中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1161-1164.doi: 10.3969/j.issn.1673-8225.2012.07.006

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

双醋瑞因对白细胞介素1β诱导软骨细胞凋亡的影响*★

廖俊琳，王  声，刘日光   

  1. 贵阳医学院附属医院骨科，贵州省贵阳市 550001
  • 收稿日期:2011-09-28 修回日期:2011-12-25 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 刘日光，博士，教授，主任医师，硕士生导师，贵阳医学院附属医院骨科，贵州省贵阳市 550001 rgliu@vip.sina.com
  • 作者简介:廖俊琳★，男，1985年生，湖南省娄底市人，汉族，贵阳医学院在读硕士，主要从事骨关节病的基础研究。liaojunlin85@163.com
  • 基金资助:

    贵州省自然科学基金资助项目：黔科合J字[2008]2286号。

Effects of Diacerein on apoptosis of chondroctyes induced by interleukin-1 beta 

Liao Jun-lin, Wang Sheng, Liu Ri-guang   

  1. Department of Orthopedics, Hospital of Guiyang Medical University, Guiyang  550001, Guizhou Province, China
  • Received:2011-09-28 Revised:2011-12-25 Online:2012-02-12 Published:2012-02-12
  • Contact: Liu Ri-guang, Doctor, Professor, Chief physician, Master’s supervisor, Department of Orthopedics, Hospital of Guiyang Medical University, Guiyang 550001, Guizhou Province, China rgliu@sina.vip.com
  • About author:Liao Jun-lin★, Studying for master’s degree, Department of Orthopedics, Hospital of Guiyang Medical University, Guiyang 550001, Guizhou Province, China liaojunlin85@163.com
  • Supported by:

    the Natural Science Foundation of Guizhou Province, No. [2008]2286*

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摘要：

背景：双醋瑞因是一种新型的白细胞介素1阻滞剂，其是否对软骨细胞的凋亡有抑制作用以及是否通过细胞信号转导通路发挥作用的基础应用研究较少。
目的：观察双醋瑞因对体外白细胞介素1β诱导大鼠关节软骨细胞凋亡的影响。
方法：体外培养SD大鼠关节软骨细胞，苏木精-伊红染色和Ⅱ型胶原免疫荧光染色鉴定软骨细胞，用白细胞介素1β诱导大鼠关节软骨细胞凋亡模型，再用10-4，10-5，10-6 mol/L双醋瑞因干预培养。
结果与结论：10-4 mol/L和10-5 mol/L的双醋瑞因可降低白细胞介素1β诱导的软骨细胞凋亡率(P < 0.01和P < 0.05)。      10-5 mol/L双醋瑞因能够显著抑制白细胞介素1β诱导p38磷酸化(P < 0.01)。双醋瑞因干预的软骨细胞中p38 mRNA的表达量较模型组下降0.38倍(P < 0.01)。结果证实，双醋瑞因能够抑制软骨细胞中p38蛋白和基因的表达，从而抑制白细胞介素1β诱导关节软骨细胞凋亡。

关键词: 双醋瑞因, 骨性关节炎, 软骨细胞, 白细胞介素1&beta, 细胞凋亡vp38丝裂原活化蛋白激酶, 骨组织工程

Abstract:

BACKGROUND: Diacerein is a novel interleukin-1 blockers, researches regarding whether it can inhibit the apoptosis of chondrocytes and whether it plays the role through signaling pathway or not are rately.
OBJECTIVE: To investigate the effects of Diacerein on the apoptosis of chondroctyes induced by interleukin-1 beta (IL-1β).
METHODS: Articular chondrocytes of Sprague Dawley rats were cultured in vitro and identified by hematoxylin-eosin staining and immunofluorescence staining of collagen typeⅡ. The rats apoptosis model of articular chondrocytes was induced by IL-1β, and then treated with different concentrations of diacerein (10-4, 10-5, 10-6 mol/L).
RESULTS AND CONCLUSION: Diacerein with the concentrations of 10-4 mol/L and 10-5 mol/L could decrease the apoptotic rate of chondrocytes which induced by IL-1β (P < 0.01, P < 0.05). Diacerein with the concentrations of 10-5 mol/L could inhibit phosphorylation-p38 which induced by IL-1β (P < 0.01). Compared with the control group, the expression of p38 mRNA of model group was decreased by 3.78 times (P < 0.01). Diacerein can inhibit IL-1β-induced early apoptosis. The possible mechanism is related to the effects of Diacerein on the inhibition of the expression the protein and gene of p38.

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