中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (8): 1336-1340.doi: 10.3969/j.issn.1673-8225.2012.08.002

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

生物活性玻璃对成骨细胞Ⅰ型胶原和骨钙素基因表达的影响★

杨玉生   

  1. 南京医科大学附属无锡市第二医院骨科，江苏省无锡市  214002
  • 收稿日期:2011-06-23 修回日期:2011-09-01 出版日期:2012-02-19 发布日期:2012-02-19
  • 作者简介: 杨玉生★，男，1966年生，江苏省泰兴市人，汉族，1994年苏州大学毕业，硕士，主任医师，主要从事关节外科与组织工程方面的研究。 yangyusheng66@163.com

Effect of bioactive glass on gene expression of typeⅠcollagen and osteocalcin in osteoblasts

Yang Yu-sheng   

  1. Department of Orthopedics, Wuxi Second Hospital of Nanjing Medical University, Wuxi  214002, Jiangsu Province, China
  • Received:2011-06-23 Revised:2011-09-01 Online:2012-02-19 Published:2012-02-19
  • About author:Yang Yu-sheng★, Master, Chief physician, Department of Orthopedics, Wuxi Second Hospital of Nanjing Medical University, Wuxi 214002, Jiangsu Province, China yangyusheng66@ 163.com

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摘要：

背景：在成骨细胞培养液中加入生物活性玻璃离子，对成骨细胞的细胞因子分泌是正相关还是负相关？
目的：观察生物活性玻璃对成骨细胞Ⅰ型胶原和骨钙素基因表达的影响，以进一步探讨生物活性玻璃促进成骨细胞增殖的可能机制。
方法：以DMEM完全培养液作为对照组培养液，以加入生物活性玻璃离子液的DMEM完全培养液作为实验组培养液，取第2代乳鼠颅骨源性成骨细胞，用两种培养液分别培养4 d进行实验观察。
结果与结论：鼠成骨细胞2种生长因子的表达量实验组与对照组之比为：Ⅰ型胶原=3.376、骨钙素=2.687，两者与内参基因相对量两两比较差异有显著性意义(P < 0.05)。生物活性玻璃促进成骨细胞对Ⅰ型胶原、骨钙素的基因表达，有利于骨修复过程中成骨细胞周围基质的形成和矿化，从而促进骨愈合。
关键词：生物活性玻璃；成骨细胞；细胞因子；Ⅰ型胶原；骨钙素
doi:10.3969/j.issn.1673-8225.2012.08.002

关键词: 生物活性玻璃, 成骨细胞, 细胞因子, Ⅰ型胶原, 骨钙素

Abstract:

BACKGROUND: Is it positively or negatively correlated with osteoblastic cytokine secretion by adding bioactive glass ion to osteoblast culture fluid?
OBJECTIVE: To observe the gene expression of type Ⅰ collagen and bone gla-protein (BGP) of osteoblasts after addition with bioactive glass in order to further investigate the possible mechanisms of bioactive glass promoting osteoblast proliferation.
METHODS: The full Dulbecco’s modified eagle medium (DMEM) was prepared as the control group culture fluid, and the full DMEM culture with ionic products of bioactive glass dissolution was prepared to be the experimental group culture fluid. The second filial generation osteoblastes isolated from mouse cranial bone were cultured by two kinds of culture fluid respectively for  4 days and then, an experimental observation was done.
RESULTS AND CONCLUSION: The ratio of experimental group to control group in the gene expression quantity of the two mice osteoblast cell factors was as follows: type Ⅰ collagen=3.376 and BGP=2.687. There was significant difference in the relative amount to the inner reference gene between experimental group and control group (P < 0.05). Bioactive glass promotes the gene expression of type Ⅰ collagen and BGP in osteoblasts, which is beneficial to matrix formation and mineralization around the osteoblasts during bone repairing process, and thus promotes the bone healing.

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