中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (41): 7730-7733.doi: 10.3969/j.issn.1673-8225.2011.41.032

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

阿魏酸及其酯化产物与体外培养大鼠大脑皮质神经元的存活

盛艳梅1，张  静2，罗维早3，张  艺2   

  1. 1成都医学院药学院，四川省成都市 610083
    2成都中医药大学民族医药研究院，四川省成都市 610075
    3重庆市中药研究院，重庆市 400065
  • 收稿日期:2011-03-14 修回日期:2011-04-19 出版日期:2011-10-08 发布日期:2011-10-08
  • 通讯作者: 张艺，博士，研究员，成都中医药大学民族医药研究院，四川省成都市610075 9006zmy@cdutcm.edu.cn
  • 作者简介:盛艳梅☆，女，1979年生，湖南省益阳市人，汉族，2010年成都中医药大学毕业，博士，讲师，主要从事中药与民族药药效及毒理研究。 shengyanmei@163.com
  • 基金资助:

    国家自然科学基金资助项目(30672602)《基于钙信号通路的脑靶向神经保护中药有效组分的筛选方法研究》。

Effect of ferulic acid and its esterified product on survival of rat cerebral cortex neurons cultured in vitro

Sheng Yan-mei1, Zhang Jing2, Luo Wei-zao3, Zhang Yi2   

  1. 1College of Pharmacy, Chengdu Medical College, Chengdu  610083, Sichuan Province, China
    2National Medical Research Institute, Chengdu University of Traditional Chinese Medicine, Chengdu  610075, Sichuan Province, China
    3 Chongqing Academy of Chinese Materia Medica, Chongqing  400065, China
  • Received:2011-03-14 Revised:2011-04-19 Online:2011-10-08 Published:2011-10-08
  • Contact: Zhang Yi, Doctor, Professor, National Medical Research Institute, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China 9006zmy@cdutcm.edu.cn
  • About author:Sheng Yan-mei☆, Doctor, Lecturer, College of Pharmacy, Chengdu Medical College, Chengdu 610083, Sichuan Province, China shengyanmei@163.com
  • Supported by:

    the National Natural Science Foundation of China, No.30672602*

PDF

490

被引次数

8

摘要：

背景：大量体外筛选中得到的中药脑神经保护活性成分，多因脂溶性低难以透过血脑屏障而限制了其临床应用。
目的：比较阿魏酸及其酯化产物阿魏酸甲酯、乙酯对原代培养的大鼠大脑皮质神经元体外存活的保护作用。
方法：出生1 d内的乳鼠采用酶消化法分离培养大脑皮质神经元。培养6 d后，分别进行阿魏酸、阿魏酸甲酯及乙酯干预，继续培养1 d。
结果与结论：形态学观察结果显示细胞生长良好，NSE染色检查表明培养6 d的存活细胞大部分均为神经元。MTT比色法测量结果显示，与空白对照组比较，阿魏酸仅在质量浓度为200 mg/L时有明显促进大脑皮质神经元存活的作用，而阿魏酸甲酯、乙酯在质量浓度为0.16~20 mg/L时均能明显促进神经元体外存活，且作用趋势、作用强度相近。表明阿魏酸的酯化产物阿魏酸甲酯和阿魏酸乙酯均能促进原代培养的大脑皮质神经元体外存活，显示出较好的脑神经元保护作用，且活性比阿魏酸更强。

关键词: 川芎, 阿魏酸, 酯化产物, 大脑皮质神经元, 细胞培养, 脑缺血

Abstract:

BACKGROUND: Many active components of traditional Chinese medicine of brain nerve protection screened in vitro have been limited in clinical application because of difficulties in passing through blood brain barrier due to low liposolubility.
OBJECTIVE: To compare the protective effect of ferulic acid and its esterified products ferulic acid methyl ester and ethyl ester on in vitro survival of primary cultred cerebral cortex neurons. 
METHODS: Cerebral cortex neurons from rats at postnatal 1 day were isolated by enzyme digestion method. After 6 days of culture, these neurons were interfered with ferulic acid, ferulic acid methyl ester and ethyl ester and then cultured for 1 day.
RESULTS AND CONCLUSION: Morphological results showed that cells well grew. Neuron specific enolase staining showed that after 6 days of culture, most of surviving cells were neurons. MTT examination results showed that compared with blank control group, 200 mg/L ferulic acid could obviously promote the survival of cerebral cortex neurons, while 0.16-20 mg/L ferulic acid methyl ester and ethyl ester could obviously promote the in vitro survival of neurons, with similar tendency and strength. These results suggest that ferulic acid esterified products ferulic acid methyl ester and ethyl ester can promote the in vitro survival of primary cultured cerebral cortex neurons, exhibiting good protective effects on brain neurons, with stronger activity than ferulic acid.

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