中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2793-2796.doi: 10.3969/j.issn.1673-8225.2012.15.029

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

两种方法培养人早孕绒毛滋养层细胞的比较***★

张小红，王会玲，白江涛，李玉红，许  倩   

  1. 承德医学院，河北省承德市  067000
  • 收稿日期:2011-10-28 修回日期:2012-03-02 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 李玉红，博士，教授，承德医学院，河北省承德市 067000 youngcheer2003@yahoo.com.cn
  • 作者简介:张小红★，女，1975年生，河北省承德市人，满族，2009年华北煤炭医学院毕业，硕士，实验师，主要从事滋养细胞肿瘤恶性侵袭的分子机制的研究。308697922@qq.com
  • 基金资助:

    2009年河北省教育厅科学研究计划项目(2009303)；2008年河北省教育厅自然科学研究计划项目(2008303)；2009年承德医学院科学研究计划项目(200919)。

Comparison of two ways to culture human primary first-trimester trophoblast cells 

Zhang Xiao-hong, Wang Hui-ling, Bai Jiang-tao, Li Yu-hong, Xu Qian   

  1. Chengde Medical University, Chengde  067000, Hebei Province, China
  • Received:2011-10-28 Revised:2012-03-02 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Li Yu-hong, Doctor, Professor, Chengde Medical University, Chengde 067000, Hebei Province, China youngcheer2003@yahoo.com.cn
  • About author:Zhang Xiao-hong, Master, Experimentalist, Chengde Medical University, Chengde 067000, Hebei Province, China 308697922@qq.com
  • Supported by:

    the Science Research Project of Hebei Educational Bureau in 2009, No. 2009303*; the Natural Science Program of Hebei Educational Bureau in 2008, No. 2008303*; the Science Research Project of Chengde Medical University in 2009, No. 200919*

PDF

298

被引次数

4

摘要：

背景：人早孕绒毛滋养层细胞体外培养是研究各种妊娠疾病的基础，如何获得纯度高、数量多的滋养层细胞以及如何简化实验步骤，仍是目前研究的热点。
目的：寻求一种培养高纯度人早孕绒毛滋养层细胞方法。
方法：取5~10周正常绒毛组织，胰酶消化和差速贴壁联合应用法与组织块培养法分别进行人早孕绒毛滋养层细胞原代培养，免疫组织化学观察细胞角蛋白7和波形蛋白的表达，进行滋养层细胞纯度的鉴定。
结果与结论：两种方法均能培养出人早孕绒毛滋养层细胞，呈三角形，多边形。抗-细胞角蛋白7阳性表达，抗-波形蛋白阴性表达。胰酶消化和差速贴壁联合应用法培养细胞纯度高于组织块培养法(P < 0.05)。提示胰酶消化和差速贴壁联合应用法是一种培养人早孕绒毛滋养层细胞较好的方法。
 

关键词: 滋养层细胞, 胰酶消化, 差速贴壁联合法, 组织块培养法, 纯度

Abstract:

BACKGROUND: In vitro culture of human primary first-trimester trophoblast cells is the study basis for various diseases in pregnancy. Currently, it is a hotspot to obtain a large amount of high-purity trophoblast cells as well as to simplify the experimental procedure.
OBJECTIVE: To explore a culture method to harvest high-purity human primary first-trimester trophoblast cells.
METHODS: Normal chorionic villi (5-10 weeks) were obtained for primary culture of human primary first-trimester trophoblast cells using tissue-culture method and trypsin digestion combined with differential adhesion method. We observed the expression of cytokeratin and vimentin by immunohistochemistry to identify the purity of cultured trophoblast cells.
RESULTS AND CONCLUSION: These two methods could obtain human primary first-trimester trophoblast cells in triangular and polygonal shape. The positive expression of cytokeratin and negative expression of vimentin were found by immunohistochemistry. The purity of cultured cells using trypsin digestion combined with differential adhesion method was higher than that using tissue-culture method (P < 0.05). Trypsin digestion combined with differential adhesion method is a better method to culture human primary first-trimester trophoblast cells.
Zhang XH, Wang HL, Bai JT, Li YH, Xu Q. Comparison of two ways to culture human primary first-trimester trophoblast cells. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2793-2796.     [http://www.crter.cn  http://en.zglckf.com]

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