中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (42): 7814-7818.doi: 10.3969/j.issn.2095-4344.2012.42.005

• 骨组织构建 bone tissue construction • 上一篇    下一篇

环氧化酶2、骨形态形成蛋白2和血管内皮生长因子在异位骨化组织中的表达

田 建1，范存义2，芮永军1，靡菁熠1，阮洪江2，刘 珅2，曾炳芳2   

  1. 1无锡市第九人民医院，无锡市手外科医院手外科，江苏省无锡市 214064
    2上海交通大学附属第六人民医院骨科，上海市 200233
  • 收稿日期:2012-01-13 修回日期:2012-03-11 出版日期:2012-10-14 发布日期:2012-10-14
  • 通讯作者: 范存义，博士，主任医师，上海交通大学附属第六人民医院骨科，上海市 200233 fancunyi888@hotmail.com
  • 作者简介:田建★，男，1981年生，山东省临沂市人，汉族，2010年上海交通大学毕业，硕士，主治医师，主要从事创伤骨科和修复重建研究。 tianjian828@163.com

Expressions of cyclooxygenase 2, bone morphogenetic protein 2 and vascular endothelial growth factor in elbow heterotopic ossification

Tian Jian1, Fan Cun-yi2, Rui Yong-jun1, Mi Jing-yi1, Ruan Hong-jiang2, Liu Kun2, Zeng Bing-fang2   

  1. 1Department of Hand Surgery, the 9th People’s Hospital of Wuxi, Wuxi 214061, Jiangsu Province, China
    2Deprtment of Orthopedics, the 6th People’s Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 200233, China
  • Received:2012-01-13 Revised:2012-03-11 Online:2012-10-14 Published:2012-10-14
  • Contact: Fan Cun-yi, Doctor, Chief physician, Department of Orthopedics, the 6th People’s Hospital of Shanghai, Shanghai Jiao Tong University, Shanghai 200233, China fancunyi888@hotmail.com
  • About author:Tian Jian★, Master, Attending physician, Department of Hand Surgery, the 9th People’s Hospital of Wuxi, Wuxi 214061, Jiangsu Province, China tianjian828@163.com

PDF

467

被引次数

5

摘要：

背景：研究发现环氧化酶2抑制剂具有预防肘关节周围异位骨化的作用。
目的：观察环氧化酶2、骨形态形成蛋白2和血管内皮生长因子在肘关节创伤后异位骨化组织中的表达，并分析其相关性。
方法：采用免疫组化SP法检测18例肘关节创伤后异位骨化组织及10例正常骨组织中环氧化酶2、骨形态形成蛋白2和血管内皮生长因子的表达水平，利用HPIAS-1000图像分析系统测定异位骨化组织与正常骨组织中环氧化酶2、骨形态形成蛋白2和血管内皮生长因子的平均吸光度和阳性区域面积百分率，并分析3种蛋白阳性区域面积百分率之间的相关性。
结果与结论：异位骨化组织中环氧化酶2、骨形态形成蛋白2和血管内皮生长因子呈高表达；正常骨组织中呈低表达或不表达。图像分析结果显示异位骨化组织中3种蛋白平均吸光度及阳性区域面积百分率显著高于正常骨组织(P < 0.01)。异位骨化组织中环氧化酶2与骨形态形成蛋白2、血管内皮生长因子的阳性区域面积百分率呈正相关(P < 0.01)。提示环氧化酶2、骨形态形成蛋白2和血管内皮生长因子在异位骨化的形成过程中起重要作用，环氧化酶2可能通过诱导骨形态形成蛋白2和血管内皮生长因子的表达从而促进异位骨化组织中的成骨和血管形成。

关键词: 异位骨化, 肘关节, 免疫组化, 环氧化酶2, 骨形态形成蛋白2, 血管内皮生长因子, 组织工程

Abstract:

BACKGROUND: Several studies have shown that cyclooxygenase 2 (COX-2) inhibitors can prevent heterotopic ossification around the elbow joint.
OBJECTIVE: To investigate the expression of COX-2, bone morphogenetic protein 2 (BMP-2) and vascular endothelial growth factor (VEGF) in post-traumatic elbow heterotopic ossification tissues, and to analyze their relationship.
METHODS: The expressions of COX-2, BMP-2 and VEGF were examined by S-P immunohistochemical staining in 18 cases of heterotopic ossification and 10 cases of normal bone tissue. The 10 cases were as controls. The average absorbance value and the percentage of positive area of COX-2, BMP-2 and VEGF in heterotopic ossification and normal bone tissue were measured by HPIAS-1000 image analysis system. The relationship among the percentage of the positive area in the three kinds of proteins was analyzed.
RESULTS AND CONCLUSION: COX-2 ,BMP-2 and VEGF were strongly expressed in heterotopic ossification，but those in the normal bone tissue showed low express or no express. Image analysis demonstrated that the average absorbance value and the percentage of positive area of COX-2, BMP-2 and VEGF in heterotopic ossification were significantly higher than those in the normal bone tissue (P < 0.01). The percentage of positive area of COX-2 expression was closely correlated with that in the BMP-2 and VEGF in heterotopic ossification (P < 0.01). These findings suggest that COX-2, BMP-2 and VEGF may play important roles in the process of heterotopic ossification formation. COX-2 may induce the expression of BMP-2 and VEGF to promote osteogenesis and angiogenesis in the heterotopic ossification organization.

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