中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (10): 1893-1896.doi: 10.3969/j.issn.1673-8225.2011.10.041

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

骨髓液促进鸡胚绒毛尿囊膜血管的生成

唐  昱1，钟志英2，盛国太1，葛郁之1   

  1. 1江西省人民医院心血管内科，江西省心血管病研究所，江西省南昌市  330006
    2南昌大学第四附属医院心内科，江西省南昌市  330000
  • 收稿日期:2010-10-13 修回日期:2011-01-04 出版日期:2011-03-05 发布日期:2011-03-05
  • 通讯作者: 唐昱，江西省人民医院心血管内科，江西省南昌市 330006
  • 作者简介:唐昱★，男，1977年，江西省临川市人，汉族，2006年毕业于南昌大学医学院，硕士，主治医师。
  • 基金资助:

    江西省卫生厅中医药研究基金(2009A077)。

Promoting effect of bone marrow serum on angiogenesis of chick embryo chorioallantoic membrane

Tang Yu1, Zhong Zhi-ying2, Sheng Guo-tai1, Ge Yu-zhi1     

  1. 1Department of Cardiovascular Medicine, Jiangxi Provincial People’s Hospital, Jiangxi Institute of Cardiovascular Disease, Nanchang 330006, Jiangxi Province, China
    2Department of Cardiology, Fourth Affiliated Hospital of Nanchang University, Nanchang  330000, Jiangxi Province, China
  • Received:2010-10-13 Revised:2011-01-04 Online:2011-03-05 Published:2011-03-05
  • Contact: Tang Yu, Master, Attending physician, Department of Cardiovascular Medicine, Jiangxi Provincial People’s Hospital, Jiangxi Institute of Cardiovascular Disease, Nanchang 330006, Jiangxi Province, China yutangya@yahoo.com.cn
  • About author:Tang Yu★, Master, Attending physician, Department of Cardiovascular Medicine, Jiangxi Provincial People’s Hospital, Jiangxi Institute of Cardiovascular Disease, Nanchang 330006, Jiangxi Province, China yutangya@yahoo.com.cn
  • Supported by:

    the Grant for Traditional Chinese Medicine Research of Health Department of Jiangxi Province, No. 2009A077*

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摘要：

背景：生长因子能促进侧支血管的发育，且多种因子协同效果更为明显，骨髓液中富含多种生长因子。
目的：观察血管内膜损伤后的骨髓液对鸡胚绒毛尿囊膜血管生成的作用。
方法：受精蛋70枚在(37.5±0.5) ℃条件下孵育，第7天开窗，第8天将存活鸡胚随机分为生理盐水组、正常血清组、正常骨髓液组、损伤血清组、损伤骨髓液组以及血管内皮生成因子组，每组10枚，分别滴加5 μL兔正常血清、5 μL兔骨髓液、5 μL兔血管内膜损害血清、5 μL兔血管内膜损害骨髓液、5 μL生理盐水及0.3 μg 血管内皮生长因子进鸡胚绒毛尿囊膜中，连续3 d。数码相机拍照后平铺于载玻片上，计数鸡胚绒毛尿囊膜新生的血管数目。
结果与结论：与正常血清组相比，正常骨髓液组、血管内膜损害血清组鸡胚绒毛尿囊膜新生的血管总数明显增多，大中血管明显增生；且血管内膜损害血清组大、中血管数更为明显增加。提示正常骨髓液具有明显的促进鸡胚绒毛尿囊膜模型血管生成的作用，其强度优于血管内皮生长因子；血管内膜损伤第7天的血清和骨髓液能够明显的促进鸡胚绒毛尿囊膜上的血管生成，其强度优于血管内皮生长因子组。

关键词: 鸡胚, 尿囊膜, 血管生成, 骨髓, 内膜损伤

Abstract:

BACKGROUND: Growth factor can promote the development of collateral vessels. The synergistic effects of multiple factors are obvious. Many kinds of growth factors are enriched in bone marrow serum.
OBJECTIVE: To determine effects of bone marrow serum on angiogenesis in chick chorioallantoic membranes following vascular intimal injury.
METHODS: A total of 70 fertilized chick eggs were incubated at (37.5±0.5) ℃ and made a window in every egg at day 7. The survival chick embryos were randomly divided into six groups: normal saline group, normal blood serum group, normal bone marrow serum group, blood serum of rabbit suffered with vascular intimal injury group, bone marrow serum of rabbit suffered with vascular intimal injury group and vascular endothelial growth factor group (n=10), which were respectively treated with 5 μL rabbit normal blood serum, 5 μL rabbit normal bone marrow serum, 5 μL blood serum of rabbit suffered with vascular intimal injury, 5 μL bone marrow serum of rabbit suffered with vascular intimal injury, 5 μL normal saline and 0.3 μg vascular endothelial growth factor in chick chorioallantoic membranes, for 3 consecutive days. Pictures were taken by a digital camera, and the total number of the vessels around the disc was measured.
RESULTS AND CONCLUSION: The total numbers of vessels in normal bone marrow serum group and blood serum of rabbit suffered with vascular intimal injury group were obviously higher than that of normal blood serum group. Large and middle vessels were obviously proliferated. Large and middle vessels were more observed in the blood serum of rabbit suffered with vascular intimal injury group. These indicated that normal bone marrow serum can stimulate angiogenesis in models of chick chorioallantoic membranes. Compared with vascular endothelial growth factor, both the normal bone serum and the bone marrow serum of rabbit suffered from vascular intimal injury were more effective in angiogenesis. The blood serum and bone marrow serum from vascular intimal injured rabbit at day 7 can significantly promote angiogenesis in chick chorioallantoic membranes, which was better than the vascular endothelial growth factor group.

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