中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (41): 7653-7656.doi: 10.3969/j.issn.1673-8225.2010.41.013

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

小鼠3T3-L1前脂肪细胞分化过程中过氧化物酶增殖物激活受体γ与CCAAT/增强子结合蛋白α的表达　

陈思凡，孙  健，郑  琳，张子丽，孙延双，冯  翔   

  1. 中山大学公共卫生学院，广东省广州市  510080
  • 出版日期:2010-10-08 发布日期:2010-10-08
  • 通讯作者: 冯翔，博士，副教授，硕士生导师，主要从事营养与健康方面的研究。 fengx@mail.sysu.edu.cn
  • 作者简介:陈思凡★，男，1984年生，广东省梅州市人，汉族，中山大学公共卫生学院在读硕士，主要从事营养与健康方面的研究。 dishi1984@126.com

Expressions of peroxisome proliferator-activated receptor gamma and CCAAT/enhancer binding protein alpha during the differentiation process of mouse 3T3-L1 preadipocytes

Chen Si-fan, Sun Jian, Zheng Lin, Zhang Zi-li, Sun Yan-shuang, Feng Xiang   

  1. School of Public Health, Sun Yat-sen University, Guangzhou   510080, Guangdong Province, China
  • Online:2010-10-08 Published:2010-10-08
  • Contact: Feng Xiang, Doctor, Associate professor, Master’s supervisor, School of Public Health, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China fengx@mail.sysu.edu.cn
  • About author:Chen Si-fan★, Studying for master’s degree, School of Public Health, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China dishi1984@126.com

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摘要：

背景：目前关于脂肪细胞分化的分子作用机制的研究较少。过氧化物酶增殖物激活受体和CCAAT/增强子结合蛋白家族的转录调控因子可诱导前脂肪细胞表达促进其分化成熟的多个转录因子，但其作用机制却罕见报道。
目的：观察小鼠3T3-L1前脂肪细胞分化为成熟脂肪细胞过程中过氧化物酶增殖物激活受体γ和CCAAT/增强子结合蛋白α的表达，以及在脂肪细胞分化过程中的变化。
方法：体外培养3T3-L1前脂肪细胞，采用经典激素鸡尾酒诱导法诱导细胞分化，诱导剂为1-甲基-3-异丁基-黄嘌呤、地塞米松和胰岛素。诱导后0，2，4，6和8 d，用油红O染色和染色比色法分析脂肪细胞的分化程度，采用实时PCR和Western blot技术检测不同时间点的过氧化物酶增殖物激活受体γ和CCAAT/增强子结合蛋白α的表达。
结果与结论：在前体脂肪细胞的分化过程中，随时间的延长相对脂肪含量、过氧化物酶增殖物激活受体γ与CCAAT/增强子结合蛋白α表达水平均明显升高(P < 0.01)。说明在小鼠3T3-L1前脂肪细胞分化为成熟脂肪细胞过程中，过氧化物酶增殖物激活受体γ与CCAAT/增强子结合蛋白α对细胞分化可能起促进作用。

关键词: 脂肪细胞, 细胞培养, 细胞分化, 过氧化物酶增殖物激活受体&gamma, CCAAT/增强子结合蛋白&alpha

Abstract:

BACKGROUND: Few studies were concerned about the molecular mechanisms in the process of adipocyte differentiation at present. The transcription factors of peroxisome proliferator-activated receptors (PPAR) and CCAAT/enhancer binding protein (C/EBP) family can induce and promote the differentiation, but the mechanisms were rarely reported.
OBJECTIVE: To investigate PPAR γ and C/EBP α expression during the process of murine 3T3-L1 preadipocytes differentiating into mature adipocytes, and explore the mechanisms in the differentiation process of adipocytes.
METHODS: Murine 3T3-L1 preadipocytes was cultured in vitro and induced by using the classic hormone-cocktail method (1-methyl-3-butyl-anthine + dexamethasone + insulin). At 0, 2, 4, 6 and 8 days following induction, oil red O staining method and spectrophotography were applied to analyze the differentiation of adipocytes. Real time polymerase chain reaction (PCR) and Western blot methods were applied to detect the expression of PPARγ and C/EBPα at different time points.
RESULTS AND CONCLUSION: In the process of cell differentiation, the difference of relative fat content, expression levels of PPARγ and C/EBPα were significantly increased (P < 0.01). These indicated that PPARγ and C/EBPα play a promoting effect in adipocyte differentiation from 3T3-L1 preadipocytes.

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