中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (34): 5033-5038.doi: 10.3969/j.issn.2095-4344.2016.34.003

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

新型可塑形同种骨修复材料修复运动性关节软骨损伤

丁晨光1,付 平2   

  1. 1河北中医学院,河北省石家庄市 050200;2河南护理职业学院,河南省安阳市 455001
  • 收稿日期:2016-05-25 出版日期:2016-08-19 发布日期:2016-08-19
  • 通讯作者: 付平,讲师,河南护理职业学院,河南省安阳市 455001
  • 作者简介:丁晨光,男,1979年生,河北省景县人,汉族,硕士,讲师,主要从事高校体育教育、运动医学研究。

A novel plastic homogeneous bone repair material for sport-related articular cartilage injury

Ding Chen-guang1, Fu Ping2   

  1. 1Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei Province, China; 2Henan Vocational College of Nursing, Anyang 455001, Henan Province, China
  • Received:2016-05-25 Online:2016-08-19 Published:2016-08-19
  • Contact: Fu Ping, Lecturer, Henan Vocational College of Nursing, Anyang 455001, Henan Province, China
  • About author:Ding Chen-guang, Master, Lecturer, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei Province, China

摘要:

文章快速阅读:

 

文题释义:
新型可塑形同种骨修复材料
:新型可塑形同种骨修复材料由成骨基质颗粒、黏性载体组成,能为机体提供一定的力学性能。此外,新型可塑形同种骨修复材料以液态、泥膏状或含有颗粒状材料运用于凝胶剂,置入时切口相对较小,具备良好的组织相容性和骨传导能力。黏性载体则是将松散的成骨基质颗粒黏合在一起,使材料整体具备可塑性,提高材料的整体性能,发挥良好的骨修复作用。
新型可塑形同种骨修复材料的骨修复能力:与脱钙骨基质在材料中的比例关系密切。通常而言,脱钙骨基质含量较高的材料骨修复能力优于含量较低者。目前,临床上应用的可塑形同种骨修复材料主要以硫酸钙、甘油、高分子量水凝胶等为黏性载体,该材料在制备过程中使用的所有的组分均来自同种骨组织,能够有效提高材料中的脱钙骨基质颗粒含量,保证其含量在90%以上。


背景:目前临床上对于可塑形骨修复材料的基本组成及制备方法存在较大争议,且对于其在运动性关节软骨损伤中的修复效果尚缺乏报道。
目的:分析新型可塑形同种骨修复材料修复运动性关节软骨损伤的效果。
方法:取36只新西兰大白兔,建立关节软骨损伤模型后,随机分为2组,实验组采用新型可塑形同种骨修复材料(为脱钙骨基质与骨胶原复合物)修复,对照组采用透明质酸钠凝胶修复,修复后3周,采用动物实验跑台模拟运动员受伤后的运动训练,30 min/次,连续运动5次。修复后16,18,20周,进行缺损部位组织学观察。
结果与结论:①修复后16周,实验组缺损部位修复良好,周围未见明显炎症反应,且与周围组织出现融合;对照组修复部位存在一裂隙,材料与间质部位相互融合,修复部位周围未见炎症反应;②修复后18周,实验组损伤部位可见排列紧密的脱细胞骨基质颗粒,纤维结缔组织相对较少,宿主骨边缘存在新骨;对照组损伤部位愈合,但新骨相对较少;③修复后20周,实验组损伤部位被纤维结缔组织填充,纤维结缔组织内存在大量的血管长入,周围宿主骨边缘可见少量新生组织;对照组损伤部位得到改善,但未见新生血管;④结果表明,新型可塑形同种骨修复材料可促进运动性关节软骨损伤的修复且排斥反应小。

ORCID: 0000-0002-1154-2126(丁晨光)

关键词: 生物材料, 软骨生物材料, 新型可塑性同种骨修复材料, 运动性关节软骨损伤, 修复效果, 透明质酸钠凝胶, Wakitani评分, 炎症反应

Abstract:

BACKGROUND: Currently, the components and preparation methods of plastic bone repair materials remain controversial, and rare studies focus on their repair effects on sport-related articular cartilage injury.
OBJECTIVE: To analyze the effect of a novel plastic homogeneous bone repair material to repair sport-related articular cartilage injury.
METHODS: Thirty-six New Zealand rabbits were selected to prepare articular cartilage injury models, and were randomized into two groups. Model rabbits were repaired with the novel plastic homogeneous bone material (a composite of demineralized bone matrix and collagen) as experimental group, while the others repaired with sodium hyaluronate gel as control group. At 3 weeks after repair, treadmill system was utilized to stimulate sports training after athlete injury, for five consecutive sessions of 30 minutes each. At 16, 18 and 20 weeks after repair, the defect region was observed histologically.
RESULTS AND CONCLUSION: At 16 weeks after repair, the defect region healed well and integrated with the surrounding tissues, and no significant inflammatory reaction appeared around the material in the experimental group; in the control group, a fissure appeared at the defect region but with no inflammatory reaction, and the material integrated with the interstitial site. At 18 weeks after repair, closely arranged acellular bone matrix could be found in the defect region, fibrous connective tissues were fewer, and there was a new bone formation around the edge of host bone in the experimental group; the defect region healed, but new bones were fewer in the control group. At 20 weeks after repair, the defect region in the experimental group was filled with fibrous connective tissues, in which there were numerous new blood vessels, and a few of new tissues appeared around the edge of host bone; the defect region in the control group was improved and no neovascularization occurred. These findings suggest that the novel plastic homogeneous bone material can promote the repair of sport-related articular cartilage injury with less rejection reactions.

 

中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程

Key words: Collagen, Hyaluronic Acid, Tissue Engineering

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