中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (1): 159-162.doi: 10.3969/j.issn.1673-8225.2011. 01.036

• 干细胞临床实践 clinical practice of stem cells • 上一篇    下一篇

嗅鞘细胞联合神经干细胞移植治疗小儿脑发育不全

陶晓宇1，刘  波1，滕晓华1，邓士杰2，谢庙均3，徐  蓉1，卢  明1   

  1. 湖南师范大学第二附属医院，解放军第163医院；1神经外科，2医学影像中心，湖南省长沙市 410003；3武装警察部队成都指挥学院教研部，四川省成都市610213
  • 收稿日期:2010-08-04 修回日期:2010-10-18 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 卢明，博士，主任医师，湖南师范大学第二附属医院，解放军第163医院神经外科，湖南省长沙市 410003
  • 作者简介:陶晓宇★，男，1983年生，湖南省安化县人，汉族，湖南师范大学在读硕士，主要从事神经修复方面的研究。 lichenyu0913@yahoo.com.cn
  • 基金资助:

    湖南省科技厅项目(2008FJ3061)；全军“十一五”科技攻关项目(06G044)。

Combined transplantation of neural stem cells and olfactory ensheathing cells for the treatment of cerebral hypoplasia

Tao Xiao-yu1, Liu Bo1, Teng Xiao-hua1, Deng Shi-jie2, Xie Miao-jun3, Xu Rong1, Lu Ming1   

  1. 1Second Affiliated Hospital, Hunan Normal University, Department of Neurosurgery, the 163 Hospital of Chinese PLA, Changsha  410003, Hunan Province, China
    2Second Affiliated Hospital, Hunan Normal University, Medical Image Center, the 163 Hospital of Chinese PLA, Changsha  410003, Hunan Province, China
    3Department of Teaching and Research, Chinese People's Armed Police Force Academy in Chengdu, Chengdu 610213, Sichuan Province, China
  • Received:2010-08-04 Revised:2010-10-18 Online:2011-01-01 Published:2011-01-01
  • Contact: Lu Ming, Doctor, Chief physician, Second Affiliated Hospital, Hunan Normal University, Department of Neurosurgery, the 163 Hospital of Chinese PLA, Changsha 410003, Hunan Province, China
  • About author:Tao Xiao-yu★, Studying for master’s degree, Second Affiliated Hospital, Hunan Normal University, Department of Neurosurgery, the 163 Hospital of Chinese PLA, Changsha 410003, Hunan Province, China lichenyu0913@yahoo.com.cn
  • Supported by:

    the Grant of Department of Science and Technology of Hunan Province, No. 2008FJ3061; the Key Science and Technology Program of the Chinese PLA during the 11th “Five-Year” Plan Period, No. 06G044

PDF

572

被引次数

3

摘要：

背景：近年来，干细胞替代治疗为神经再生提供了新的方法，但干细胞移植治疗小儿脑发育不全的相关报道甚少。
目的：验证嗅鞘细胞联合神经干细胞移植治疗小儿脑发育不全的有效性。
方法：2009-10解放军第163医院收治了1例脑发育不全的患者，给予物理降温、神经营养、维持水电解质平衡、高压氧治疗的同时，以L3~4腰椎间隙为穿刺点，将配置好的神经干细胞约8×107个和嗅鞘细胞约4×107个缓慢注入蛛网膜下腔，每2周1次，2次为 1 个疗程，共治疗1个疗程。从体温恢复程度、双手精细动作、运动协调性、语言等方面来评价移植治疗效果。
结果与结论：与移植前比较，嗅鞘细胞联合神经干细胞移植1个月后患儿体温恢复正常，四肢肌力明显下降，2个月后可以独自端坐，3个月后可以扶梯行走，有简单的语言表达。提示嗅鞘细胞联合神经干细胞移植治疗小儿脑发育不全是有效的，能恢复其中枢神经功能。

关键词: 脑发育不全, 神经干细胞, 嗅鞘细胞, 细胞移植, 干细胞移植

Abstract:

BACKGROUND: Recent years, stem cell replacement therapy furnishes a new method for neural regeneration. But until now, we have not found the reports about stem cell transplantation for treating cerebral hypoplasia.
OBJECTIVE: To explore the effectiveness of combined transplantation of neural stem cells and olfactory ensheathing cells for the repair of cerebral hypoplasia.
METHODS: In October, 2009, a cerebral hypoplasia patient was enrolled at the 163 Hospital of the Chinese People’s Liberation Army. We used the treatment of physical cooling, neurotrophic, maintenance of water-electrolyte balance, hyperbaric oxygen. We selected the point of puncture between the L3-4 of the spine and injected neural stem cells (about 8×107) and olfactory ensheathing cells (about 4×107) slowly into the subarachnoid space, once two weeks, twice as a course, totally one course. We assessed the results of transplantation treatment by drop of body temperature, fine motor, motor coordination and language expression.
RESULTS AND CONCLUSION: The body temperature decreased to normal level, and muscular tension reduced obviously after one month, he could sit up by himself after two months, walk under the help of handrail, and have simple language expression after three months. These indicated that it is effective to treat cerebral hypoplasia and can recover central nerve function by combined transplantation of neural stem cells and olfactory ensheathing cells.

中图分类号: