中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (18): 3345-3348.doi: 10.3969/j.issn.1673-8225.2012.18.026

• 移植与基因 transplantation and gene • 上一篇    下一篇

吉林汉族人群HLA-A高分辨基因的多态性分析*

焦立新，杨  帆，韩  瑜，林乾飞，陈  琳   

  1. 长春市中心血站，吉林省长春市  130031
  • 收稿日期:2011-09-23 修回日期:2012-03-07 出版日期:2012-04-29 发布日期:2012-04-29
  • 通讯作者: 陈琳，长春市中心血站，吉林省长春市 130031 chenlin640713@163.com
  • 作者简介:焦立新，女，1969年生，吉林省长春市人，汉族，1992年吉林医学院毕业，主任医师，主要从事免疫血液学的研究。 lixinjiao25@163.com
  • 基金资助:

    长春市科技局资助项目(2003015)。

Human leukocyte antigen-A gene polymorphism detected by high resolution sequence based typing in Jilin Han populations  

Jiao Li-xin, Yang Fan, Han Yu, Lin Qian-fei, Chen Lin   

  1. Changchun Blood Center, Changchun  130031, Jilin Province, China
  • Received:2011-09-23 Revised:2012-03-07 Online:2012-04-29 Published:2012-04-29
  • Contact: Chen Lin, Changchun Blood Center, Changchun 130031, Jilin Province, China chenlin640713@163.com
  • About author:Jiao Li-xin, Chief physician, Changchun Blood Center, Changchun 130031, Changchun Province, China lixinjiao25@163.com
  • Supported by:

    Fund of Technology Bureau of Changchun No.2003015*

PDF

341

被引次数

7

摘要：

背景：HLA与免疫遗传学、免疫生物学密切相关，其分型对于器官移植和非感染性疾病的易感性有重要意义。
目的：调查吉林汉族人群HLA-A位点基因多态性，分析不同人群HLA-A等位基因频率分布特征。
方法：采用基因测序技术检测2 196名吉林汉族人HLA-A位点第2、3、4外显子序列，软件分析得到分型结果，计算各等位基因频率并与不同人群进行比较。
结果与结论：共检测到42种HLA-A等位基因，其中3种等位基因频率≥5%，分别是HLA-A*02:01(8.5%)，HLA-A*11:01(8.2%)和HLA-A*24:02(7.3%)，这3种等位基因频率合计为24.0%。同时，检测到39种等位基因频率<0.5%的HLA-A等位基因，这39种等位基因频率合计为76.0%。吉林汉族人群HLA-A等位基因频率分布与美国亚裔人群、中国香港华人、日本人相比存在一定差异。提示吉林汉族人群HLA-A基因的分布有地域特征。
 

关键词: 人类白细胞抗原A基因, 吉林, 汉族人群, 等位基因频率, 表现频率

Abstract:

BACKGROUND: Human leukocyte antigen (HLA) is closely related with immunogenetics and immunobiology, its types influence the outcome of clinical transplantation and confer susceptibility to a wide range of non-infectious diseases.
OBJECTIVE: To investigate the genetic polymorphism of HLA-A locus gene in Jilin Han populations and to compare the distribution characteristic of the allele frequency with that in other populations.
METHODS: A total of 2 196 blood samples from Jilin Han populations were analyzed by sequence based typing. Then the sequences encompassing exons 2, 3 and 4 for HLA-A gene were analyzed by direct sequencing of polymerase chain reaction products. The allele frequency distribution of HLA-A in this populations was compared with that in other populations.
RESULTS AND CONCLUSION: A total of 42 different HLA-A alleles were detected, and among them, 3 HLA-A alleles with frequencies were higher than 5%, they were HLA-A*02:01(8.5%), HLA-A*11:01(8.2%) and HLA-A*24:02(7.3%). The total frequency of the 3 alleles was 24.0%. At the same time, there were 39 kinds of HLA-A allele with frequencies lower than 0.5%; the total frequency of these alleles was 76.0%. There were some differences of frequency distribution of HLA-A allele in Jilin Han population when compared with American Asian, the Chinese populations from Hong Kong and Japanese, respectively. The results show the geographical characteristic of HLA-A distribution in Jilin Han populations. 

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