中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (20): 3218-3222.doi: 10.3969/j.issn.2095-4344.2014.20.019

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

不同浓度人羊膜匀浆上清液培养大鼠许旺细胞的增殖

刘  亮1，王  磊1，童亚林2，莫永亮1，吕  璐1，陈云鹏1，杨文贤3，吕礼芳3，詹  球3，朱富军2，辛海明2，龚震宇2   

  1. 1广西师范大学研究生培养基地暨解放军第181医院烧伤整形科，广西壮族自治区桂林市  541002；解放军第181医院，2烧伤整形科，3实验研究中心、广州军区烧伤整形中心实验室，广西壮族自治区桂林市  541002
  • 收稿日期:2014-04-03 出版日期:2014-05-14 发布日期:2014-05-14
  • 通讯作者: 童亚林，主任医师，解放军第181医院烧伤整形科，广西壮族自治区桂林市 541002
  • 作者简介:刘亮，男，1987年生，湖南省衡阳市人，汉族，广西师范大学生命科学学院在读硕士，主要从事重大疾病分子机制方面的研究。
  • 基金资助:

    广西科学研究与技术开发计划项目(1140003A-39)；广西自然科学基金(0991290)；广西自然科学基金(2011GXNSFB018107)；国家自然科学基金青年科学基金项目(81301634)；全军医学科学技术研究“十二五”计划课题项目(CWS11J277)

Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells

Liu Liang1, Wang Lei1, Tong Ya-lin2, Mo Yong-liang1, Lv Lu1, Chen Yun-peng1, Yang Wen-xian3, Lv Li-fang3, Zhan Qiu3, Zhu Fu-jun2, Xin Hai-ming2, Gong Zhen-yu2   

  1. 1Department of Burn and Plastic Surgery, the 181 Hospital of Chinese PLA, Postgraduate Cultivation Basement of Guangxi Normal University, Guilin  541002, Guangxi Zhuang Autonomous Region, China; 2Department of Burn and Plastic Surgery, the 181 Hospital of Chinese PLA, Guilin 541002, Guangxi Zhuang Autonomous Region, China; 3Experimental Research Center, the 181 Hospital of Chinese PLA, Central Laboratory of Burn and Plastic Surgery, Guangzhou Military Region of Chinese PLA, Guilin 541002, Guangxi Zhuang Autonomous Region, China
  • Received:2014-04-03 Online:2014-05-14 Published:2014-05-14
  • Contact: Tong Ya-lin, Chief physician, Department of Burn and Plastic Surgery, the 181 Hospital of Chinese PLA, Guilin 541002, Guangxi Zhuang Autonomous Region, China
  • About author:Liu Liang, Studying for master’s degree, Department of Burn and Plastic Surgery, the 181 Hospital of Chinese PLA, Postgraduate Cultivation Basement of Guangxi Normal University, Guilin 541002, Guangxi Zhuang Autonomous Region, China
  • Supported by:

    Science Research and Technology Development Project of Guangxi Zhuang Autonomous Region, No. 1140003A-39; Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. 0991290, 2011GXNSFB018107; National Natural Science Foundation of China for Youths, No. 81301634; Military Medical Science Research Project During the Twelfth Five-Year Plan, No. CWS11J277

PDF

564

被引次数

9

摘要：

背景：许旺细胞是周围神经修复过程的重要细胞，而研究发现人羊膜细胞分泌的多种细胞因子能够促进许旺细胞增殖。
目的：观察不同浓度人羊膜匀浆上清液对鼠许旺细胞(RSC96)生长的影响。
方法：使用含体积分数20%胎牛血清的高糖DMEM培养基原代培养RSC96细胞株，传代至第2代用于实验研究。根据人羊膜匀浆上清液在培养基中的不同体积分数(0，10，15，20，25%)分组。
结果与结论：人羊膜匀浆上清液的总蛋白浓度为675 mg/L，表皮生长因子、碱性成纤维生长因子和血管内皮生长因子浓度分别为(470.625±2.546)，(4.121±0.026)和(0.172±0.002) ng/L。在培养第1-7天，10%和15%人羊膜匀浆上清液组的增殖率大于20%和25%人羊膜匀浆上清液组(P < 0.05)；10%、15%人羊膜匀浆上清液组显示出促进细胞增殖的作用，而20%、25%人羊膜匀浆上清液组显示出抑制细胞增殖的作用；各实验组的细胞活力与对照组接近(P > 0.05)。提示人羊膜匀浆上清液低浓度时(10%和15%)具有促进RSC96增殖作用，高浓度时(20%和25%)抑制RSC96增殖。



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 组织工程, 人羊膜匀浆上清液, 许旺细胞, 细胞增殖, 细胞活力, 周围神经, 细胞生长曲线, MTT法, 国家自然科学基金

Abstract:

BACKGROUND: Schwann cells are important cell lines in the process of repairing peripheral nerve injury, and human amniotic homogenate supernatant is shown to secrete a variety of cytokines, which could promote the proliferation of Schwann cells.
OBJECTIVE: To investigate the effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cell 96.
METHODS: Schwann cell 96 was cultured with high-glucose DMEM containing 20% fetal bovine serum, and the second generation of Schwann cell 96 was applied for experiments. The cultured cells were divided into five groups according to different volume fractions of human amniotic homogenate supernatant (0%, 10%, 15%, 20%, 25%) in the medium. 
RESULTS AND CONCLUSION: The total protein concentration of human amniotic homogenate supernatant was
675 μg/mL, in which the concentration of epidermal growth factor, basic fibroblast growth factor and vascular endothelial growth factor were respectively (470.625±2.546), (4.121±0.026) and (0.172±0.002) ng/L. At 1-7 days, the cell proliferation rate of the 10% and 15% concentration groups was greater than that in 20% and 25% concentration groups (P < 0.05); 10% and 15% concentrations promoted cell proliferation, while 20% and 25% concentrations inhibited cell proliferation. There were no significant difference in the viability of Schwann cell 96 between the control group and the experimental group (P > 0.05). Low concentrations (10%, 15%) of human amniotic homogenate supernatant promote the proliferation of Schwann cell 96, while high concentrations (20%, 25%) of human amniotic homogenate supernatant inhibit cell proliferation.



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: amnion, Schwann cells, cell proliferation, cytokines

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