中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7893-7896.doi: 10.3969/j.issn.2095-4344.2012.42.020

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

线粒体酶活性及线粒体肿胀与不同运动强度的关系

徐 莉1，曹 颖2，张 敏3   

  1. 1天津体育学院，天津市300381
    2 天津中医药大学第一附属医院检验科，天津市 300193
    3武警后勤学院生化教研室，天津市 300162
  • 收稿日期:2012-01-15 修回日期:2012-02-27 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 张敏，硕士研究生，武警后勤学院生化教研室，天津市 300162 zhangmin53421@163.com
  • 作者简介:徐莉★，女，1967年生，北京市人，汉族，1996年天津体育学院毕业，硕士，主要从事体育教育训练学专业方面的研究。 felice@sina.com

Effects of different exercise intensities on the activity of mitochondrial enzymes and mitochondrial swelling

Xu Li1, Cao Ying2, Zhang Min3   

  1. 1Tianjin Institute of Physical Education, Tianjin 300381, China
    2Clinical Laboratory, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
    3Biochemistry Department, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China
  • Received:2012-01-15 Revised:2012-02-27 Online:2012-10-14 Published:2012-10-14
  • Contact: Zhang Min, Studying for master’s degree, Biochemistry Department, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300162, China zhangmin53421@163.com
  • About author:Xu Li★, Master, Tianjin Institute of Physical Education, Tianjin 300381, China felice@sina.com

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571

被引次数

5

摘要：

背景：关于不同运动强度对机体的影响早有研究，并且取得了不少有价值的成果。但是对于不同运动强度对线粒体Na+,K+-ATP酶、Ca2+-ATP酶活性以及线粒体肿胀程度影响的研究较少。
目的：观察不同运动强度对大鼠骨骼肌线粒体Na+，K+-ATP酶和Ca2+-ATP酶以及线粒体肿胀的影响。
方法：将24只SD大鼠随机分为正常对照组，中等强度运动组和高强度运动组。正常对照组大鼠正常笼内生活，不运动。另2组游泳训练方式，分别建立中强度和高强度运动模型，进行相应的运动锻炼，训练4周。
结果与结论：中强度运动组线粒体Na+,K+-ATP酶、Ca2+-ATP酶活性与正常对照组相比均增高(P < 0.05)，线粒体肿胀程度降低(P < 0.05)；而高强度运动组线粒体Na+,K+-ATP酶、Ca2+-ATP酶活性与正常对照组相比均降低(P < 0.05)，线粒体肿胀程度增大(P < 0.05)。实验结果表明中强度运动可以保护线粒体Na+,K+-ATP酶、Ca2+-ATP酶的活性，提高线粒体功能；而高强度运动则降低了线粒体的功能。

关键词: 运动强度, 线粒体, Na+, K+-ATP酶, Ca2+-ATP酶, 线粒体肿胀, 组织构建

Abstract:

BACKGROUND: There are many studies on the effects of different exercise intensities on the body, but studies about the effects of different exercise intensities on the activity of mitochondrial Na+, K+-adenosine triphosphatase (ATPase), Ca2+-ATPase and mitochondrial swelling are few.
OBJECTIVE: To investigate the effects of different exercise intensities on the activity of mitochondrial Na+, K+-ATPase and Ca2+-ATPase, as well as mitochondrial swelling.
METHODS: Twenty-four Sprague Dawley rats were randomly separated into three groups, namely, control, mid-intensity, high-intensity exercise groups. The control rats did not undergo swimming training, while the other two groups were given 4-week swimming exercises of different intensities.
RESULTS AND CONCLUSION: Compared with the control group, the Na+, K+-ATPase and Ca2+-ATPase activities increased (P < 0.05) and the extent of mitochondrial swelling decreased (P < 0.05) in the mid-intensity exercise group, but opposite in the high-intensity exercise group. These findings indicate that mid-intensity exercise can protect the activity of mitochondrial Na+, K+-ATPase and Ca2+-ATPase and increase the function of mitochondria, but high-intensity exercise can decrease the function of mitochondria.

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