中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (2): 261-264.doi: 10.3969/j.issn.1673-8225.2012.02.016

• 骨组织构建 • 上一篇    下一篇

“双固一通”温针灸实验性膝骨性关节炎模型兔Bcl-2及Bax蛋白的表达

熊  勇，彭  锐，王  华
  

  1. 湖北中医药大学针灸骨伤学院，湖北省武汉市  430061
  • 收稿日期:2011-07-10 修回日期:2011-09-26 出版日期:2012-01-08 发布日期:2012-01-08
  • 通讯作者: 王华，教授，博士生导师，湖北中医药大学针灸骨伤学院，湖北省武汉市 430061 Hwang.tcm@china.com
  • 作者简介:熊勇☆，男，1978年生，湖北省武汉市人，汉族，2010年湖北中医药大学毕业，博士，讲师，主要从事老年性骨关节病的研究。 xiongyong1978@163.com
  • 基金资助:

    湖北省中医药中西医结合科研课题(2008Z-Z01)。

Effects of “Shuanggu Yitong” warming acupuncture on the expressions of Bcl-2 and Baxproteins in the cartilage of knee osteoarthritis rabbits

Xiong Yong, Peng Rui, Wang Hua   

  1. College ofAcupuncturemoxibustionandOrthopedics, HubeiUniversity of ChineseTraditional Medicine,Wuhan 430061,Hubei Province,China
  • Received:2011-07-10 Revised:2011-09-26 Online:2012-01-08 Published:2012-01-08
  • Contact: Wang Hua,Professor, Doctoralsupervisor, College ofAcupuncturemoxibustionandOrthopedics, HubeiUniversity of ChineseTraditional Medicine,Wuhan 430061,Hubei Province,China Hwang.tcm@china.com
  • About author: Xiong Yong☆,Doctor, Lecturer,College ofAcupuncturemoxibustionandOrthopedics, HubeiUniversity of ChineseTraditional Medicine,Wuhan 430061,Hubei Province,China xiongyong1978@163.com
  • Supported by:

    HubeiProvincial ScientificResearch Foundationof Chinese TraditionalMedicine for theCombination ofChinese Traditionaland WesternMedicine, No.2008Z-Z01*

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摘要：

背景：软骨细胞凋亡在骨性关节炎的发生发展中起到关键作用。
目的：观察“双固一通”温针灸预防兔膝骨性关节炎过程中对兔膝关节软骨Bcl-2、Bax蛋白表达的影响。
方法：采用结扎股静脉法建立兔膝骨性关节炎模型，造模后采用“双固一通”温针灸法进行干预，针灸穴位包括关元(CV 4)、足三里(ST 36)、内膝眼(EX-LE4)、犊鼻(ST 35)和阳陵泉(GB 34)，1次/d，留针20 min，连续8周。
结果与结论：膝骨性关节炎后，兔的软骨细胞凋亡指数明显升高(P < 0.01)，“双固一通”温针灸可降低软骨细胞凋亡指数(P < 0.01)。免疫组织化学染色显示：“双固一通”温针灸兔和模型兔关节软骨Bcl-2表达明显增高(P < 0.05)，而“双固一通”温针灸兔和正常兔关节软骨Bax的表达明显低于模型兔(P < 0.05)，“双固一通”温针灸兔Bcl-2/Bax高于模型兔和正常兔(P < 0.05)。说明“双固一通”温针灸可上调Bcl-2蛋白表达，下调Bax蛋白表达抑制软骨细胞过度凋亡，对兔膝骨性关节炎起防治作用。

关键词: &ldquo, 双固一通&rdquo, 温针灸, 骨性关节炎, 凋亡, Bcl-2, Bax

Abstract:

BACKGROUND: Apoptosis of chondrocyte plays an vital role in the initiation and development of osteoarthritis.
OBJECTIVE: To explore the effects of “Shuanggu Yitong” (double-reinforcing and one-unblocking) warming acupuncture on theexpressions of Bcl-2 and Bax proteins in cartilage of knee osteoarthritis rabbits.
METHODS: Knee osteoarthritis model was established by the ligation of femoral vein. Animals were treated with “ShuangguYitong” warming acupuncture applied on Guanyuan (CV 4), Zusanli (ST 36), Neixiyan (EX-LE4), Dubi (ST 35) and Yanglingquan(GB 34) after model establishment. Needle was retained for 20 minutes on each acupoint daily for 8 weeks.
RESULTS AND CONCLUSION: Rabbit apoptosis index of chondrocyte was significantly increased after knee osteoarthritis(P < 0.01). Apoptosis index of chondrocyte was reduced by “Shuanggu Yitong” warming acupuncture. According toimmunohistochemical staining, the expression of Bcl-2 in articular cartilage was significantly increased in the “Shuanggu Yitong”warming acupuncture group and model group (P < 0.05). While the Bax expression in articular cartilage of “Shuanggu Yitong”warming acupuncture group and control group was significantly lower than that of model group (P < 0.05). The expression ofBcl-2 and Bax of “Shuanggu Yitong” warming acupuncture group and model group was higher than that of the model group andcontrol group (P < 0.05). These findings demonstrate that “Shuanggu Yitong” warming acupuncture can up-regulate theexpression of Bcl-2 protein as well as down-regulate expression of Bax protein, thereby inhibit the apoptosis of cartilage cells,which may prevent and cure knee osteoarthritis in rabbits.

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