中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (49): 9231-9234.doi: 10.3969/j.issn.1673-8225.2011.49.026

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

癌-睾丸抗原基因在神经胶质瘤干细胞中的表达

唐景峰1，肖绍文2，黄永旺1，杨保华1，杜贻庆1   

  1. 1桂林医学院附属医院神经外科，广西壮族自治区桂林市  541001
    2广西医科大学第一附属医院神经外科，广西壮族自治区南宁市530021
  • 收稿日期:2011-04-14 修回日期:2011-06-22 出版日期:2011-12-03 发布日期:2011-12-03
  • 作者简介:唐景峰★，男，1975年生，广西壮族自治区灌阳县人，瑶族，2007年广西医科大学毕业，硕士，副主任医师，主要从事脑血管疾病防治和颅底显微外科研究。 tangjingfeng152@163.com

Expression of cancer-testis antigen in glioma stem cells

Tang Jing-Feng1, Xiao Shao-wen2, Huang Yong-wang1, Yang Bao-hua1, Du Yi-qing1   

  1. 1Department of Neurosurgery, Affiliated Hospital of Guilin Medical College, Guilin  541001, Guangxi Zhuang Autonomous Region, China
    2Department of Neurosurgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-14 Revised:2011-06-22 Online:2011-12-03 Published:2011-12-03
  • About author:Tang Jing-feng★, Master, Associate chief physician, Department of Neurosurgery, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China tangjingfeng152@163.com

PDF

332

摘要：

背景：癌-睾丸抗原基因在胶质瘤干细胞表达还不甚清楚。
目的：检测癌-睾丸抗原基因在神经胶质瘤干细胞中的表达。
方法：通过神经球培养的方法而分离干细胞。以半定量PCR及实时荧光定量PCR检测癌-睾丸抗原基因在母细胞、干细胞与分化细胞中的表达。
结果与结论：以神经球培养方式从胶质瘤细胞系中可分离出肿瘤球，将肿瘤球培养在含血清的培养基中，它的形态呈贴壁分化，且与母细胞没有区别。半定量PCR及实时荧光定量PCR检测发现与母细胞和分化细胞相比，癌-睾丸抗原基因在干细胞中的表达最高。提示癌-睾丸抗原基因可能为肿瘤干细胞的表面抗原。

关键词: 癌-睾丸抗原, 肿瘤干细胞, 胶质瘤, 母细胞, 干细胞

Abstract:

BACKGROUND: It is still unclear on the expression of cancer-testis antigen genes in glioma stem cells.
OBJECTIVE: To detect the expression of cancer testis antigen genes in glioma stem cells.
METHODS: Stem cells were cultured and isolated by neurosphere culture methods in DMEM/F12. Then the expression of cancer testis antigen genes were analyzed among parent cells, stem cells and differentiated cells by using semiquantitative RT-PCR and real time PCR.
RESULTS AND CONCLUSION: We isolated tumor spheres from glioma cell lines by neurosphere culture. And then tumor spheres were cultured in medium containing serum, the morphology of the tumor spheres changed to adherent and they were not distinguishable from the parent cells. The expression of cancer testis antigen genes were analyzed by semiquantitative RT-PCR and quantitative RT-PCR and it was found that the expression were highest in stem cells compared with parent cells and differentiated cells. Cancer testis antigen genes may present as surface antigens in cancer stem cells and these findings suggest that cancer testis antigen genes may be helpful for targeted vaccine therapy against cancer stem cells in glioma patients.

中图分类号: