中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (2): 213-218.doi: 10.3969/j.issn.1673-8225.2011.02.006

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

建立桡骨缺损模型：骨缺损标准尺寸的确立

赵明东1，潘志宏1，朱梁豫1，尹望平1，董  健2   

  1. 1复旦大学附属金山医院骨科，上海市  200540
    2复旦大学附属中山医院骨科，上海市  200032
  • 收稿日期:2010-09-02 修回日期:2010-12-07 出版日期:2011-01-08 发布日期:2011-01-08
  • 通讯作者: 董健，教授，博士生导师，主任医师，复旦大学附属中山医院骨科，上海市 200032 doctor_dong@ 126.com
  • 作者简介:赵明东★，男，1978年生，山东省莱阳市人，硕士， 2008年复旦大学上海医学院毕业，主要从事骨创伤及组织工程研究。 zhaomingdong@medmail.com.cn
  • 基金资助:

    上海市金山区科委基金项目(2009-3-01)。

Construction of radius defect models: To standardize critical size defects

Zhao Ming-dong1, Pan Zhi-hong1, Zhu Liang-yu1, Yin Wang-ping1, Dong Jian2   

  1. 1Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai  200540, China
    2Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai  200032, China
  • Received:2010-09-02 Revised:2010-12-07 Online:2011-01-08 Published:2011-01-08
  • Contact: Dong Jian, Professor, Doctoral supervisor, Chief physician, Department of Orthopaedics, Zhongshan Hospital, Fudan University, Shanghai 200032, China doctor_dong@126. com
  • About author:Zhao Ming-dong★, Master, Department of Orthopaedics, Jinshan Hospital, Fudan University, Shanghai 200540, China zhaomingdong@ medmail.com.cn
  • Supported by:

    the Foundation of Science Commission of Jinshan District of Shanghai, No. 2009-3-01

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摘要：

背景：不同研究所采用的桡骨骨缺损模型长度均不同，目前尚没有统一的桡骨缺损标准尺寸。
目的：对兔桡骨缺损模型施行标准化研究再探讨，以期获得相对更可靠的兔桡骨缺损标准尺寸。
方法：选用健康6月龄新西兰大白兔40只，双侧桡骨共80侧，在麻醉下行兔桡骨手术造成桡骨完全骨膜缺损，将桡骨随机分为5组，分别为缺损1.0，1.2，1.4，1.7和2.0 cm组，每组16侧。分别于术后12周行X射线、大体解剖、CT三维重建和组织切片等检查，观察各组骨缺损愈合情况。
结果与结论：建模后12周可见缺损1.0 cm组至缺损2.0 cm组愈合率逐步下降，1.4 cm骨缺损组愈合率低于1.0及1.2 cm组(P < 0.05)。1.4，1.7，2.0 cm骨缺损组X射线评分及CT评分值均低于1.0及1.2 cm骨缺损组(P < 0.05)，说明骨缺损尺寸在1.2 cm以下时，桡骨自行愈合率相对较高。结果提示实验成功建立了兔桡骨骨缺损模型，桡骨中段缺损(包括骨膜)尺寸≥1.4 cm更加可靠。

关键词: 骨缺损, 兔, 动物模型, 桡骨, 骨组织工程

Abstract:

BACKGROUND: The length of radial bone defects are variant in different studies, which lack of reliable critical size defects (CSD).
OBJECTIVE: To investigate the standardization of the model of radial bone defect in rabbits, so as to obtain relatively reliable CSD.
METHODS: A total of 40 6-month New Zealand white rabbits including 80 radial bones were prepared for bone defect modelsAccording to the principle of randomization, the eighty radii which were operated for different length in bone defects were divided into 1.0-, 1.2-, 1.4-, 1.7- and 2.0-cm groups. X-ray film, gross anatomy, CT three-dimensional reconstruction, as well as tissue slices of the radius bone defect were carried out at 12 weeks after operation to evaluate the bone defect healing.
RESULTS AND CONCLUSION: At 12 weeks after operation, the healing rate was decreased from the 1.0- to 2.0-cm group, and the 1.4-cm group was lower than that of the 1.0- and 1.2-cm groups (P < 0.05). X-ray and CT grade of healing up of the 1.4-, 1.7-, 2.0-cm groups were smaller than those of the 1.0- and 1.2-cm groups (P < 0.05), which demonstrated that the bone healed well within 1.2-cm defects. The rabbit radial bone defect models were constructed successfully, which can be obtained by greater than or equal to 1.4-cm middle segmental defects (involving periosteum).

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