中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (8): 1185-1190.doi: 10.3969/j.issn.2095-4344.2014.08.007

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

羟基丁酸-羟基辛酸一体化骨软骨支架的体外血管化

肖  威1，任  玮1，张永红1，赵良启2   

  1. 1山西医科大学第二医院骨科，山西省太原市  030001；2山西大学生物技术研究所，山西省太原市  030006
  • 收稿日期:2013-12-02 出版日期:2014-02-19 发布日期:2014-02-19
  • 通讯作者: 张永红，博士，硕士生导师，主任医师，山西医科大学第二医院骨科，山西省太原市 030001
  • 作者简介:肖威，男，1988 年生，湖北省孝感市人，汉族，山西医科大学第二医院在读硕士，主要从事骨与软骨组织工程的研究。
  • 基金资助:

    国家自然科学基金(主任基金)项目(31040030)，项目名称：新型多聚羟基烷酸软骨组织工程载体的研究

Vascularization of poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold in vitro

Xiao Wei1, Ren Wei1, Zhang Yong-hong1, Zhao Liang-qi2   

  1. 1Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; 2Institute of Biotechnology, Shanxi University, Taiyuan 030006, Shanxi Province, China
  • Received:2013-12-02 Online:2014-02-19 Published:2014-02-19
  • Contact: Zhang Yong-hong, M.D., Master’s supervisor, Chief physician, Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • About author:Xiao Wei, Studying for master’s degree, Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31040030

PDF

488

被引次数

1

摘要：

背景：前期实验构建的羟基丁酸-羟基辛酸共聚体一体化骨软骨支架具备良好的生物相容性、生物可降解性，并且降解产物无毒性。
目的：将兔肾微血管内皮细胞与羟基丁酸-羟基辛酸一体化骨软骨支架复合培养，观察支架骨层血管化效果。
方法：运用溶剂浇铸-颗粒沥滤法，制备具有骨层/骨与软骨界面层/软骨层3层结构的羟基丁酸-羟基辛酸一体化骨软骨支架。将传代培养至第3 代的兔肾微血管内皮细胞，接种到一体化骨软骨支架骨层支架上，MTT法检测细胞在支架上的增殖活性，10 d后苏木精-伊红染色及电镜观察细胞在支架内的生长状况。
结果与结论：一体化骨软骨支架外观具备明显的3层结构，各层之间连接紧密，骨层疏松多孔，各层支架孔隙均匀且相通，一体化支架孔隙率为78%。兔肾微血管内皮细胞在支架上分裂增殖良好，复合培养10 d后，细胞在骨层支架内呈立体生长，中间界面层内未发现细胞，苏木精-伊红染色可见细胞黏附生长于骨层支架孔隙间，细胞依附支架的多孔结构生长，形成管腔样结构，但细胞并未长入中间界面层。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 骨生物材料, 软骨生物材料, 羟基丁酸-羟基辛酸共聚体, 骨软骨, 一体化支架, 组织工程, 血管化, 国家自然科学基金

Abstract:

BACKGROUND: The poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold which has been constructed in previous experiments has good biocompatibility and biodegradability and generates non-toxic degradation products.
OBJECTIVE: To observe the vascularization of rabbit renal microvascular endothelial cells co-cultured with poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold.
METHODS: The poly(hydroxybutyrate-co-hydroxyoctanoate) osteochondral scaffold having a three-layer structure (layer of bone/bone and cartilage interface layer/layer of cartilage) was prepared by solvent casting/particle leaching method. The renal microvascular endothelial cells at passage 3 were seeded onto the scaffold of bone layer. The proliferation of the renal microvascular endothelial cells growing on the scaffolds was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method, the growth of cells in the scaffold was observed by hematoxylin-eosin staining under electron microscope after 10 days.
RESULTS AND CONCLUSION: The integrated osteochondral scaffold had a clear appearance of three-layer structure, which had closed connections between the three layers. Porous bone layer was visible as well as uniform and interlinked pores, and the porosity was 78%. The renal microvascular endothelial cells seeded onto the scaffold proliferated well and presented a three-dimensional growth after 10 days of co-culture, but there were no cells on the interface layer. Cells which adhered and grew between the pores of the bone layer were observed through hematoxylin-eosin staining. Cells showed a luminal-like structure growing on the scaffold with the porous structure, but they did not grow into the interface layer of bone and cartilage.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: biocompatible materials, neovascularization, physiologic, 3-hydroxybutyric acid, tissue engineering

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