中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (45): 8487-8490.doi: 10.3969/j.issn.1673-8225.2011.45.028

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

急性淋巴细胞白血病的免疫分型及CD4+CD25+T调节细胞检测

巫翠萍1，覃  西1，巫翠云2，朱  洪1，周海燕1   

  1. 1海南医学院附属医院，海南省海口市  570102
    2海口市人民医院，海南省海口市570208
  • 收稿日期:2011-08-10 修回日期:2011-09-07 出版日期:2011-11-05 发布日期:2011-11-05
  • 通讯作者: 覃西，教授，海南医学院附属医院，海南省海口市 570102 qinxi99@21cn.com
  • 作者简介:巫翠萍，女，1962年生，海南省海口市人，汉族，2002年海南医学院毕业，副主任检验师，主要从事临床血液病检验及与流式细胞术的应用与研究。 wucuiping2007@163.com
  • 基金资助:

    海南省卫生厅科研立项课题项目(琼卫2008-55)。

Immunophenotype of acute lymphoblastic leukemia and detection of CD4+CD25+ regulatory T cells

Wu Cui-ping1, Qin Xi1, Wu Cui-yun2, Zhu Hong1, Zhou Hai-yan1   

  1. 1Affiliated Hospital of Hainan Medical College, Haikou  570102, Hainan Province, China
    2Haikou People’s Hospital, Haikou  570208, Hainan Province, China
  • Received:2011-08-10 Revised:2011-09-07 Online:2011-11-05 Published:2011-11-05
  • Contact: Qin Xi, Professor, Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China qinxi99@21cn.com
  • About author:Wu Cui-ping, Associate chief examiner, Affiliated Hospital of Hainan Medical College, Haikou 570102, Hainan Province, China wucuiping2007@163.com
  • Supported by:

    the Science and Technology Research Project of Hainan Health Bureau, No. 2008-55*

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摘要：

背景：在急性淋巴细胞白血病发病过程中，CD4+CD25+T调节细胞对机体免疫反应可能起着一定的调节作用。
目的：观察急性淋巴细胞白血病患者的免疫分型及外周血CD4+CD25+T调节细胞的变化情况。
方法：采用流式细胞仪对35例急性淋巴细胞白血病患者进行免疫分型，并检测外周血CD4+CD25+T调节细胞的数目，与18名健康对照作比较。
结果与结论：急性B细胞淋巴细胞白血病22例，急性T细胞淋巴细胞白血病13例；22例急性B细胞淋巴细胞白血病中CD19的阳性表达率最高(100%)，而13例急性T细胞淋巴细胞白血病中CD7阳性表达率最高(100%)。急性B细胞淋巴细胞白血病患者外周血CD4+CD25+T调节细胞和急性T细胞淋巴细胞白血病患者差异无显著性意义(P > 0.05)，但均高于健康对照(P < 0.05)。表明急性B细胞淋巴细胞白血病中CD19阳性表达率最高，急性T细胞淋巴细胞白血病中CD7阳性表达率最高，同时急性淋巴细胞白血病患者外周血CD4+CD25+T调节细胞水平显著增高。

关键词: 急性淋巴细胞白血病, 流式细胞仪, 免疫分型, 外周血, CD4+CD25+T调节细胞

Abstract:

BACKGROUND: CD4+CD25+ regulatory T cells (Treg) may have a certain regulatory effect on immune response in the pathogenic process of acute lymphoblastic leukemia (ALL).
OBJECTIVE: To explore the immunophenotype and the changes of Treg in the peripheral blood of patients with ALL.
METHODS: The immunophenotype and levels of CD4+CD25+Treg in the peripheral blood were detected using flow cytometry in 35 ALL patients in comparison with the health control people.
RESULTS AND CONCLUSION: There were 22 B-ALL cases and 13 T-ALL cases among 35 ALL patients. CD19 expression of the cases of B-ALL had the highest positive rate (100%); meanwhile, CD7 expression of the cases of T-ALL had the highest positive rate (100%). There were no significant difference between the number of CD4+CD25+ Treg cells in B-ALL and T-ALL patients (P > 0.05), but there were significant difference between the number of Treg cells in health control people and ALL patients (P < 0.05). The results show that CD19 expression of the cases of B-ALL and CD7 expression of the cases of T-ALL had the highest positive rate; the levels of CD4+CD25+ Treg cells significantly increased in the peripheral blood of ALL patients.

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