中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (40): 7597-7600.doi: 10.3969/j.issn.1673-8225.2011.40.044

• 干细胞临床实践 clinical practice of stem cells • 上一篇    

大肠癌患者外周血细胞CD133的表达

黄颖烽1，魏亚明2，李秋娴2，聂玉强3，古维立1   

  1. 广州市第一人民医院，1普外科，2输血科，3消化内科，广东省广州市   510180
  • 收稿日期:2011-02-21 修回日期:2011-05-18 出版日期:2011-10-01 发布日期:2011-10-01
  • 通讯作者: 魏亚明，博士，研究员，主任技师，广州市第一人民医院输血科，广东省广州市 510180 weiyaming@163.com
  • 作者简介:黄颖烽★，男，1966年生，广东省翁源县人，汉族，1990年暨南大学医学院毕业，硕士，主任医师，主要从事胃肠外科肿瘤与胰腺病研究。
  • 基金资助:

    广州市广东省医学科研基金(A2008517)；广州市医药卫生科技项目(2007-YB-004)；广州市应用基础项目(2008J1-C171)资助。

CD133 expression in peripheral blood cells of patients with colorectal cancer 

Huang Ying-feng1, Wei Ya-ming2, Li Qiu-xian2, Nie Yu-qiang3, Gu Wei-li1   

  1. 1General Surgery Department, 2Blood Transfusion Department, 3Department of Gastroenterology and Hepatology, the First People’s Hospital of Guangzhou, Guangzhou  510180, Guangdong Province, China
  • Received:2011-02-21 Revised:2011-05-18 Online:2011-10-01 Published:2011-10-01
  • Contact: Wei Ya-ming, Doctor, Researcher, Chief technician, Blood Transfusion Department, the First People’s Hospital of Guangzhou, Guangzhou 510180, Guangdong Province, China weiyaming@163.com
  • About author:Huang Ying-feng★, Master, Chief physician, General Surgery Department, the First People’s Hospital of Guangzhou, Guangzhou 510180, Guangdong Province, China
  • Supported by:

    Guangdong Provincial Medical Research Foundation, No. A2008517*; Guangzhou Medical and Health Science and Technology Program, No. 2007-YB-004*; Guangzhou Applied Basic Project, No. 2008J1-C171*

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324

被引次数

4

摘要：

背景：近期有报道称在肿瘤患者外周血中检测出了高于正常值的CD133阳性细胞。
目的：观察CD133 mRNA和蛋白在大肠癌患者外周血中的表达并探讨其临床意义。
方法：应用流式细胞技术与RT-PCR反应检测29例大肠癌患者和20例正常对照外周血中CD133 抗原与mRNA的表达。
结果与结论：发生转移的大肠癌患者外周血中CD133阳性细胞比例显著高于正常对照及无转移的大肠癌患者(P < 0.01)。正常健康人群和无转移大肠癌患者外周血中CD133mRNA表达为阴性，发生转移的部分大肠癌患者外周血中CD133mRNA表达阳性，阳性率为35.7%(5/14)，在超过38个以上循环时表达为弱阳性。结果表明，无转移大肠癌患者外周血中CD133 mRNA和蛋白表达与正常对照无区别，发生转移的大肠癌患者外周血中CD133 mRNA和蛋白高于正常对照和未发生转移者。

关键词: 大肠癌, 转移, 肿瘤干细胞, CD133, RT-PCR

Abstract:

BACKGROUND: It has been reported recently that a higher CD133 positive expression in the peripheral blood of cancer patients was detected.
OBJECTIVE: To investigate the expression and clinical value of CD133 mRNA in the peripheral blood of patients with colorectal cancer.
METHODS: The flow cytometry method and RT-PCR were used to detect the expression of CD133 mRNA and protein in peripheral blood samples which were from 20 controls and 29 patients with colorectal cancer.
RESULTS AND CONCLUSION: The CD133+ cell percentages in both peripheral blood samples of controls and colorectal cancer patients without metastasis were lower than those of colorectal cancer patients with metastasis (P < 0.01). RT-PCR results revealed 5 cases of positive CD133 mRNA expression in all 14 colorectal cancer patients with metastasis, and the positive ratio was 35.7%, in which run more than 38 PCR circles. The expression of CD133 in peripheral blood cells of colorectal cancer patients with metastasis is higher than that of normal controls and colorectal cancer patients without metastasis.

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