中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (45): 8487-8490.doi: 10.3969/j.issn.1673-8225.2010.45.030

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

诱导性多能干细胞的重编程

卞合涛1，黄  卫1，李士勇2   

  1. 南昌大学第二附属医院，1神经内科，2分子中心实验室，江西省南昌市  330006
  • 出版日期:2010-11-05 发布日期:2010-11-05
  • 通讯作者: 李士勇，博士，南昌大学第二附属医院分子中心实验室，江西省南昌市 330006 syliwy@gmail.com
  • 作者简介:卞合涛★，男，1982年生，山东省临沂市人，南昌大学第二附属医院神经内科在读硕士，主要从事干细胞的相关研究。 hetaobian@163.com

Reprogramming of induced pluripotent stem cells

Bian He-tao1, Huang Wei1, Li Shi-yong2   

  1. 1 Department of Neurology, 2 Molecule Center Laboratory, Second Affiliated Hospital, Nanchang University, Nanchang  330006, Jiangxi Province, China 　
  • Online:2010-11-05 Published:2010-11-05
  • Contact: Li Shi-yong, Doctor, Molecule Center Laboratory, Second Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China syliwy@gmail.com
  • About author:Bian He-tao★, Studying for master’s degree, Department of Neurology, Second Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China hetaobian@163.com

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摘要：

背景：诱导性多能干细胞在形态学、表观遗传修饰、基因表达等方面与胚胎干细胞十分相似，但对其研究尚在初级阶段，且安全性和高效性是诱导性多能干细胞应用面临的主要问题。
目的：对诱导性多能干细胞的实验研究进行综述。
方法：应用计算机检索Medline数据库、Ovid数据库、CNKI、EBSCO数据库与诱导性多能干细胞相关文献。检索词为“诱导性多能干细胞、胚胎干细胞、体细胞、重编程、诱导、转录因子，induced pluripotent stem cells、Embryonic stem cells、Somatic cells、reprogramming、induction、Transcription factor”。
结果与结论：诱导性多能干细胞获得主要通过导入特定的基因，使体细胞重编程，其特定基因包括Oct 4、Sox2、c-Myc、Klf4、Nanog、Lin-28。目前认为，体细胞通过导入外源性的转录因子可以激活内源性的Oct4 与Sox2 的表达，并在细胞内迅速形成一个自我调节的网络用以维持诱导性多能干细胞的多能性，并使其保持多能性。但对于重编程的具体机制仍不是很清楚。

关键词: 诱导性多能干细胞, 胚胎干细胞, 重编程, 转录因子, 干细胞

Abstract:

BACKGROUND: Although induced pluripotent stem cells (iPSCs) have similar morphological, epigenetic and gene expression characteristics as embryonic stem cells (ESCs), this research is at a very early stage and many fundamental questions remain such as security and efficiency.
OBJECTIVE: To review empirical study of iPSCs.
METHODS: A computer-based online search of Medline, Ovid, CNKI, and EBSCO databases was undertaken for the literature of iPSCs. The key words were “induced pluripotent stem cells, embryonic stem cells, somatic cells, reprogramming, induction, transcription factor”.
RESULTS AND CONCLUSION: iPSCs of specific genes, mainly through import, reprogram somatic cells. Its specific genes include Oct 4, Sox2, c-Myc, Klf4, Nanog, Lin-28. These exogenous transcription factors can activate endogenous Oct4 and Sox2 expression, which contribute to form a network of self-regulation and to maintain iPSCs pluripotent. However, the specific mechanism of reprogramming remains unclear.

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