中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (43): 8049-8054.doi: 10.3969/j.issn.2095-4344.2012.43.016

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

血管组织工程支架材料的细胞相容性

肖荣冬1，翁国星2   

  1. 1福建医科大学省立临床学院心外科，福建省福州市 350001
    2福建省立医院心血管外科，福建省福州市 350001
  • 收稿日期:2012-01-07 修回日期:2012-03-12 出版日期:2012-10-21 发布日期:2012-10-21
  • 作者简介:肖荣冬☆，男，1973年生，福建省武平县人，汉族，2006年福建医科大学毕业，博士，副主任医师，主要从事组织工程血管研究。 xrddoctor@ yahoo.com.cn

Cytocompatibility of the scaffold materials in vascular tissue engineering

Xiao Rong-dong1, Weng Guo-xing2   

  1. 1Deparment of Heart Surgery, Provincial Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China
    2Department of Cardiovascular Surgery, Fujian Provincial Hospital, Fuzhou 350001, Fujian Province, China
  • Received:2012-01-07 Revised:2012-03-12 Online:2012-10-21 Published:2012-10-21
  • About author:Xiao Rong-dong☆, Doctor, Associate chief physician, Department of Heart Surgery, Provincial Clinical College of Fujian Medical University, Fuzhou 350001, Fujian Province, China xrddoctor@ yahoo.com.cn

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摘要：

背景：胶原与透明质酸均有利于组织培养中细胞的黏附、增殖和分化。
目的：观察血管内皮细胞、平滑肌细胞与胶原/透明质酸膜、明胶海绵的细胞相容性，并筛选最佳种植方法。
方法：将第3-5代兔血管平滑肌细胞种植在胶原/透明质酸膜(或明胶海绵)材料上，连续培养2周后将兔内皮细胞接种在平滑肌细胞-胶原/透明质酸膜(或明胶海绵)复合体上，并设置单纯平滑肌细胞与内皮细胞共同接种组。
结果与结论：①光镜和扫描电镜观察：细胞在两种材料上均随着培养时间，接种次数增加而生长加快，其中在胶原/透明质酸膜上的细胞生长更好，细胞连接更致密。②WST-1法检测：胶原/透明质酸膜组平滑肌细胞的黏附率及增殖率均高于明胶海绵组(P < 0.05)，且细胞在材料上的生长随着接种次数的增加有不同程度提高。③3H-TDR掺入法检测DNA合成率：在胶原/透明质酸膜上的细胞DNA合成最高，明胶海绵上的较差。表明胶原/透明质酸膜具有较理想的细胞相容性，采用适当间隔、反复接种的方法可提高细胞的黏附和增殖。

关键词: 胶原, 透明质酸膜, 平滑肌细胞, 细胞相容性, WST-1, H-TDR组织工程补片, 生物材料

Abstract:

BACKGROUND: Collagen and hyaluronic sacid are both helpful to cell adhesion, proliferation and differentiation in the tissue culture.
OBJECTIVE: To investigate the cytocompatibility between the three scaffolds (collagen, hyaluronic acid membrane, and latin sponge) and vascular endothelial cells, smooth muscle cells, as well as to screen the optimal seeding method.
METHODS: The passage 3-5 vascular smooth muscle cells of rabbits were seeded onto the collagen/hyaluronic acid membrane (or latin sponge); after cultured for 2 weeks, rabbit vascular endothelial cells were seeded onto the compound of smooth muscle cells-collagen/hyaluronic acid membrane (or latin sponge). Pure endothelial cells and smooth muscle cells were performed inoculation together.
RESULTS AND CONCLUSION: ①Light microscope and scanning electron microscope results showed that the inoculation times and growth were increased with culture time, and the cells on the collagen/hyaluronic acid membrane grew better, what’s more, cells attached more tightly. ②WST-1 method results showed that the adhesion rate and proliferation rate of smooth muscle cells in the collagen/hyaluronic acid membrane group were both higher than the latin sponge group (P < 0.05). Besides, cells growth on the scaffolds was increased in various degrees. ③3H-TDR method results indicated that DNA synthesis of cells in the collagen/hyaluronic acid membrane group was highest, and that in the latin sponge group was lower. These findings suggest that collagen/hyaluronic acid membrane has ideal cytocompatibility. Repeating seeding with proper interval can increase the adhesion and proliferation of cells on scaffolds.

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