中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (37): 6909-6912.doi: 10.3969/j.issn.1673-8225.2011.37.017

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

S100a7蛋白在体外培养人视网膜色素上皮细胞中的表达及功能

许光军，张学东，陈雪梅   

  1. 重庆医科大学附属第一医院眼科，眼科学重庆市省部级重点实验室，重庆市眼科研究所，重庆市 400016
  • 收稿日期:2011-03-09 修回日期:2011-03-24 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 张学东，教授，重庆医科大学附属第一医院眼科，眼科学重庆市省部级重点实验室，重庆市眼科研究所，重庆市 400016 zxued@sina.com
  • 作者简介:许光军★，男，1982年生，山东省乐陵市人，汉族，重庆医科大学硕士在读，主要从事眼底病研究。 308855959@qq.com

Expression identification and preliminary function analysis of S100a7 in retinal pigment epithelial cells cultured in vitro

Xu Guang-jun, Zhang Xue-dong, Chen Xue-mei   

  1. Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing 400016, China
  • Received:2011-03-09 Revised:2011-03-24 Online:2011-09-10 Published:2011-09-10
  • Contact: Zhang Xue-dong, Professor, Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing 400016, China zxued@sina.com
  • About author:Xu Guang-jun★, Studying for master’s degree, Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, Chongqing 400016, China 308855959@qq.com

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256

被引次数

2

摘要：

背景：S100a7蛋白在细胞增殖、血管形成等病理生理过程中发挥重要作用，但在视网膜色素上皮细胞中的作用少有报道。
目的：探讨S100a7蛋白在体外培养人视网膜色素上皮细胞中的表达及作用。
方法：体外培养ARPE-19细胞株，通过免疫荧光及Western blot技术，检测S100a7在ARPE-19细胞中的表达；不同稀释度(1∶1 000，1∶10 000，1∶100 000)S100a7蛋白抗体与ARPE-19细胞共培养，通过MTT技术检测S100a7蛋白在人视网膜色素上皮细胞增殖过程中的作用。
结果与结论：免疫荧光及Western blot技术证实该蛋白在ARPE-19细胞中表达；MTT实验结果显示，人视网膜色素上皮细胞加入S100a7抗体72 h后，各浓度处理组的吸光度值较对照组显著均降低(P < 0.05)。结果证实S100a7蛋白在人视网膜色素上皮细胞中的表达，并明显促进人视网膜色素上皮细胞增殖。

关键词: 人视网膜色素上皮细胞, S100a7蛋白, MTT法, 免疫荧光, Western blot

Abstract:

BACKGROUND: S100a7 is a new protein that participates in cell proliferation, vascularization and other pathophysiological processes.
OBJECTIVE: To discuss the expression of S100a7 in retinal pigment epithelial cell (RPE) and to investigate the effects of S100a7 on RPE proliferation.
METHODS: Immunofluorescence and western blot techniques were used to detect the expression of S100a7 in ARPE-19, and MTT was performed to investigate the effects of S100a7 on RPE proliferation.
RESULTS AND CONCLUSION: S100a7 was proven to be expressed in ARPE-19 by immunofluorescence and western blot techniques and it could significantly promote ARPE-19 cell proliferation. This study first confirmed the expression of S100a7 in ARPE-19 cells, which may play a specific role in the pathogenesis of proliferative vitreoretinopathy.

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