中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8595-8597.doi: 10.3969/j.issn.1673-8225.2011.46.011

• 骨组织构建 bone tissue construction • 上一篇    下一篇

金乌骨通胶囊对成骨细胞分泌白细胞介素1，6的影响

刘春颖1，郑文奎2，祖金池2，周玉娟1，刘  莉1，王恩军1，李少春1   

  1. 1河北大学医学部药理教研室，河北省保定市 071000
    2河北大学附属医院，河北省保定市 071000
  • 收稿日期:2011-06-25 修回日期:2011-07-04 出版日期:2011-11-12 发布日期:2011-11-12
  • 作者简介:刘春颖★，女，1977年生，河北省保定市人，硕士，讲师，主要从事骨质疏松药理学方面的研究。 liuchy310@163.com
  • 基金资助:

    河北省中医药管理局科研计划项目(No.2009149)，金乌骨通胶囊含药血清对成骨细胞骨保护素/核因子κB受体活化因子配体分泌的影响。

Effects of Jinwugutong capsule on the expression of interleukin1, 6 in osteoblasts

Liu Chun-ying1, Zheng Wen-kui2, Zu Jin-chi2, Zhou Yu-juan1, Liu Li1, Wang En-jun1, Li Shao-chun1   

  1. 1Department of Pharmacology, Hebei University Health Science Center, Baoding 071000, Hebei Province, China
    2 Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China
  • Received:2011-06-25 Revised:2011-07-04 Online:2011-11-12 Published:2011-11-12
  • About author:Liu Chun-ying★, Master, Lecturer, Department of Pharmacology, Hebei University Health Science Center, Baoding 071000, Hebei Province, China liuchy310@163.com
  • Supported by:

    Scientific Research Development Project of Administration of Traditional Chinese Medicine of Hebei Province, No. 2009149*

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摘要：

背景：课题组以往研究发现，金乌骨通胶囊对绝经后骨质疏松症具有明显的治疗作用，但其具体的机制尚不十分清楚。
目的：观察金乌骨通胶囊含药血清对成骨细胞分泌白细胞介素1，6的影响。
方法：切除雌性SD大鼠双侧卵巢制备去卵巢血清，灌胃给予去卵巢大鼠金乌骨通胶囊制备去卵巢含药血清。取第3代生长状况良好的出生24 h内SD大鼠的成骨细胞，分别加入正常雌性SD大鼠血清、去卵巢血清和去卵巢含药血清培养72 h。
结果与结论：酶联免疫吸附法检测显示，与正常血清组比较，去卵巢血清组成骨细胞白细胞介素1、白细胞介素6的表达明显升高(P < 0.01)；与去卵巢血清组比较，去卵巢含药血清组白细胞介素1和白细胞介素6的表达明显降低(P < 0.05)，与正常血清组比较，则无明显差异。提示金乌骨通胶囊可能通过抑制成骨细胞骨白细胞介素1和白细胞介素6的表达来实现治防治骨质疏松症的目的。

关键词: 金乌骨通胶囊, 含药血清, 成骨细胞, 白细胞介素1, 白细胞介素6

Abstract:

BACKGROUND: Our previous studies have shown that Jinwugutong capsule exhibits obvious effects on postmenopausal osteoporosis.
OBJECTIVE: To investigate the effects of Jinwugutong capsule-containing serum on the expression of interleukin (IL)-1 and IL-6 in osteoblasts.
METHODS: Bilateral ovaries of female Sprague-Dawley (SD) rats were removed to prepare ovarian serum. Ovariotomized (OVX) rats were intragastrically administered Jinwugutong capsule to prepare ovarian medicated serum. Passage 3 osteoblasts which were harvested from rats born within 24 hours were cultured with normal female rat serum (normal serum group), OVX rat serum (OVX serum group) and OVX rat Jinwugutong capsule-containing serum (OVX drug-containing serum group) for 72 hours separately.  
RESULTS AND CONCLUSION: ELISA results showed that IL-1 and IL-6 expression in the osteoblasts was significantly increased in the OVX serum group than in the normal serum group (P < 0.01); IL-1 and IL-6 expression in the osteoblasts was significantly decreased in the OVX drug-containing serum group than in the OVX serum group, but there was no significant difference in IL-1 and IL-6 expression between normal serum group and OVX drug-containing serum group. These findings suggest that Jinwugutong capsule can prevent and treat osteoporosis by inhibiting IL-1 and IL-6 expression in osteoblasts. 

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