中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (42): 7831-7834.doi: 10.3969/j.issn.1673-8225.2011.42.010

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

胶原-纳米羟基磷灰石复合支架的细胞相容性

刘  鹏，王  东，孙海钰，刘  亮，栗树伟，贺晋栋   

  1. 山西医科大学，山西省太原市  030001
  • 收稿日期:2011-04-06 修回日期:2011-06-22 出版日期:2011-10-15 发布日期:2011-10-15
  • 通讯作者: 王东，博士生导师，教授，主任，山西医科大学第二医院创伤骨科，山西省太原市 030001 Wangd080@ sina.com
  • 作者简介:刘鹏★，男，1985年生，山西省大同市人，汉族，山西医科大学在读硕士，医师，主要从事骨折愈合机制的研究。 doubleidiot@ 163.com

Cellular compatibility of a nano-hydroxyapatite/collagen scaffold

Liu Peng, Wang Dong, Sun Hai-yu, Liu Liang, Li Shu-wei, He Jin-dong   

  1. Second Hospital Affiliated to Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Received:2011-04-06 Revised:2011-06-22 Online:2011-10-15 Published:2011-10-15
  • Contact: Wang Dong, Professor, Doctoral supervisor, Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi Province, China Wangd080@sina.com
  • About author:Liu Peng★, Studying for master’s degree, Physician, Second Hospital Affiliated to Shanxi Medical University, Taiyuan 030001, Shanxi Province, China doubleidiot@163.com

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311

被引次数

18

摘要：

背景：观察成骨细胞在生物材料上的形态、增殖和分化等项目，可评估生物支架材料的生物相容性。
目的：观察复合支架材料纳米羟基磷灰石/胶原对成骨细胞增殖、分化的影响。
方法：取新生24 h内Wistar大鼠的颅盖骨，采用改良胶原酶消化法进行成骨细胞原代培养，取第3代细胞与纳米羟基磷灰石/胶原支架或普通羟基磷灰石材料体外复合培养。培养3，6，9 d后，观察材料周边的细胞形态及支架材料对细胞分化、增殖的影响。
结果与结论：纳米羟基磷灰石/胶原材料较普通的羟基磷灰石材料更有利于成骨细胞的黏附、生长、分化、增殖，证实其生物相容性更好，有望成为一种新型的骨组织工程支架材料。

关键词: 胶原, 纳米羟基磷灰石, 复合支架, 细胞相容性, 生物材料, 成骨细胞, 组织工程支架材料, 骨组织工程

Abstract:

BACKGROUND: Under the theory of bone-tissue engineering, the biocompatibility of biomaterial scaffolds is evaluated by observing the morphology, proliferation and differentiation of osteoblasts on the biomaterials.
OBJECTIVE: To observe the effects of nano-hydroxyapatite/collagen (nHAC) scaffold on the proliferation and differentiation of osteoblasts.
METHODS: The rat osteoblasts were obtained from the cranium of newborn Wistar rats within 24 hours, and primarily cultured using modified collagenase digestion. The cells at passage 3 were co-cultured with nHAC in vitro. At 3, 6 and 9 days of the culture, cell morphology was observed by inverted phase-contrast microscopy. In addition, MTT assay and alkaline phosphatase (ALP) activity test were used to observe the effects of the material on cell differentiation and proliferation. 
RESULTS AND CONCLUSION: Osteoblasts can adhere, grow, differentiate and proliferate better on nHAC than HA. The composite scaffold nHAC has a good compatibility, indicating that the material has a great potential for application in bone tissue engineering.

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