋聚乙醇酸支架表面，然后将细胞-支架复合体种植于动物皮下，构建小口径组织工程化血管。
结果与结论：人工血管组织学观察见管壁结构清晰，其大体结构和天然血管相似，可承受26.6 kPa的血管腔内压力不破裂。皮下培养8周免疫荧光观察Brdu标记的部分细胞核呈现明亮的黄绿色荧光。结果说明利用动物的皮下作为生物反应器，采用静态培养的方式构建小口径组织工程化血管是可行的。

关键词: 聚乙醇酸, 骨髓间充质干细胞, 平滑肌细胞, 血管内皮细胞, 胶原, 组织工程化血管, 生物反应器, 细胞种植

Abstract:

BACKGROUND: Our former studies have shown that bone marrow mesenchymal stem cells (BMMSCs) can be induced differentiation to vascular smooth muscle-like cells (VSMLCs) and vascular endothelium-like cells (VELCs), which are compatible with collagen-embedded polyglycolic acid scaffolds.
OBJECTIVE: To investigate the possibility of constructing small diameter tissue-engineered blood vessels via subcutaneous implantation.
METHODS: The cells-scaffold complex was produced by separately seeding VSMLCs and VELCs derived from BMMSCs on polyglycolic acid collagen scaffolds. The two layers were separated by ECMgel. The cells-scaffold complex was subcutaneous implanted into small diameter tissue-engineered blood vessels.
RESULTS AND CONCLUSION: Histological analysis of the small diameter tissue-engineered blood vessel walls revealed a typical artery structure, which was similar to natural vessels. The tissue-engineered blood vessels were not broken down under a force of 26.6 kPa. Eight weeks after implantation, the Brdu-labeled seed cells were found in the three layers of the vessel walls. The results revealed that the subcutaneous tissue was a good bioreactor to construct small diameter tissue-engineered blood vessels.

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