中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (32): 5983-5986.doi: 10.3969/j.issn.1673-8225.2011.32.021

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

人脐带间充质干细胞体外分泌的伤口愈合相关因子

张美荣，孙亚如，王  丽，张学峰，胡建霞，周  倩，高  宏，王颜刚   

  1. 青岛大学医学院附属医院干细胞研究中心，山东省青岛市  266003
  • 收稿日期:2011-02-14 修回日期:2011-04-11 出版日期:2011-08-06 发布日期:2011-08-06
  • 通讯作者: 孙亚如，硕士，青岛大学医学院附属医院干细胞研究中心，山东省青岛市 266003 thomassun2008@yahoo.com.cn
  • 作者简介:张美荣★，女，1978年生，吉林省吉林市人，汉族，2006年中国协和医科大学毕业，硕士，主要从事干细胞技术方面的研究。 zhangmeirong0431@yahoo.com.cn

Wound healing related trophic factors derived from human umbilical cord mesenchymal stem cells

Zhang Mei-rong, Sun Ya-ru, Wang Li, Zhang Xue-feng, Hu Jian-xia, Zhou Qian, Gao Hong, Wang Yan-gang   

  1. Stem Cell Research Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao  266003, Shandong Province, China
  • Received:2011-02-14 Revised:2011-04-11 Online:2011-08-06 Published:2011-08-06
  • Contact: Sun Ya-ru, Master, Stem Cell Research Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China thomassun2008@yahoo.com.cn
  • About author:Zhang Mei-rong★, Master, Stem Cell Research Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, Shandong Province, China zhangmeirong0431@yahoo.com.cn

PDF

480

被引次数

4

摘要：

背景：目前对人脐带间充质干细胞培养上清液中伤口愈合相关因子定量检测研究的报道较少。
目的：检测人脐带间充质干细胞培养上清中伤口愈合相关因子白细胞介素6，白细胞介素8，转化生长因子1，单核细胞趋化蛋白1，血管内皮生长因子，GM-CSF和TIMP-1的分泌水平。
方法：以胎牛血清替代物CCS1107为培养基添加物培养人脐带间充质干细胞。将培养获得的P2代细胞以1.1×104/cm2的接种密度接种于直径100 mm无菌培养皿中，培养96 h后，收集上清液并对其中所含伤口愈合相关因子进行ELISA定量检测。
结果与结论：10份不同个体间的因子分泌量差异无显著性意义，男女间表达量差异无显著性意义。高表达白细胞介素6, 白细胞介素8，单核细胞趋化蛋白1和组织基质金属蛋白酶抑制剂1，其中单核细胞趋化蛋白1和组织基质金属蛋白酶抑制剂1的表达量最高。结果证实，人脐带间充质干细胞培养上清液中含有大量伤口愈合相关因子。

关键词: 人脐带间充质干细胞, 伤口愈合, 白细胞介素, 单核细胞趋化蛋白1, 组织基质金属蛋白酶抑制剂1

Abstract:

BACKGROUND: Few reports have quantitatively determined the levels of wound healing related factors in the conditioned media of human umbilical cord mesenchymal stem cells (HUMSCs).
OBJECTIVE: To measure the secretion levels of interleukin-6 (IL-6), IL-8, transforming growth factor 1 (TGF-1), monocyte chemoattractant protein 1 (MCP-1), vascular endothelial growth factor (VEGF), GM-CSF and TIMP-1 in HUMSCs derived conditioned media of 10 different individuals.
METHODS: For culturing HUMSCs, CCS1107 was used as fetal bovine serum supplement to the culture medium. 1.1x104/cm2 P2 cells were seeded in 100 mm diameter dishes, and after 96 hours’ culture, the supernatant was collected and applied to ELISA measurement.
RESULTS AND CONCLUSION: No significant secretion difference among 10 different individuals and between genders was observed. Of total factors being measured, the levels of MCP-1 and TIMP-1 were the highest. These results indicated that HUMSCs derived conditioned media contains numerous kinds of wound healing related factors, which may clinically provide a novel therapeutic method for treating various wound healing related impairments.

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