中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (1): 68-73.doi: 10.3969/j.issn.1673-8225.2011. 01.015

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

基质细胞衍生因子1α及受体CXCR4对颈总动脉球囊损伤血管平滑肌细胞和骨髓基质细胞的影响

蔡文玮1，方宁远2，盛  净1，马绍骏1，程智慧1   

  1. 1上海交通大学医学院附属第九人民医院老年病科，上海市 200011
    2上海交通大学附属仁济医院老年病科，上海市  200127
  • 收稿日期:2010-07-23 修回日期:2010-09-20 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 盛净，硕士，主任医师，上海交通大学医学院附属第九人民医院老年病科，上海市 200011 shj-60@hotmail.com
  • 作者简介:蔡文玮☆，女，1967年生，福建省福州市人，汉族，2009年上海第二医科大学毕业，博士，副主任医师，主要从事老年医学方面的研究。 alonhuc@sohu.com
  • 基金资助:

    上海市卫生局基金资助项目(2008088)。

Influence of stromal cell-derived factor-1 alpha and its receptor CXCR4 on vascular smooth muscle cells and bone marrow stromal cells in common carotid artery balloon injury models  

Cai Wen-wei1, Fang Ning-yuan2, Sheng Jing1, Ma Shao-jun1, Cheng Zhi-hui1   

  1. 1Department of Geriatrics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
    2Department of Geriatrics, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200127, China
  • Received:2010-07-23 Revised:2010-09-20 Online:2011-01-01 Published:2011-01-01
  • Contact: Sheng Jing, Master, Chief physician, Department of Geriatrics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China shj-60@hotmail.com
  • About author:Cai Wen-wei☆, Doctor, Associate chief physician, Department of Geriatrics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China alonhuc@sohu.com
  • Supported by:

    the Grant of Health Bureau of Shanghai City, No. 2008088

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摘要：

背景：在细胞因子的作用下，骨髓基质细胞可以向内皮祖细胞分化，并迁移至损伤部位增殖，参与血管新生内膜的修复。
目的：观察基质细胞衍生因子1α及其受体CXCR4在大鼠颈总动脉球囊损伤后对血管平滑肌细胞和骨髓基质细胞的影响。
方法：体外培养大鼠颈总动脉球囊损伤后各时间点(即刻、1，4，7，14及1个月)的血管平滑肌细胞和骨髓基质细胞，检测损伤后平滑肌细胞中基质细胞衍生因子1α及骨髓基质细胞中CXCR4的表达情况，并应用基质细胞衍生因子1α siRNA转染损伤后血管平滑肌细胞，同时应用transwell 小室观察基质细胞衍生因子1α和损伤后血管平滑肌细胞对骨髓基质细胞的趋化作用。
结果与结论：大鼠颈总动脉球囊损伤后，平滑肌细胞中基质细胞衍生因子1α mRNA表达开始增加，至损伤第7天后逐渐减弱；血管损伤后4 d起，骨髓基质细胞中CXCR4 mRNA开始出现，并持续表达；transwell小室显示损伤后的骨髓基质细胞对基质细胞衍生因子1α的趋化性增强；CXCR4受体拮抗剂AMD3100可以明显减弱基质细胞衍生因子1α对骨髓基质细胞的趋化作用；损伤后的血管平滑肌细胞对骨髓基质细胞的趋化作用强于基质细胞衍生因子1α(P< 0.05)，应用AMD3100干预或细胞内转染基质细胞衍生因子1α siRNA后，此趋化作用亦减弱(P < 0.05)。结果提示，基质细胞衍生因子1α/CXCR4轴在骨髓基质细胞向损伤血管迁移中起重要的作用。

关键词: 基质细胞衍生因子1&alpha, 骨髓基质细胞, 血管平滑肌细胞, 颈总动脉, 内膜增生

Abstract:

BACKGROUND: Bone marrow stromal cells (BMSCs) can differentiate into endothelial progenitor cells by the action of cytokine and migrate to the injured site to participate in vascular intima repair.
OBJECTIVE: To observe the influence of the interaction between stromal cell-derived factor-1α (SDF-1α) and its receptor CXCR4 on vascular smooth muscle cells and BMSCs in the rat common carotid artery balloon injury model.
METHODS: Vascular smooth muscle cells and BMSCs were cultured in vitro according to different time points just after surgery (0), 1, 4, 7, 14 days and 1 month after balloon injury in rat common carotid artery. SDF-1α mRNA expressions were detected in vascular smooth muscle cells while CXCR4 mRNA expressions were detected in BMSCs. SDF-1α siRNA was transfected into vascular smooth muscle cells and transwell body was used to measure the chemotaxis of SDF-1α or vascular smooth muscle cells to BMSCs.
RESULTS AND CONCLUSION: SDF-1α mRNA expression began to increase just after balloon injury, and then subsided gradually on day 7. CXCR4 mRNA began to express on day 4 after balloon injury and lasted. Transwell cabin demonstrated that SDF-1 chemotaxis increased in injured BMSCs than in normal ones; the antagonist of CXCR4 receptor AMD3100 could weaken SDF-1 chemotaxis significantly. The chemotaxis of injured vascular smooth muscle cells to BMSCs was stronger than SDF-1 (P < 0.05) and the action could be weakened by AMD3100 or transfection of SDF-1α siRNA in vascular smooth muscle cells (P < 0.05). Results indicated that SDF-1α/CXCR4 should be important in the process of BMSCs migration to injured vessels.

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