中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (51): 9633-9636.doi: 10.3969/j.issn.1673-8225.2011.51.031

• 材料力学及表面改性 material mechanics and surface modification • 上一篇    下一篇

胆甾醇疏水改性的羧乙基普鲁兰多糖合成及自组装性质

陶晓军1，唐红波1，凌  凯1，王  艳1，张其清1,2   

  1. 1北京协和医学院，中国医学科学院生物医学工程研究所，天津市生物医学材料重点实验室，天津市  300192
    2厦门大学生物医学工程研究中心厦门大学医学院，厦门市生物医学工程技术研究中心，福建省厦门市  361005
  • 收稿日期:2011-04-11 修回日期:2011-09-12 出版日期:2011-12-17 发布日期:2011-12-17
  • 通讯作者: 张其清，博士，教授，博士生导师，中国医学科学院生物医学工程研究所，天津市生物材料重点实验室，天津市 300192；厦门大学生物医学工程研究中心，福建省厦门市 361005 zhangqiq@126.com
  • 作者简介:陶晓军☆，男，1981 年生，湖北省麻城市人，汉族，2011年北京协和医学院毕业，博士，主要从事多糖疏水改性及其纳米粒子与生物大分子相互作用研究。 taoxiaojun428@sohu.com
  • 基金资助:

    国家重大科学研究计划项目(2006CB933300)；博士点基金(20091106120051)。

Synthesis of cholesterol-modified pullulan and its self-assembled behavior

Tao Xiao-jun1, Tang Hong-bo1, Ling Kai1, Wang Yan1, Zhang Qi-qing1, 2   

  1. 1Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin  300192, China
    2Research Center of Biomedical Engineering, Xiamen University, Xiamen  361005, Fujian Province, China
  • Received:2011-04-11 Revised:2011-09-12 Online:2011-12-17 Published:2011-12-17
  • Contact: Zhang Qi-qing, Doctor, Professor, Doctoral supervisor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China; Research Center of Biomedical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China zhangqiq@126.com
  • About author:Tao Xiao-jun☆, Doctor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China taoxiaojun428@ sohu.com
  • Supported by:

    the National Major Science Research Plan of China, No. 2006CB933300*; the Doctoral Foundation, No. 20091106120051*

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摘要：

背景：将羧基引入疏水改性的多糖，使其自组装的纳米粒子带上表面电荷，有利于提高其稳定性，更好的发挥作为药物载体的优良性质。
目的：观察合成胆甾醇疏水改性的羧乙基普鲁兰多糖在水溶液中的自组装行为及其纳米粒子的性质。
方法：利用丙烯酸与普鲁兰多糖反应生成羧乙基普鲁兰多糖(CEP)，然后按前期方法合成胆甾醇琥珀酰基N-羟基琥珀酰亚胺(CSN)，最后CSN和CEP在催化剂下生成目标物。应用核磁共振仪对产物进行表征。
结果与结论：通过1H-NMR分析，表明成功合成了胆甾醇疏水改性的羧乙基普鲁兰多糖，该材料在水溶液中能自组装为具有乳光的球型纳米粒子。CHS-CEP纳米粒子平均粒径为148.6 nm，平均Zeta电位约为-19.9 mV，在水溶液中分散度好，分布均匀，具有长期稳定性，可作为良好的药物载体。

关键词: 透射电镜, 动态光散射分析仪, 药物载体, 羧乙基普鲁兰多糖, 胆甾醇, 纳米粒子

Abstract:

BACKGROUND: Carboxyl group is introduced to hydrophobically modify polysaccharides, which could self-aggregate to nanoparticles with some surface charges, and it is beneficial to improving their stability and performing the good properties as a drug carrier.
OBJECTIVE: To synthesis cholesterol hydrophobically modified carboxyethyl pullulan (CHS-CEP), and to study their self-aggregated behavior in aqueous solution and characteristics of CHS-CEP nanoparticles.
METHODS: Acrylic acid and pullulan polysaccharides could form carboxyethyl pullulan (CEP) by additive reaction. Objective material could be obtained by catalyzing CEP and CSN. The synthesized material was characterized using proton magnetic resonance (1H-NMR). The nanoparticles were prepared by dialysis to characterize the morphology, size and zeta potential by transmission electron microscopy (TEM) and dynamic laser scattering (DLS).
RESULTS AND COUCLUSION: By 1H-NMR analysis, it showed that CHS-CEP was successfully synthesized and this material could self-aggregate to form nanoparticles with opalescence. The diameter of CHS-CEP nanoparticles was about 148.6 nm and their zeta potential was -19.9 mV. They could act as drug carrier due to many excellent properties such as a good dispersity in aqueous solution, even distribution and long-term stability.

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