中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (2): 190-195.doi: 10.3969/j.issn.2095-4344.1506

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

明胶海绵/β-磷酸三钙复合物联合不同剂量碱性成纤维细胞生长因子诱导骨再生的评价

崔婷婷1，邱泽文2，贵 林1，仲维剑1，马国武1   

  1. 1大连医科大学口腔医学院附属口腔医院，辽宁省大连市 116023；2大连医科大学实验动物中心，辽宁省大连市 116044
  • 收稿日期:2018-08-17 出版日期:2019-01-18 发布日期:2019-01-18
  • 通讯作者: 仲维剑，副教授，硕士生导师，大连医科大学口腔医学院附属口腔医院，辽宁省大连市 116023
  • 作者简介:崔婷婷，女，1993年生，辽宁省铁岭市人，汉族，大连医科大学在读硕士，主要从事种植牙和骨再生技术的研究。
  • 基金资助:

    辽宁省自然科学基金(2015010313-301，项目负责人：仲维剑)

Effect of gelatin sponge/beta-tricalcium phosphate composite with different doses of basic fibroblast growth factor on bone regeneration

Cui Tingting1, Qiu Zewen2, Gui Lin1, Zhong Weijian1, Ma Guowu1   

  1. 1Hospital of Stomatology, Stomatology College of Dalian Medical University, Dalian 116023, Liaoning Province, China; 2Animal Laboratory Center, Dalian Medical University, Dalian 116044, Liaoning Province, China
  • Received:2018-08-17 Online:2019-01-18 Published:2019-01-18
  • Contact: Zhong Weijian, Associate professor, Master’s supervisor, Hospital of Stomatology, Stomatology College of Dalian Medical University, Dalian 116023, Liaoning Province, China
  • About author:Cui Tingting, Master candidate, Hospital of Stomatology, Stomatology College of Dalian Medical University, Dalian 116023, Liaoning Province, China
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No. 2015010313-301 (to ZWJ)

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摘要：

文章快速阅读：

 

文题释义：
β-磷酸三钙：主要是由钙和磷组成，其成分与骨基质的无机成分相似，能为新骨生成提供钙和磷。动物或人体细胞可在β-磷酸三钙材料上正常生长、分化和繁殖。大量实验证明，β-磷酸三钙对骨髓造血功能无不良反应，无排异反应，无急性毒性反应，不致癌变，无过敏现象，且吸收率适中，能为新骨生成提供良好的支架作用。
碱性成纤维细胞生长因子：是含155个氨基酸的促有丝分裂阳离子多肽，其中一半以上的氨基酸顺序与酸性成纤维细胞生长因子相同，相对分子质量为16 000-18 500，分子结构中有4个半胱氨基酸，以此形成分子的三维空间结构。碱性成纤维细胞生长因子通过与靶细胞上的受体结合而发生作用，因此细胞内合成的碱性成纤维细胞生长因子需分泌至细胞外才能发挥生物学作用。碱性成纤维细胞生长因子的mRNA翻译产物缺少引导它们向细胞外分泌的信号序列，其分泌途径与经典途径不同，除了可能是细胞受损或死亡后释出，还有自分泌和旁分泌起作用。碱性成纤维细胞生长因子参与招募骨祖细胞和成骨细胞，并刺激其增殖和分化，具有骨诱导作用，能促进新骨生成。
 
 
背景：目前，常用的提高组织再生率的方法是将生长因子与支架材料相结合，因此基因增强的骨组织工程方法越来越受到重视。
目的：评价明胶海绵/β-磷酸三钙复合物联合不同剂量碱性成纤维细胞生长因子的骨再生效果。
方法：取40只雄性SD大鼠，制备颅骨缺损模型，随机分4组干预，于骨缺损处分别植入单纯明胶海绵/β-磷酸三钙复合物、载10，100，200 ng碱性成纤维细胞生长因子的明胶海绵/β-磷酸三钙复合物。植入后8周取植骨材料及其下的颅骨，通过大体和组织学观察分析成骨情况。

结果与结论：各组明胶海绵/β-磷酸三钙复合物被大量的纤维结缔组织包裹，纤维结缔组织中可见大量的新生血管及淋巴细胞，在β-磷酸三钙颗粒周围可见巨噬细胞，β-磷酸三钙颗粒未见明显吸收，β-磷酸三钙与颅骨间有新骨形成，新骨骨小梁之间有较多活跃的骨细胞，编织骨结构紊乱，编织骨下方为成熟的板层骨，是未破坏的原有颅骨；载100 ng碱性成纤维细胞生长因子的明胶海绵/β-磷酸三钙复合物组新骨形成量最多，单纯明胶海绵/β-磷酸三钙复合物组新骨形成量最少。结果表明，碱性成纤维细胞生长因子联合明胶海绵/β-磷酸三钙复合物可促进骨缺损中的骨再生，其中以100 ng碱性成纤维细胞生长因子促进骨再生效果最好。

ORCID: 0000-0002-1581-1573(崔婷婷) 

关键词: 骨再生, bFGF, 碱性成纤维细胞生长因子, 明胶海绵/β-磷酸三钙复合物, 组织工程, 动物实验, 生物材料

Abstract:

BACKGROUND: The current method of improving tissue regeneration is to combine growth factors with scaffold materials. Therefore, more and more attention has been paid to the gene-enhanced tissue engineering method.

OBJECTIVE: To evaluate the effect of gelatin sponge/β-tricalcium phosphate composite mixed with different doses of basic fibroblast growth factor on bone regeneration.
METHODS: Forty male Sprague-Dawley rats were selected to prepare skull defect models. Rat models were randomly divided into four groups, and implanted with gelatin sponge/β-tricalcium phosphate composite, gelatin sponge carrying 10, 100 and 200 ng of basic fibroblast growth factor/β-tricalcium phosphate composite. After 8 weeks of implantation, bone formation was observed by gross and histological observations.
RESULTS AND CONCLUSION: In each group, the gelatin sponge/β-tricalcium phosphate composite was encapsulated by a large number of fibrous connective tissues, a large number of neovascularization and lymphocytes distributed in the fibrous connective tissue, and macrophages were visible around β-tricalcium phosphate granules. β-tricalcium phosphate granules were not obviously absorbed, and there was new bone formation between β-tricalcium phosphate and the skull. Many active bone cells existed among the new bone trabeculae, with disordered woven bone structure, the mature lamellar bone underneath the woven bone, and the undamaged original skull. The most active new bone formation was found in the basic fibroblast growth factor 100 ng+gelatin sponge/β-tricalcium phosphate composite group, and the new bone volume was the least in the solely used gelatin sponge/β-tricalcium phosphate composite. In conclusion, the combination of basic fibroblast growth factor with gelatin sponge/β-tricalcium phosphate composite can promote bone regeneration in the bone defect, and the best effect on bone regeneration is observed when 100 ng basic fibroblast growth factor is used.  

Key words: Fibroblast Growth Factor 2, Calcium Phosphates, Bone Regeneration, Tissue Engineering

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