中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (28): 4478-4484.doi: 10.3969/j.issn.2095-4344.2015.28.010

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

间充质干细胞对哮喘模型小鼠的免疫调节作用：局部与全身应用效果不同

姚 银1，2，郭洁波1，邓学泉1，2，孙悦奇1，2，付清玲1，2   

  1. 1中山大学附属第一医院耳鼻咽喉科医院，广东省广州市 510080；
    2中山大学耳鼻咽喉科研究所，广东省广州市  510080

Intravenous versus intratracheal administration of mesenchymal stem cells in a mouse model of asthma 

Yao Yin1, 2, Guo Jie-bo1, Deng Xue-quan1, 2, Sun Yue-qi1, 2, Fu Qing-ling1, 2   

  1. 1Otorhinolaryngology Hospital, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; 
    2Otorhinolaryngology Institute, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Online:2015-07-02 Published:2015-07-02
  • Contact: Fu Qing-ling, M.D., Researcher, Otorhinolaryngology Hospital, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; Otorhinolaryngology Institute, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • About author:Fu Qing-ling, M.D., Researcher, Otorhinolaryngology Hospital, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; Otorhinolaryngology Institute, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
  • Supported by:

    the National Natural Science Foundation of China, No. 81170896; the Science and Technology Foundation of Guangdong Province, No. 2012B031800045

    中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

PDF

298

摘要：

背景：研究显示间充质干细胞移植可以减轻呼吸道变应性炎症的相关症状，然而尚无不同移植途径下间充质干细胞对哮喘疗效的比较。

目的：观察间充质干细胞不同应用方式对小鼠哮喘模型的免疫调节作用的区别。
方法：Balb/c小鼠72只进行3次独立的实验，每次24只。将24只小鼠按随机数字表法等分为对照组、模型组、全身应用组和局部应用组。模型组、全身应用组和局部应用组小鼠分别在第1，7，14天以进行3次卵清蛋白腹腔注射致敏，第22-26天卵清白蛋白溶液持续雾化吸入30 min，连续激发5 d。全身应用组和局部应用组小鼠于雾化前1 d分别经尾静脉注入200 μL间充质干细胞混悬液(5×109 L-1)和气管注入50 μL间充质干细胞混悬液(2×1010 L-1)。
结果与结论：与正常组相比，模型组小鼠气道炎症反应显著增强，间充质干细胞的全身应用可以下调气道高反应，降低肺泡灌洗液中炎症细胞数量，减轻肺组织中的炎症浸润，抑制血清中卵清白蛋白特异性IgE的分泌，降低肺泡灌洗液中Th2型细胞因子的水平；而间充质干细胞的局部应用对上诉症状无改善。间充质干细胞的全身应用可有效减轻哮喘小鼠的气道炎症反应，而其局部应用无改善作用。提示间充质干细胞不适合采用经气管内注射的应用方式，可能需经全身血循环发挥免疫调节作用。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 干细胞, 骨髓干细胞, 哮喘, 间充质干细胞, 卵清蛋白, 全身应用, 局部应用, 炎症, 气道炎症反应, 国家自然科学基金

Abstract:

BACKGROUND: Several studies have demonstrated that mesenchymal stem cells exhibit strong immunomodulation on allergic asthma. However, there are no reports to compare therapeutic effects under different administration ways.

OBJECTIVE: To examine the immunomodulatory effects of MSCs via different administration routes on asthmatic mice.
METHODS: Seventy-two Balb/c mice experienced three independent tests, and 24 mice were selected for each test. Twenty-four mice were randomly divided into four groups (n=6): control group, model group, intravenous treatment group and intratracheal treatment group. The mouse model of asthma was induced via intraperitoneal injection of ovalbumin at 1, 7, 14 days and 30-minute aerosol inhalation of ovalbumin at 22-26 days. In the latter two groups, mesenchymal stem cells were injected intravenously (200 μL, 5×109/L) or intratracheally (50 μL, 2×109/L) into the mice at 1 day before aerosol inhalation.

RESULTS AND CONCLUSION: Compared with the control group, the airway inflammatory response was significantly increased in the model group. Intravenous administration of mesenchymal stem cells significantly alleviated the symptoms of allergic airway inflammation, including the airway hyperreactivity, the inflammatory cell counting in the bronchoalveolar lavage fluid, inflammatory cell infiltration in the lung tissue. Meanwhile, the levels of Th2 type cytokines in the bronchoalveolar lavage fluid and IgE in serum also decreased after intravenous administration of mesenchymal stem cells. However, the intratracheal application of mesenchymal stem cells did not exhibit the similar effects. Intravenous, not intratracheal, application of mesenchymal stem cells can exert immunomodulatory effects through the blood circulation. 
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Asthma, Mesenchymal Stem Cells, Inflammation, Trachea

中图分类号: