中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (46): 7445-7450.doi: 10.3969/j.issn.2095-4344.2015.46.013

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

止血带致肢体缺血再灌注损伤时炎性递质变化及缺血预处理的作用

闫 斌，哈斯鲁，买合木提•亚库甫，侯彦杰，徐 超，伊力哈木•托合提   

  1. 新疆医科大学第二附属医院，新疆维吾尔自治区乌鲁木齐市 830063
  • 收稿日期:2015-10-09 出版日期:2015-11-12 发布日期:2015-11-12
  • 通讯作者: 伊力哈木?托合提，博士，主任医师，新疆医科大学第二附属医院，新疆维吾尔自治区乌鲁木齐市 830063
  • 作者简介:闫斌，男，1978年生，新疆维吾尔自治区乌鲁木齐市人，主治医师，主要从事四肢骨折、运动损伤的治疗研究。
  • 基金资助:

    新疆医科大学2013年科研创新基金项目(XJC201368)

Inflammatory transmitter changes during tourniquet-induced limb ischemia-reperfusion injury and the role of ischemic preconditioning

Yan Bin, Hasilu, Maihemuti Yakufu, Hou Yan-jie, Xu Chao, Yilihamu Tuoheti   

  1. Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China
  • Received:2015-10-09 Online:2015-11-12 Published:2015-11-12
  • Contact: Yilihamu Tuoheti, M.D., Chief physician, Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China
  • About author:Yan Bin, Attending physician, Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830063, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the Scientific Research and Innovation Fund of Xinjiang Medical University in 2013, No. XJC201368

PDF

186

被引次数

6

摘要：

背景：血清白细胞介素6和白细胞介素8的过度释放可能参与止血带所致的肢体缺血再灌注性肺换气功能损伤。
目的：分析安全时限内止血带应用于大鼠下肢对其血清白细胞介素6、白细胞介素8的影响。
方法：将60只大鼠随机分为对照组和实验组，各30只。对照组未经过缺血预处理，直接止血2，3，4 h；实验组经过缺血预处理(在诱发缺血再灌注损伤前提前1 d多次短时间止血)后，于第2天分别止血2，3，4 h。依次于恢复肢体血流(再灌注)后1，3，7，14 d时抽取血液，采用ELISA法测试大鼠血液中白细胞介素6、白细胞介素8的水平。
结果与结论：两组血清中白细胞介素6、白细胞介素8的水平呈递增递减的趋势，且均在第3天时达峰值(P < 0.05)；实验组大鼠血液白细胞介素6在中第1，3，7，14天显著低于对照组(P < 0.05)，仅在第7天实验组中止血3 h的大鼠血清白细胞介素6高于对照组(P < 0.05))；在第7天实验组中止血3 h的大鼠血清中白细胞介素8水平低于对照组，但差异无显著性意义(P > 0.05)，在第3，7，14天时，止血4 h的大鼠血清中白细胞介素8的浓度高于对照组(P < 0.05)；实验组大鼠第1、3天内血清中白细胞介素6及白细胞介素8的水平随着止血时间延长而增高(2 h < 3 h < 4 h)，差异有显著性意义，而对照组大鼠血清中白细胞介素6、白细胞介素8的水平在第1、3天内有随止血时间延长而增高的趋势，但差异无统计学意义。结果说明在大鼠诱发肢体再灌注损伤时，大鼠体内炎症反应在第3天时最明显，给予大鼠止血带预处理，可显著减轻体内的炎性反应，肢体缺血再灌注时，缺血时间越长，体内的炎性反应越明显。
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 组织构建, 组织工程, 缺血预处理, 缺血再灌注损伤, 止血带, 炎性递质

Abstract:

BACKGROUND: Over-expression of serum interleukin-6 and interleukin-8 may be involved in tourniquet-induced limb ischemia-reperfusion injury to the lung ventilation function.

OBJECTIVE: To evaluate the tourniquet effect on serum interleukin-6 and interleukin-8 levels of the rat limb within the safety time limit.
METHODS: Sixty rats were randomly divided into control and experimental groups, 30 rats in each group. Rats in the control group had no ischemic preconditioning and were directly subjected to stop bleeding for 2, 3, 4 hours; rats in the experimental group were subjected to ischemic preconditioning (short-time hemostasis for several times within 1 day before ischemia-reperfusion injury), and then underwent 2-, 3-, 4-hour hemostasis at the 2nd day. At 1, 3, 7, 14 days after the recovery of limb blood flow, blood samples were extracted to detect serum interleukin-6 and interleukin-8 levels using ELISA method.
RESULTS AND CONCLUSION: The levels of interleukin-6 interleukin-8 showed an increasing and decreasing trend in the two groups, which both reached the peak at the 3rd day (P < 0.05). The levels of interleukin-6 in the experimental group was significantly higher than that in the control group at 1, 3, 7, 14 days (P < 0.05), and only at 7 days, the level of interleukin-6 in the rats undergoing 3-hour hemostasis was significantly lower than that in the control group (P < 0.05). At 7 days, the level of interleukin-8 in the rats undergoing 3-hour hemostasis was lower than that in the control group, but there was no significant difference (P > 0.05), and at 3, 7, 14 days, the level of interleukin-8 in the rats undergoing 4-hour hemostasis was significantly higher than that in the control group (P < 0.05). At 1 and 3 days, the levels of interleukin-6 and interleukin-8 in the experimental group had an increasing trend with the bleeding time and ranked as follows: 2-hour hemostasis < 3-hour hemostasis < 4-hour hemostasis, and there was a significant difference; while in the control group, there was also an increasing trend in the levels of interleukin-6 and interleukin-8, but there was no statistical difference. These findings indicate that the tourniquet preconditioning treatment is preferred at 3 days after limb ischemia-reperfusion injury, when the inflammatory response was the most obvious in rats, and this treatment can dramatically reduce inflammatory response. Additionally, the inflammatory become more obvious with the bleeding time.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Ischemia, Ischemic Preconditioning, Reperfusion Injury