中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (46): 8561-8564.doi: 10.3969/j.issn.1673-8225.2011.46.003

• 软骨组织构建 cartilage tissue construction • 上一篇    下一篇

射频能量处理软骨损伤的急性效应

陈  琦1，王大平2，朱伟民2，刘建全2   

  1. 1广州医学院，广东省广州市510182
    2深圳市第二人民医院创伤骨科，广东省深圳市  518035
  • 收稿日期:2011-06-15 修回日期:2011-09-15 出版日期:2011-11-12 发布日期:2011-11-12
  • 通讯作者: 王大平，博士，主任医师，硕士生导师，深圳市第二人民医院，广东省深圳市 518035 dapingwang@medmail.com
  • 作者简介:陈琦★，男，1986年生，河南省遂平县人，汉族，广州医学院在读硕士，医师，主要从事创伤骨科与骨关节镜研究。 chenqi17@163.com
  • 基金资助:

    深圳市科技计划项目(201001013)组织工程软骨治疗骨性关节炎软骨损伤的实验研究。

Acute effect of radiofrequency energy on cartilage injury

Chen Qi1, Wang Da-ping2, Zhu Wei-min2, Liu Jian-quan2   

  1. 1Guangzhou Medical University, Guangzhou  510182, Guangdong Province, China
    2Department of Orthopedic Trauma, the Second People’s Hospital of Shenzhen. Shenzhen 518035, Guangdong Province, China
  • Received:2011-06-15 Revised:2011-09-15 Online:2011-11-12 Published:2011-11-12
  • Contact: Wang Da-ping, Doctor, Chief physician, Master’s supervisor, Department of Orthopedic Trauma, the Second People’s Hospital of Shenzhen, Shenzhen 518035, Guangdong Province, China dapingwang@medmail.com
  • About author:Chen Qi★, Studying for master’s degree, Physician, Guangzhou Medical University, Guangzhou 510182, Guangdong Province, China chenqi17@163.com
  • Supported by:

    the Science and Technology Programme of Shenzhen, No. 201001013*

PDF

273

被引次数

5

摘要：

背景：射频消融能量近年来用于关节软骨损伤的处理，但临床上对其使用时能量设置的大小还存在较大争议。
目的：评估不同大小的双极射频消融能量处理Ⅱ级软骨损伤的急性效应，得出射频能量大小与疗效的关系。
方法：选取3个新鲜的牛膝关节并建立Ⅱ级关节软骨损伤模型，使用双极射频气化仪在30，50，70，90，110 W的不同能量设置下，处理关节损伤区30 s，同时设置对照组。
结果与结论：扫描电镜检测显示，经双极射频处理后，软骨表面变得平滑，在70 W的双极射频能量设置下即可使软骨表面足够光滑；葡糖胺聚糖释放率与射频能量的增加呈反向变化(P < 0.05)。结果证实随着射频能量的增加，软骨细胞活性逐渐降低，因此在达到治疗效果的目的下应尽量降低射频能量的大小。

关键词: 射频消融, 关节软骨, 软骨细胞, 急性效应, 组织工程

Abstract:

BACKGROUND: Articular cartilage injury has recently been treated with radiofrequency energy. But there still exists controversy about the energy settings of radiofrequency energy in clinical use.
OBJECTIVE: To evaluate the acute effect of bipolar radiofrequency energy with varying intensities on grade Ⅱ cartilage injury, and to explore the relationship between the intensity of radiofrequency energy and curative effect. 
METHODS: Three fresh bovine joints were selected to establish grade Ⅱ cartilage injury models. Then the joints were treated by the bipolar radiofrequency energy instrument for 30 seconds under different energy settings (30 W, 50 W, 70 W, 90 W, 110 W). At the same time, the control groups received the corresponding treatment.
RESULTS AND CONCLUSION: According to scanning electron microscope, the cartilage surface became smooth after treatment. Therapeutic effect was achieved under the radiofrequency energy setting of 70 W. There was a negative correlation between glycosaminoglycan release rate and radiofrequency energy intensity (P < 0.05). It indicates that cartilage cell activity decreased with the increasing of radiofrequency energy intensity. Therefore, the radiofrequency energy intensity should be controlled in minimum amount without compromising therapeutic efficacy.

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