中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (51): 9577-9581.doi: 10.3969/j.issn.1673-8225.2010.51.017

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

负载可降解缓释微球壳聚糖支架的生物相容性

熊敏剑1，李晓峰1，黄山虎1，徐小丽2，谢  宇3，洪小伟3   

  1. 1南昌大学第一附属医院骨科，江西省南昌市 330006；2华中科技大学同济医学院附属协和医院血液科，湖北省武汉市 430022；3南昌航空大学环境与化学工程学院材料化学系，江西省南昌市330063
  • 出版日期:2010-12-17 发布日期:2010-12-17
  • 通讯作者: 李晓峰，博士，副主任医师，南昌大学第一附属医院骨科，江西省南昌市 330006 doctorli000@163.com
  • 作者简介:熊敏剑★，男，1982年生，江西省丰城市人，汉族，南昌大学第一附属医院在读硕士，主要从事关节软骨修复方面的研究。fcxiong@yahoo.com.cn
  • 基金资助:

    江西省科技厅支撑计划(2008)，课题名称：缓释TGF-β1的壳聚糖支架修复关节软骨缺损的实验研究。

Biocompatibility of sustained-releasing microspheres loading chitosan scaffolds

Xiong Min-jian1, Li Xiao-feng1, Huang Shan-hu1, Xu Xiao-li2, Xie Yu3, Hong Xiao-wei3   

  1. 1 Department of Orthopaedics, The First Affiliated Hospital of Nanchang University, Nanchang   330006, Jiangxi Province, China; 2 Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan   430022, Hubei Province, China; 3 Department of Materials Chemistry, Environmental and Chemical Engineering College of Nanchang Hangkong University, Nanchang   330063, Jiangxi Province, China
  • Online:2010-12-17 Published:2010-12-17
  • Contact: Li Xiao-feng, Doctor, Associate chief physician, Department of Orthopaedics, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China doctorli000@163.com
  • About author:Xiong Min-jian★, Studying for master’s degree, Department of Orthopaedics, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China fcxiong@yahoo.com.cn
  • Supported by:

    Pillar Program of Jiangxi Provincial Science and Technology Bureau, No. 2008*

PDF

446

被引次数

6

摘要：

背景：负载缓释转化生长因子β1微球壳聚糖支架在体外能够促进软骨细胞生长，且可以诱导骨髓间充质干细胞向软细胞分化，有望作为软骨缺损修复的组织工程材料，然而要进行相应体内实验，其生物相容性是不容忽视的。
目的：制备负载缓释微球壳聚糖支架并对其生物相容性进行体内外评价。
方法：以溶血试验、急性毒性实验、皮内刺激实验、热源性实验、肌内植入实验，评价自制负载缓释微球壳聚糖支架的生物相容性。
结果与结论：支架溶血率为1.6%，镜下未见明显红细胞破坏；材料急性毒性评价程度为无毒；皮内原发刺激记分及原发刺激指数均为0；热源性实验体温升高为(0.17±0.06) ℃；肌内植入实验大鼠均成活，全身良好、无感染，4周左右新生毛正常分布，8周大体观察支架周围血管明显增多，与周围肌组织整合良好，心肝肺肾等内脏均无特殊变化，随时间延长，淋巴细胞浸润逐渐减少，可见血管及纤维长入支架，包裹逐渐变薄，支架渐降解。结果说明负载微球多孔壳聚糖支架具有优良的生物相容性。

关键词: 缓释微球, 壳聚糖, 支架, 生物相容性, 生物材料

Abstract:

BACKGROUND: Chitosan scaffolds loading sustained-releasing transforming growth factor β microspheres can promote chondrocyte grow in vitro, and induce bone marrow mesenchymal stem cells differentiation into chondrocytes. Chitosan scaffolds loading sustained-releasing microspheres are expected to be a booming tissue engineered material for the treatment of cartilage defects. However, the biocompatibility is not disregardful for in vivo experiments.
OBJECTIVE: To prepare chitosan scaffolds loading sustained-releasing microspheres, and evaluate its biocompatibility in vivo and in vitro.
METHODS: By using hemolytic test, acute systemic toxicity test, intracutaneous stimulation test, heat source experiment and intramuscular implantation test, the biocompatibility of the self-made chitosan scaffolds loading sustained-releasing microspheres was comprehensively evaluated.
RESULTS AND CONCLUSION: The hemolytic rate of chitosan scaffolds was 1.6%, there was no significant destruction of red blood cells observed via the microscope; the acute toxicity was evaluated as non-toxicl; the intracutaneous primary stimulation scores and the primary stimulation index were both 0; in the heat source experiment, the body temperature raise to (0.17±   0.06) ℃; rats underwent intramuscular implantation all survived well without systemic infection, and newborn hair normally distributed at 4 weeks; Naked-eye observed that there were significantly increased peripheral vascular stents at 8 weeks, which well integrated with the surrounding muscle tissue, while other internal organs such as heart , liver, lung and kidney were ordinary. Along with time prolonged, infiltration of lymphocytes was gradually decreased, blood vessels and fibrous tissues were visible to grow into the scaffolds, wrapped fibrous tissues were gradually thinner, chitosan scaffolds were gradually degraded. Chitosan scaffold loading porous microspheres has excellent biocompatibility.

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